Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV system
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/1556 |
Resumo: | Developments in optical experimental techniques have helped in elucidating how blood flows through microvessels. Although initial developments were encouraging, studies on the flow properties of blood in microcirculation have been limited by several technical factors, such as poor spatial resolution and difficulty obtaining quantitative detailed measurements at such small scales. Recent advances in computing, microscopy, and digital image processing techniques have made it possible to combine a particle tracking velocimetry (PTV) system with a confocal microscope. We document the development of a confocal micro-PTV measurement system for capturing the dynamic flow behavior of red blood cells (RBCs) in concentrated suspensions. Measurements were performed at several depths through 100-mm glass capillaries. The confocal micro-PTV system was able to detect both translational and rotational motions of individual RBCs flowing in concentrated suspensions. Our results provide evidence that RBCs in dilute suspensions (3% hematocrit) tended to follow approximately linear trajectories, whereas RBCs in concentrated suspensions (20% hematocrit) exhibited transversal displacements of about 2% from the original path. Direct and quantitative measurements indicated that the plasma layer appeared to enhance the fluctuations in RBC trajectories owing to decreased obstruction in transversal movements caused by other RBCs. Using optical sectioning and subsequent image contrast and resolution enhancement, the system provides previously unobtainable information on the motion of RBCs, including the trajectories of two or more RBCs interacting in the same focal plane and RBC dispersion coefficients in different focal planes. |
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Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV systemRed blood cellsMicrocirculationMicrochannelBlood flowConfocal micro-PTVRBC dispersionDevelopments in optical experimental techniques have helped in elucidating how blood flows through microvessels. Although initial developments were encouraging, studies on the flow properties of blood in microcirculation have been limited by several technical factors, such as poor spatial resolution and difficulty obtaining quantitative detailed measurements at such small scales. Recent advances in computing, microscopy, and digital image processing techniques have made it possible to combine a particle tracking velocimetry (PTV) system with a confocal microscope. We document the development of a confocal micro-PTV measurement system for capturing the dynamic flow behavior of red blood cells (RBCs) in concentrated suspensions. Measurements were performed at several depths through 100-mm glass capillaries. The confocal micro-PTV system was able to detect both translational and rotational motions of individual RBCs flowing in concentrated suspensions. Our results provide evidence that RBCs in dilute suspensions (3% hematocrit) tended to follow approximately linear trajectories, whereas RBCs in concentrated suspensions (20% hematocrit) exhibited transversal displacements of about 2% from the original path. Direct and quantitative measurements indicated that the plasma layer appeared to enhance the fluctuations in RBC trajectories owing to decreased obstruction in transversal movements caused by other RBCs. Using optical sectioning and subsequent image contrast and resolution enhancement, the system provides previously unobtainable information on the motion of RBCs, including the trajectories of two or more RBCs interacting in the same focal plane and RBC dispersion coefficients in different focal planes.SpringerBiblioteca Digital do IPBLima, Rui A.Ishikawa, TakujiImai, YohsukeTakeda, MotohiroWada, ShigeoYamaguchi, Takami2010-01-27T20:29:13Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/1556engLima, R.; Ishikawa, T.; Imai, Y.; Takeda, M.; Wada, S.; Yamaguchi, T. (2009). Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV system. Annals of Biomedical Engineering. ISSN 1573-9686. 37:8, p.1546-15591573-9686info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:05:20Zoai:bibliotecadigital.ipb.pt:10198/1556Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:55:00.559981Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV system |
title |
Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV system |
spellingShingle |
Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV system Lima, Rui A. Red blood cells Microcirculation Microchannel Blood flow Confocal micro-PTV RBC dispersion |
title_short |
Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV system |
title_full |
Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV system |
title_fullStr |
Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV system |
title_full_unstemmed |
Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV system |
title_sort |
Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV system |
author |
Lima, Rui A. |
author_facet |
Lima, Rui A. Ishikawa, Takuji Imai, Yohsuke Takeda, Motohiro Wada, Shigeo Yamaguchi, Takami |
author_role |
author |
author2 |
Ishikawa, Takuji Imai, Yohsuke Takeda, Motohiro Wada, Shigeo Yamaguchi, Takami |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Lima, Rui A. Ishikawa, Takuji Imai, Yohsuke Takeda, Motohiro Wada, Shigeo Yamaguchi, Takami |
dc.subject.por.fl_str_mv |
Red blood cells Microcirculation Microchannel Blood flow Confocal micro-PTV RBC dispersion |
topic |
Red blood cells Microcirculation Microchannel Blood flow Confocal micro-PTV RBC dispersion |
description |
Developments in optical experimental techniques have helped in elucidating how blood flows through microvessels. Although initial developments were encouraging, studies on the flow properties of blood in microcirculation have been limited by several technical factors, such as poor spatial resolution and difficulty obtaining quantitative detailed measurements at such small scales. Recent advances in computing, microscopy, and digital image processing techniques have made it possible to combine a particle tracking velocimetry (PTV) system with a confocal microscope. We document the development of a confocal micro-PTV measurement system for capturing the dynamic flow behavior of red blood cells (RBCs) in concentrated suspensions. Measurements were performed at several depths through 100-mm glass capillaries. The confocal micro-PTV system was able to detect both translational and rotational motions of individual RBCs flowing in concentrated suspensions. Our results provide evidence that RBCs in dilute suspensions (3% hematocrit) tended to follow approximately linear trajectories, whereas RBCs in concentrated suspensions (20% hematocrit) exhibited transversal displacements of about 2% from the original path. Direct and quantitative measurements indicated that the plasma layer appeared to enhance the fluctuations in RBC trajectories owing to decreased obstruction in transversal movements caused by other RBCs. Using optical sectioning and subsequent image contrast and resolution enhancement, the system provides previously unobtainable information on the motion of RBCs, including the trajectories of two or more RBCs interacting in the same focal plane and RBC dispersion coefficients in different focal planes. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z 2010-01-27T20:29:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/1556 |
url |
http://hdl.handle.net/10198/1556 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lima, R.; Ishikawa, T.; Imai, Y.; Takeda, M.; Wada, S.; Yamaguchi, T. (2009). Measurement of individual red blood cell motions under high hematocrit conditions using a confocal micro-PTV system. Annals of Biomedical Engineering. ISSN 1573-9686. 37:8, p.1546-1559 1573-9686 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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