Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultry
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/48105 |
Resumo: | Campylobacter spp. are the most common bacterial pathogens associated with human gastroenteritis in industrialized countries. Contaminated chicken is the food vehicle associated with the majority of reported cases of campylobacteriosis, either by the consumption of undercooked meat or via cross- contamination of ready-to-eat (RTE) foods during the handling of contaminated raw chicken parts and carcasses. Our results indicate that cooking salt (used for seasoning) is a potential vehicle for Campylobacter spp. cross-contamination from raw chicken to lettuce, through unwashed hands after handling contaminated chicken. Cross-contamination events were observed even when the chicken skin was contaminated with low levels of Campylobacter spp. (ca. 1.48 Log CFU/g). The pathogen was recovered from seasoned lettuce samples when raw chicken was contaminated with levels ≥ 2.34 Log CFU/g. We also demonstrated that, once introduced into cooking salt, Campylobacter spp. are able to survive in a culturable state up to 4 hours. After six hours, although not detected following an enrichment period in culture medium, intact cells were observed by transmission electron microscopy. These findings reveal a "novel" indirect cross-contamination route of Campylobacter in domestic settings, and a putative contamination source to RTE foods that are seasoned with salt, that might occur if basic food hygiene practices are not adopted by consumers when preparing and cooking poultry dishes. |
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Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultryCampylobacter spp. are the most common bacterial pathogens associated with human gastroenteritis in industrialized countries. Contaminated chicken is the food vehicle associated with the majority of reported cases of campylobacteriosis, either by the consumption of undercooked meat or via cross- contamination of ready-to-eat (RTE) foods during the handling of contaminated raw chicken parts and carcasses. Our results indicate that cooking salt (used for seasoning) is a potential vehicle for Campylobacter spp. cross-contamination from raw chicken to lettuce, through unwashed hands after handling contaminated chicken. Cross-contamination events were observed even when the chicken skin was contaminated with low levels of Campylobacter spp. (ca. 1.48 Log CFU/g). The pathogen was recovered from seasoned lettuce samples when raw chicken was contaminated with levels ≥ 2.34 Log CFU/g. We also demonstrated that, once introduced into cooking salt, Campylobacter spp. are able to survive in a culturable state up to 4 hours. After six hours, although not detected following an enrichment period in culture medium, intact cells were observed by transmission electron microscopy. These findings reveal a "novel" indirect cross-contamination route of Campylobacter in domestic settings, and a putative contamination source to RTE foods that are seasoned with salt, that might occur if basic food hygiene practices are not adopted by consumers when preparing and cooking poultry dishes.Public Library of ScienceRepositório da Universidade de LisboaSantos, NanciAlves, ÂngelaCardoso, Maria JoãoLangsrud, SolveigMalheiro, Ana RitaFernandes, RuiMaia, RuiTruninger, MonicaJunqueira, LuisNicolau, Anca IoanaDumitrașcu, LoredanaSkuland, Silje ElisabethKasza, GyulaIzsó, TeklaFerreira, VâniaTeixeira, Paula2021-05-24T13:34:30Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/48105engSantos-Ferreira, N., Alves, Â., Cardoso, M. J., Langsrud, S., Malheiro, A. R., Fernandes, R., [...], Turninger, M., Junqueira, L., et al. (2021) Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultry. PLoS ONE 16(5): e0250980. https://doi.org/10.1371/journal.pone.02509801932-620310.1371/journal.pone.0250980info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:51:21Zoai:repositorio.ul.pt:10451/48105Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:02.004113Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultry |
title |
Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultry |
spellingShingle |
Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultry Santos, Nanci |
title_short |
Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultry |
title_full |
Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultry |
title_fullStr |
Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultry |
title_full_unstemmed |
Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultry |
title_sort |
Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultry |
author |
Santos, Nanci |
author_facet |
Santos, Nanci Alves, Ângela Cardoso, Maria João Langsrud, Solveig Malheiro, Ana Rita Fernandes, Rui Maia, Rui Truninger, Monica Junqueira, Luis Nicolau, Anca Ioana Dumitrașcu, Loredana Skuland, Silje Elisabeth Kasza, Gyula Izsó, Tekla Ferreira, Vânia Teixeira, Paula |
author_role |
author |
author2 |
Alves, Ângela Cardoso, Maria João Langsrud, Solveig Malheiro, Ana Rita Fernandes, Rui Maia, Rui Truninger, Monica Junqueira, Luis Nicolau, Anca Ioana Dumitrașcu, Loredana Skuland, Silje Elisabeth Kasza, Gyula Izsó, Tekla Ferreira, Vânia Teixeira, Paula |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Santos, Nanci Alves, Ângela Cardoso, Maria João Langsrud, Solveig Malheiro, Ana Rita Fernandes, Rui Maia, Rui Truninger, Monica Junqueira, Luis Nicolau, Anca Ioana Dumitrașcu, Loredana Skuland, Silje Elisabeth Kasza, Gyula Izsó, Tekla Ferreira, Vânia Teixeira, Paula |
description |
Campylobacter spp. are the most common bacterial pathogens associated with human gastroenteritis in industrialized countries. Contaminated chicken is the food vehicle associated with the majority of reported cases of campylobacteriosis, either by the consumption of undercooked meat or via cross- contamination of ready-to-eat (RTE) foods during the handling of contaminated raw chicken parts and carcasses. Our results indicate that cooking salt (used for seasoning) is a potential vehicle for Campylobacter spp. cross-contamination from raw chicken to lettuce, through unwashed hands after handling contaminated chicken. Cross-contamination events were observed even when the chicken skin was contaminated with low levels of Campylobacter spp. (ca. 1.48 Log CFU/g). The pathogen was recovered from seasoned lettuce samples when raw chicken was contaminated with levels ≥ 2.34 Log CFU/g. We also demonstrated that, once introduced into cooking salt, Campylobacter spp. are able to survive in a culturable state up to 4 hours. After six hours, although not detected following an enrichment period in culture medium, intact cells were observed by transmission electron microscopy. These findings reveal a "novel" indirect cross-contamination route of Campylobacter in domestic settings, and a putative contamination source to RTE foods that are seasoned with salt, that might occur if basic food hygiene practices are not adopted by consumers when preparing and cooking poultry dishes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-24T13:34:30Z 2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/48105 |
url |
http://hdl.handle.net/10451/48105 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos-Ferreira, N., Alves, Â., Cardoso, M. J., Langsrud, S., Malheiro, A. R., Fernandes, R., [...], Turninger, M., Junqueira, L., et al. (2021) Cross-contamination of lettuce with Campylobacter spp. via cooking salt during handling raw poultry. PLoS ONE 16(5): e0250980. https://doi.org/10.1371/journal.pone.0250980 1932-6203 10.1371/journal.pone.0250980 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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