In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system

Detalhes bibliográficos
Autor(a) principal: Lima, Rui A.
Data de Publicação: 2008
Outros Autores: Wada, Shigeo, Tanaka, Shuji, Takeda, Motohiro, Ishikawa, Takuji, Tsubota, Ken-ichi, Imai, Yohsuke, Yamaguchi, Takami
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/609
Resumo: Progress in microfabricated technologies has attracted the attention of researchers in several areas, including microcirculation. Microfluidic devices are expected to provide powerful tools not only to better understand the biophysical behavior of blood flow in microvessels, but also for disease diagnosis. Such microfluidic devices for biomedical applications must be compatible with state-of-the-art flow measuring techniques, such as confocal microparticle image velocimetry (PIV). This confocal system has the ability to not only quantify flow patterns inside microchannels with high spatial and temporal resolution, but can also be used to obtain velocity measurements for several optically sectioned images along the depth of the microchannel. In this study, we investigated the ability to obtain velocity measurements using physiological saline (PS) and in vitro blood in a rectangular polydimethysiloxane (PDMS) microchannel (300 μm wide, 45 μm deep) using a confocal micro-PIV system. Applying this combination, measurements of trace particles seeded in the flow were performed for both fluids at a constant flow rate (Re = 0.02). Velocity profiles were acquired by successive measurements at different depth positions to obtain three-dimensional (3-D) information on the behavior of both fluid flows. Generally, the velocity profiles were found to be markedly blunt in the central region, mainly due to the low aspect ratio (h/w = 0.15) of the rectangular microchannel. Predictions using a theoretical model for the rectangular microchannel corresponded quite well with the experimental micro-PIV results for the PS fluid. However, for the in vitro blood with 20% hematocrit, small fluctuations were found in the velocity profiles. The present study clearly shows that confocal micro-PIV can be effectively integrated with a PDMS microchannel and used to obtain blood velocity profiles along the full depth of the microchannel because of its unique 3-D optical sectioning ability. Advantages and disadvantages of PDMS microchannels over glass capillaries are also discussed.
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spelling In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV systemMicrocirculationConfocal micro-PIVPDMS microchannelRed blood cellsMesoscopic blood flowProgress in microfabricated technologies has attracted the attention of researchers in several areas, including microcirculation. Microfluidic devices are expected to provide powerful tools not only to better understand the biophysical behavior of blood flow in microvessels, but also for disease diagnosis. Such microfluidic devices for biomedical applications must be compatible with state-of-the-art flow measuring techniques, such as confocal microparticle image velocimetry (PIV). This confocal system has the ability to not only quantify flow patterns inside microchannels with high spatial and temporal resolution, but can also be used to obtain velocity measurements for several optically sectioned images along the depth of the microchannel. In this study, we investigated the ability to obtain velocity measurements using physiological saline (PS) and in vitro blood in a rectangular polydimethysiloxane (PDMS) microchannel (300 μm wide, 45 μm deep) using a confocal micro-PIV system. Applying this combination, measurements of trace particles seeded in the flow were performed for both fluids at a constant flow rate (Re = 0.02). Velocity profiles were acquired by successive measurements at different depth positions to obtain three-dimensional (3-D) information on the behavior of both fluid flows. Generally, the velocity profiles were found to be markedly blunt in the central region, mainly due to the low aspect ratio (h/w = 0.15) of the rectangular microchannel. Predictions using a theoretical model for the rectangular microchannel corresponded quite well with the experimental micro-PIV results for the PS fluid. However, for the in vitro blood with 20% hematocrit, small fluctuations were found in the velocity profiles. The present study clearly shows that confocal micro-PIV can be effectively integrated with a PDMS microchannel and used to obtain blood velocity profiles along the full depth of the microchannel because of its unique 3-D optical sectioning ability. Advantages and disadvantages of PDMS microchannels over glass capillaries are also discussed.SpringerBiblioteca Digital do IPBLima, Rui A.Wada, ShigeoTanaka, ShujiTakeda, MotohiroIshikawa, TakujiTsubota, Ken-ichiImai, YohsukeYamaguchi, Takami2008-04-07T15:52:21Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/609engengLima, R.; Wada, S.; Tanaka, S.; Takeda, M.; Ishikawa, T.; Tsubota, K.; Imai, Y.; Yamaguchi, T. (2008). In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system. Biomedical Microdevices. ISSN 1387-2176. 10:2, p.153-1671387-2176Biomedical Microdevicesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:03:31Zoai:bibliotecadigital.ipb.pt:10198/609Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:54:18.504779Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system
title In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system
spellingShingle In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system
Lima, Rui A.
