Sources of endogenous glucose production in the Goto-Kakizaki diabetic rat
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/12713 https://doi.org/10.1016/j.diabet.2007.03.002 |
Resumo: | Plasma glucose, insulin and glucose tolerance were quantified in diabetic Goto–Kakizaki (GK) rats (342 ± 45 g, n = 5) and compared with weight-matched non-diabetic Wistars (307 ± 30 g, n = 8). Compared to Wistars, GK rats had higher fasting plasma insulin (219 ± 50 versus 44 ± 14 pmol/l, P < 0.002) and glucose (9.2 ± 2.3 versus 5.5 ± 0.5 mmol/l, P < 0.025). GK rats showed impaired glucose tolerance (IPGTT 2 h plasma glucose = 14 ± 1.5 versus 6.4 ± 0.1 mmol/l, P < 0.001). Endogenous glucose production (EGP) from glycogenolysis, phosphoenolpyruvate (PEP) and glycerol after 6 hours of fasting was quantified by a primed infusion of [U–13C]glucose and 2H2O tracers and 2H/13C NMR analysis of plasma glucose. EGP was higher in GK compared to Wistar rats (191 ± 16 versus 104 ± 27 μmol/kg per min, P < 0.005). This was sustained by increased gluconeogenesis from PEP (85 ± 12 versus 35 ± 4 μmol/kg per min, P < 0.02). Gluconeogenesis from glycerol was not different (20 ± 3 in Wistar versus 30 ± 6 μmol/kg per min for GK), and glycogenolysis fluxes were also not significantly different (76 ± 23 μmol/kg per min for GK versus 52 ± 19 μmol/kg per min for Wistar). The Cori cycle accounted for most of PEP gluconeogenesis in both Wistar and GK rats (85 ± 15% and 77 ± 10%, respectively). Therefore, increased gluconeogenesis in GK rats is largely sustained by increased Cori cycling while the maintenance of glycogenolysis indicates a failure in hepatic autoregulation of EGP |
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Sources of endogenous glucose production in the Goto-Kakizaki diabetic ratSources de production endogène du glucose chez le rat diabétique Goto–KakizakiEndogenous glucose productionGluconeogenesis[U–13C]glucoseType 2 diabetesGoto–Kakizaki ratsWistar ratsPlasma glucose, insulin and glucose tolerance were quantified in diabetic Goto–Kakizaki (GK) rats (342 ± 45 g, n = 5) and compared with weight-matched non-diabetic Wistars (307 ± 30 g, n = 8). Compared to Wistars, GK rats had higher fasting plasma insulin (219 ± 50 versus 44 ± 14 pmol/l, P < 0.002) and glucose (9.2 ± 2.3 versus 5.5 ± 0.5 mmol/l, P < 0.025). GK rats showed impaired glucose tolerance (IPGTT 2 h plasma glucose = 14 ± 1.5 versus 6.4 ± 0.1 mmol/l, P < 0.001). Endogenous glucose production (EGP) from glycogenolysis, phosphoenolpyruvate (PEP) and glycerol after 6 hours of fasting was quantified by a primed infusion of [U–13C]glucose and 2H2O tracers and 2H/13C NMR analysis of plasma glucose. EGP was higher in GK compared to Wistar rats (191 ± 16 versus 104 ± 27 μmol/kg per min, P < 0.005). This was sustained by increased gluconeogenesis from PEP (85 ± 12 versus 35 ± 4 μmol/kg per min, P < 0.02). Gluconeogenesis from glycerol was not different (20 ± 3 in Wistar versus 30 ± 6 μmol/kg per min for GK), and glycogenolysis fluxes were also not significantly different (76 ± 23 μmol/kg per min for GK versus 52 ± 19 μmol/kg per min for Wistar). The Cori cycle accounted for most of PEP gluconeogenesis in both Wistar and GK rats (85 ± 15% and 77 ± 10%, respectively). Therefore, increased gluconeogenesis in GK rats is largely sustained by increased Cori cycling while the maintenance of glycogenolysis indicates a failure in hepatic autoregulation of EGPLa glycémie, l'insulinémie et la tolérance au glucose ont été mesurées chez des rats diabétiques Goto–Kakizaki (GK; 342 ± 45 gr, n = 5), comparés à des rats non diabétiques Wistar (307 ± 30 gr, n = 8). Comparés aux rats Wistar, les rats GK avaient des valeurs plus élevées à jeun de la glycémie et de l'insulinémie (respectivement 219 ± 50 versus 44 ± 14 pmol/l, P < 0,002, et 9,2 ± 2,3 vs 5,5 ± 0,5 mmol/l, P < 0,025). Les rats GK présentaient une diminution de la tolérance au glucose (IPGTT 2 hr glucose plasmatique : 14 ± 1,5 versus 6,4 ± 0.1 mmol/l, P < 0,001). La production endogène de glucose (PEG) à partir de la glycogénolyse, de la phosphoénolpyruvate (PEP) et du glycérol, après 6 heures de jeûne, a été mesurée après une perfusion de [U–13C]glucose et du traceur 2H2O, et analyse par RMN de glucose 2H/13C plasmatique. La PEG était plus élevée chez les rats GK comparés aux rats Wistar (191 ± 16 versus 104 ± 27 μmol/kg per min, P < 0,005), du fait d'une augmentation de la néoglucogenèse à partir du PEG (85 ± 12 versus 35 ± 4 μmol/kg per min, P < 0,02). La néoglucogenèse à partir du glycérol n'était pas différente (20 ± 3 chez les rats