Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean

Detalhes bibliográficos
Autor(a) principal: Díaz-Formoso, Lara
Data de Publicação: 2023
Outros Autores: Silva, Vanessa, Contente, Diogo, Feito, Javier, Hernández, Pablo E., Borrero, Juan, Igrejas, Gilberto, Campo, Rosa del, Muñoz-Atienza, Estefanía, Poeta, Patrícia, Cintas, Luis M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/164617
Resumo: Funding Information: This research was funded by the Ministerio de Ciencia, Innovación y Universidades (MCIU, Spain, Projects RTI2018-094907-B-I00 and PID2019-104808RA-I00), and Fundação para a Ciência e a Tecnologia (FCT, Portugal, UIDB/50006/2020, UIDP/50006/2020, UIDB/CVT/00772/2020, and LA/P/0059/2020). L.D.F. was supported by the Programa Investigo (Ministerio de Trabajo y Economía Social, MITES, Spain), funded by the EU (NextGenerationEU). V.S. was supported by a PhD grant (SFRH/BD/137947/2018FCT) from FCT (Portugal). D.C. was supported by a contract from the project RTI2018-094907-B-I00 (MCIU, Spain). J.F. was supported by a FEI16/54 contract from the Universidad Complutense de Madrid (UCM, Spain) and held a predoctoral contract from the UCM. JB was supported by the Programa Atracción de Talento (2018-T1/BIO-10158) from the Comunidad de Madrid (Spain). Publisher Copyright: © 2023 by the authors.
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spelling Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Oceanantibiotic resistanceantimicrobial activitybiofilm formationEuropean hakes (Merluccius merluccius, L.)Staphylococcussppvirulence factorsImmunology and AllergyMolecular BiologyImmunology and Microbiology(all)Microbiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: This research was funded by the Ministerio de Ciencia, Innovación y Universidades (MCIU, Spain, Projects RTI2018-094907-B-I00 and PID2019-104808RA-I00), and Fundação para a Ciência e a Tecnologia (FCT, Portugal, UIDB/50006/2020, UIDP/50006/2020, UIDB/CVT/00772/2020, and LA/P/0059/2020). L.D.F. was supported by the Programa Investigo (Ministerio de Trabajo y Economía Social, MITES, Spain), funded by the EU (NextGenerationEU). V.S. was supported by a PhD grant (SFRH/BD/137947/2018FCT) from FCT (Portugal). D.C. was supported by a contract from the project RTI2018-094907-B-I00 (MCIU, Spain). J.F. was supported by a FEI16/54 contract from the Universidad Complutense de Madrid (UCM, Spain) and held a predoctoral contract from the UCM. JB was supported by the Programa Atracción de Talento (2018-T1/BIO-10158) from the Comunidad de Madrid (Spain). Publisher Copyright: © 2023 by the authors.The indiscriminate use of antibiotics has contributed to the dissemination of multiresistant bacteria, which represents a public health concern. The aim of this work was to characterize 27 coagulase-negative staphylococci (CoNS) isolated from eight wild Northeast Atlantic hakes (Merluccius merluccius, L.) and taxonomically identified as Staphylococcus epidermidis (n = 16), Staphylococcus saprophyticus (n = 4), Staphylococcus hominis (n = 3), Staphylococcus pasteuri (n = 2), Staphylococcus edaphicus (n = 1), and Staphylococcus capitis (n = 1). Biofilm formation was evaluated with a microtiter assay, antibiotic susceptibility testing was performed using the disk diffusion method, and antibiotic resistance and virulence determinants were detected by PCR. Our results showed that all staphylococci produced biofilms and that 92.6% of the isolates were resistant to at least one antibiotic, mainly penicillin (88.8%), fusidic acid (40.7%), and erythromycin (37%). The penicillin resistance gene (blaZ) was detected in 66.6% (18) of the isolates, of which 10 also carried resistance genes to macrolides and lincosamides (mphC, msr(A/B), lnuA, or vgaA), 4 to fusidic acid (fusB), and 3 to trimethoprim-sulfamethoxazole (dfrA). At least one virulence gene (scn, hla, SCCmecIII, and/or SCCmecV) was detected in 48% of the isolates. This study suggests that wild European hake destined for human consumption could act as a vector of CoNS carrying antibiotic resistance genes and/or virulence factors.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNDíaz-Formoso, LaraSilva, VanessaContente, DiogoFeito, JavierHernández, Pablo E.Borrero, JuanIgrejas, GilbertoCampo, Rosa delMuñoz-Atienza, EstefaníaPoeta, PatríciaCintas, Luis M.2024-03-07T23:56:24Z2023-12-132023-12-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/164617eng2076-0817PURE: 83897123https://doi.org/10.3390/pathogens12121447info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:53:27Zoai:run.unl.pt:10362/164617Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:00:16.962158Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean
title Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean
spellingShingle Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean
Díaz-Formoso, Lara
antibiotic resistance
antimicrobial activity
biofilm formation
European hakes (Merluccius merluccius, L.)
Staphylococcusspp
virulence factors
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean
title_full Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean
title_fullStr Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean
title_full_unstemmed Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean
title_sort Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean
author Díaz-Formoso, Lara
author_facet Díaz-Formoso, Lara
Silva, Vanessa
Contente, Diogo
Feito, Javier
Hernández, Pablo E.
Borrero, Juan
Igrejas, Gilberto
Campo, Rosa del
Muñoz-Atienza, Estefanía
Poeta, Patrícia
Cintas, Luis M.
author_role author
author2 Silva, Vanessa
Contente, Diogo
Feito, Javier
Hernández, Pablo E.
Borrero, Juan
Igrejas, Gilberto
Campo, Rosa del
Muñoz-Atienza, Estefanía
Poeta, Patrícia
Cintas, Luis M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Díaz-Formoso, Lara
Silva, Vanessa
Contente, Diogo
Feito, Javier
Hernández, Pablo E.
Borrero, Juan
Igrejas, Gilberto
Campo, Rosa del
Muñoz-Atienza, Estefanía
Poeta, Patrícia
Cintas, Luis M.
dc.subject.por.fl_str_mv antibiotic resistance
antimicrobial activity
biofilm formation
European hakes (Merluccius merluccius, L.)
Staphylococcusspp
virulence factors
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
topic antibiotic resistance
antimicrobial activity
biofilm formation
European hakes (Merluccius merluccius, L.)
Staphylococcusspp
virulence factors
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
description Funding Information: This research was funded by the Ministerio de Ciencia, Innovación y Universidades (MCIU, Spain, Projects RTI2018-094907-B-I00 and PID2019-104808RA-I00), and Fundação para a Ciência e a Tecnologia (FCT, Portugal, UIDB/50006/2020, UIDP/50006/2020, UIDB/CVT/00772/2020, and LA/P/0059/2020). L.D.F. was supported by the Programa Investigo (Ministerio de Trabajo y Economía Social, MITES, Spain), funded by the EU (NextGenerationEU). V.S. was supported by a PhD grant (SFRH/BD/137947/2018FCT) from FCT (Portugal). D.C. was supported by a contract from the project RTI2018-094907-B-I00 (MCIU, Spain). J.F. was supported by a FEI16/54 contract from the Universidad Complutense de Madrid (UCM, Spain) and held a predoctoral contract from the UCM. JB was supported by the Programa Atracción de Talento (2018-T1/BIO-10158) from the Comunidad de Madrid (Spain). Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-13
2023-12-13T00:00:00Z
2024-03-07T23:56:24Z
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 2076-0817
PURE: 83897123
https://doi.org/10.3390/pathogens12121447
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