Microcirculation
Confocal micro-PIV
PDMS microchannel
Red blood cells
Mesoscopic blood flow
title_short In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system
title_full In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system
title_fullStr In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system
title_full_unstemmed In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system
title_sort In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system
author Lima, Rui A.
author_facet Lima, Rui A.
Wada, Shigeo
Tanaka, Shuji
Takeda, Motohiro
Ishikawa, Takuji
Tsubota, Ken-ichi
Imai, Yohsuke
Yamaguchi, Takami
author_role author
author2 Wada, Shigeo
Tanaka, Shuji
Takeda, Motohiro
Ishikawa, Takuji
Tsubota, Ken-ichi
Imai, Yohsuke
Yamaguchi, Takami
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Lima, Rui A.
Wada, Shigeo
Tanaka, Shuji
Takeda, Motohiro
Ishikawa, Takuji
Tsubota, Ken-ichi
Imai, Yohsuke
Yamaguchi, Takami
dc.subject.por.fl_str_mv Microcirculation
Confocal micro-PIV
PDMS microchannel
Red blood cells
Mesoscopic blood flow
topic Microcirculation
Confocal micro-PIV
PDMS microchannel
Red blood cells
Mesoscopic blood flow
description Progress in microfabricated technologies has attracted the attention of researchers in several areas, including microcirculation. Microfluidic devices are expected to provide powerful tools not only to better understand the biophysical behavior of blood flow in microvessels, but also for disease diagnosis. Such microfluidic devices for biomedical applications must be compatible with state-of-the-art flow measuring techniques, such as confocal microparticle image velocimetry (PIV). This confocal system has the ability to not only quantify flow patterns inside microchannels with high spatial and temporal resolution, but can also be used to obtain velocity measurements for several optically sectioned images along the depth of the microchannel. In this study, we investigated the ability to obtain velocity measurements using physiological saline (PS) and in vitro blood in a rectangular polydimethysiloxane (PDMS) microchannel (300 μm wide, 45 μm deep) using a confocal micro-PIV system. Applying this combination, measurements of trace particles seeded in the flow were performed for both fluids at a constant flow rate (Re = 0.02). Velocity profiles were acquired by successive measurements at different depth positions to obtain three-dimensional (3-D) information on the behavior of both fluid flows. Generally, the velocity profiles were found to be markedly blunt in the central region, mainly due to the low aspect ratio (h/w = 0.15) of the rectangular microchannel. Predictions using a theoretical model for the rectangular microchannel corresponded quite well with the experimental micro-PIV results for the PS fluid. However, for the in vitro blood with 20% hematocrit, small fluctuations were found in the velocity profiles. The present study clearly shows that confocal micro-PIV can be effectively integrated with a PDMS microchannel and used to obtain blood velocity profiles along the full depth of the microchannel because of its unique 3-D optical sectioning ability. Advantages and disadvantages of PDMS microchannels over glass capillaries are also discussed.
publishDate 2008
dc.date.none.fl_str_mv 2008-04-07T15:52:21Z
2008
2008-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/609
url http://hdl.handle.net/10198/609
dc.language.iso.fl_str_mv eng
eng
language eng
dc.relation.none.fl_str_mv Lima, R.; Wada, S.; Tanaka, S.; Takeda, M.; Ishikawa, T.; Tsubota, K.; Imai, Y.; Yamaguchi, T. (2008). In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system. Biomedical Microdevices. ISSN 1387-2176. 10:2, p.153-167
1387-2176
Biomedical Microdevices
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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