Expression of Desulfovibrio gigas desulforedoxin in Escherichia coli: Purification and characterization of mixed metal isoforms

Detalhes bibliográficos
Autor(a) principal: Czaja, Christopher
Data de Publicação: 1995
Outros Autores: Litwiller, Robert, Tomlinson, Andy J., Naylor, Stephen, Tavares, Pedro, LeGall, Jean, Moura, José J. G., Moura, Isabel, Rusnak, Frank
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/80768
Resumo: NIGMS NIH HHS (GM46865)
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spelling Expression of Desulfovibrio gigas desulforedoxin in Escherichia coli: Purification and characterization of mixed metal isoformsBiochemistryMolecular BiologyCell BiologyNIGMS NIH HHS (GM46865)The dsr gene from Desulfovibrio gigas encoding the nonheme iron protein desulforedoxin was cloned using the polymerase chain reaction, expressed in Escherichia coli, and purified to homogeneity. The physical and spectroscopic properties of the recombinant protein resemble those observed for the native protein isolated from D. gigas. These include an α2 tertiary structure, the presence of bound iron, and absorbance maxima at 370 and 506 nm in the UV/visible spectrum due to ligand-to-iron charge transfer bands. Low temperature electron paramagnetic resonance studies confirm the presence of a high-spin ferric ion with g values of 7.7, 5.7, 4.1, and 1.8. Interestingly, E. coli produced two forms of desulforedoxin containing iron. One form was identified as a dimer with the metal-binding sites of both subunits occupied by iron while the second form contained equivalent amounts of iron and zinc and represents a dimer with one subunit occupied by iron and the second with zinc.DQ - Departamento de QuímicaCQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)RUNCzaja, ChristopherLitwiller, RobertTomlinson, Andy J.Naylor, StephenTavares, PedroLeGall, JeanMoura, José J. G.Moura, IsabelRusnak, Frank2019-09-10T22:49:32Z1995-09-011995-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5application/pdfhttp://hdl.handle.net/10362/80768eng0021-9258PURE: 14634080http://www.scopus.com/inward/record.url?scp=0029021221&partnerID=8YFLogxKhttps://doi.org/10.1074/jbc.270.35.20273info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:35:45Zoai:run.unl.pt:10362/80768Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:55.714203Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Expression of Desulfovibrio gigas desulforedoxin in Escherichia coli: Purification and characterization of mixed metal isoforms
title Expression of Desulfovibrio gigas desulforedoxin in Escherichia coli: Purification and characterization of mixed metal isoforms
spellingShingle Expression of Desulfovibrio gigas desulforedoxin in Escherichia coli: Purification and characterization of mixed metal isoforms
Czaja, Christopher
Biochemistry
Molecular Biology
Cell Biology
title_short Expression of Desulfovibrio gigas desulforedoxin in Escherichia coli: Purification and characterization of mixed metal isoforms
title_full Expression of Desulfovibrio gigas desulforedoxin in Escherichia coli: Purification and characterization of mixed metal isoforms
title_fullStr Expression of Desulfovibrio gigas desulforedoxin in Escherichia coli: Purification and characterization of mixed metal isoforms
title_full_unstemmed Expression of Desulfovibrio gigas desulforedoxin in Escherichia coli: Purification and characterization of mixed metal isoforms
title_sort Expression of Desulfovibrio gigas desulforedoxin in Escherichia coli: Purification and characterization of mixed metal isoforms
author Czaja, Christopher
author_facet Czaja, Christopher
Litwiller, Robert
Tomlinson, Andy J.
Naylor, Stephen
Tavares, Pedro
LeGall, Jean
Moura, José J. G.
Moura, Isabel
Rusnak, Frank
author_role author
author2 Litwiller, Robert
Tomlinson, Andy J.
Naylor, Stephen
Tavares, Pedro
LeGall, Jean
Moura, José J. G.
Moura, Isabel
Rusnak, Frank
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)
RUN
dc.contributor.author.fl_str_mv Czaja, Christopher
Litwiller, Robert
Tomlinson, Andy J.
Naylor, Stephen
Tavares, Pedro
LeGall, Jean
Moura, José J. G.
Moura, Isabel
Rusnak, Frank
dc.subject.por.fl_str_mv Biochemistry
Molecular Biology
Cell Biology
topic Biochemistry
Molecular Biology
Cell Biology
description NIGMS NIH HHS (GM46865)
publishDate 1995
dc.date.none.fl_str_mv 1995-09-01
1995-09-01T00:00:00Z
2019-09-10T22:49:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/80768
url http://hdl.handle.net/10362/80768
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9258
PURE: 14634080
http://www.scopus.com/inward/record.url?scp=0029021221&partnerID=8YFLogxK
https://doi.org/10.1074/jbc.270.35.20273
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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