Wistar vs 30 ± 6 μmol/kg per min chez les rats GK), alors que le flux de la glycogénolyse avait tendance à être plus élevé chez les rats GK (76 ± 23 μmol/kg per min), comparés aux rats Wistar (52 ± 19 μmol/kg per min, P = 0,06). Le cycle de Cori était responsable de la plus grande partie de la néoglucogenèse à partir de la PEP chez les deux types de rats, Wistar et GK (respectivement 85 ± 15 % et 77 ± 10 %). Ainsi, l'augmentation de la néoglucogenèse observée chez les rats GK est liée largement à l'augmentation du cycle de Cori, alors que le maintien de la glycogénolyse indique un déficit de l'autorégulation hépatique de la production endogène de glucoseElsevier Masson SAS2007-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/12713http://hdl.handle.net/10316/12713https://doi.org/10.1016/j.diabet.2007.03.002engDiabetes & Metabolism. 33:4 (2007) 296-3021262-3636Sena, C. M.Barosa, C.Nunes, E.Seiça, R.Jones, J. G.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-26T08:50:36Zoai:estudogeral.uc.pt:10316/12713Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:48.375452Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Sources of endogenous glucose production in the Goto-Kakizaki diabetic rat Sources de production endogène du glucose chez le rat diabétique Goto–Kakizaki |
title |
Sources of endogenous glucose production in the Goto-Kakizaki diabetic rat |
spellingShingle |
Sources of endogenous glucose production in the Goto-Kakizaki diabetic rat Sena, C. M. Endogenous glucose production Gluconeogenesis [U–13C]glucose Type 2 diabetes Goto–Kakizaki rats Wistar rats |
title_short |
Sources of endogenous glucose production in the Goto-Kakizaki diabetic rat |
title_full |
Sources of endogenous glucose production in the Goto-Kakizaki diabetic rat |
title_fullStr |
Sources of endogenous glucose production in the Goto-Kakizaki diabetic rat |
title_full_unstemmed |
Sources of endogenous glucose production in the Goto-Kakizaki diabetic rat |
title_sort |
Sources of endogenous glucose production in the Goto-Kakizaki diabetic rat |
author |
Sena, C. M. |
author_facet |
Sena, C. M. Barosa, C. Nunes, E. Seiça, R. Jones, J. G. |
author_role |
author |
author2 |
Barosa, C. Nunes, E. Seiça, R. Jones, J. G. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Sena, C. M. Barosa, C. Nunes, E. Seiça, R. Jones, J. G. |
dc.subject.por.fl_str_mv |
Endogenous glucose production Gluconeogenesis [U–13C]glucose Type 2 diabetes Goto–Kakizaki rats Wistar rats |
topic |
Endogenous glucose production Gluconeogenesis [U–13C]glucose Type 2 diabetes Goto–Kakizaki rats Wistar rats |
description |
Plasma glucose, insulin and glucose tolerance were quantified in diabetic Goto–Kakizaki (GK) rats (342 ± 45 g, n = 5) and compared with weight-matched non-diabetic Wistars (307 ± 30 g, n = 8). Compared to Wistars, GK rats had higher fasting plasma insulin (219 ± 50 versus 44 ± 14 pmol/l, P < 0.002) and glucose (9.2 ± 2.3 versus 5.5 ± 0.5 mmol/l, P < 0.025). GK rats showed impaired glucose tolerance (IPGTT 2 h plasma glucose = 14 ± 1.5 versus 6.4 ± 0.1 mmol/l, P < 0.001). Endogenous glucose production (EGP) from glycogenolysis, phosphoenolpyruvate (PEP) and glycerol after 6 hours of fasting was quantified by a primed infusion of [U–13C]glucose and 2H2O tracers and 2H/13C NMR analysis of plasma glucose. EGP was higher in GK compared to Wistar rats (191 ± 16 versus 104 ± 27 μmol/kg per min, P < 0.005). This was sustained by increased gluconeogenesis from PEP (85 ± 12 versus 35 ± 4 μmol/kg per min, P < 0.02). Gluconeogenesis from glycerol was not different (20 ± 3 in Wistar versus 30 ± 6 μmol/kg per min for GK), and glycogenolysis fluxes were also not significantly different (76 ± 23 μmol/kg per min for GK versus 52 ± 19 μmol/kg per min for Wistar). The Cori cycle accounted for most of PEP gluconeogenesis in both Wistar and GK rats (85 ± 15% and 77 ± 10%, respectively). Therefore, increased gluconeogenesis in GK rats is largely sustained by increased Cori cycling while the maintenance of glycogenolysis indicates a failure in hepatic autoregulation of EGP |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/12713 http://hdl.handle.net/10316/12713 https://doi.org/10.1016/j.diabet.2007.03.002 |
url |
http://hdl.handle.net/10316/12713 https://doi.org/10.1016/j.diabet.2007.03.002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diabetes & Metabolism. 33:4 (2007) 296-302 1262-3636 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier Masson SAS |
publisher.none.fl_str_mv |
Elsevier Masson SAS |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133844278870016 |