Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/164617 |
Resumo: | Funding Information: This research was funded by the Ministerio de Ciencia, Innovación y Universidades (MCIU, Spain, Projects RTI2018-094907-B-I00 and PID2019-104808RA-I00), and Fundação para a Ciência e a Tecnologia (FCT, Portugal, UIDB/50006/2020, UIDP/50006/2020, UIDB/CVT/00772/2020, and LA/P/0059/2020). L.D.F. was supported by the Programa Investigo (Ministerio de Trabajo y Economía Social, MITES, Spain), funded by the EU (NextGenerationEU). V.S. was supported by a PhD grant (SFRH/BD/137947/2018FCT) from FCT (Portugal). D.C. was supported by a contract from the project RTI2018-094907-B-I00 (MCIU, Spain). J.F. was supported by a FEI16/54 contract from the Universidad Complutense de Madrid (UCM, Spain) and held a predoctoral contract from the UCM. JB was supported by the Programa Atracción de Talento (2018-T1/BIO-10158) from the Comunidad de Madrid (Spain). Publisher Copyright: © 2023 by the authors. |
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Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Oceanantibiotic resistanceantimicrobial activitybiofilm formationEuropean hakes (Merluccius merluccius, L.)Staphylococcussppvirulence factorsImmunology and AllergyMolecular BiologyImmunology and Microbiology(all)Microbiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: This research was funded by the Ministerio de Ciencia, Innovación y Universidades (MCIU, Spain, Projects RTI2018-094907-B-I00 and PID2019-104808RA-I00), and Fundação para a Ciência e a Tecnologia (FCT, Portugal, UIDB/50006/2020, UIDP/50006/2020, UIDB/CVT/00772/2020, and LA/P/0059/2020). L.D.F. was supported by the Programa Investigo (Ministerio de Trabajo y Economía Social, MITES, Spain), funded by the EU (NextGenerationEU). V.S. was supported by a PhD grant (SFRH/BD/137947/2018FCT) from FCT (Portugal). D.C. was supported by a contract from the project RTI2018-094907-B-I00 (MCIU, Spain). J.F. was supported by a FEI16/54 contract from the Universidad Complutense de Madrid (UCM, Spain) and held a predoctoral contract from the UCM. JB was supported by the Programa Atracción de Talento (2018-T1/BIO-10158) from the Comunidad de Madrid (Spain). Publisher Copyright: © 2023 by the authors.The indiscriminate use of antibiotics has contributed to the dissemination of multiresistant bacteria, which represents a public health concern. The aim of this work was to characterize 27 coagulase-negative staphylococci (CoNS) isolated from eight wild Northeast Atlantic hakes (Merluccius merluccius, L.) and taxonomically identified as Staphylococcus epidermidis (n = 16), Staphylococcus saprophyticus (n = 4), Staphylococcus hominis (n = 3), Staphylococcus pasteuri (n = 2), Staphylococcus edaphicus (n = 1), and Staphylococcus capitis (n = 1). Biofilm formation was evaluated with a microtiter assay, antibiotic susceptibility testing was performed using the disk diffusion method, and antibiotic resistance and virulence determinants were detected by PCR. Our results showed that all staphylococci produced biofilms and that 92.6% of the isolates were resistant to at least one antibiotic, mainly penicillin (88.8%), fusidic acid (40.7%), and erythromycin (37%). The penicillin resistance gene (blaZ) was detected in 66.6% (18) of the isolates, of which 10 also carried resistance genes to macrolides and lincosamides (mphC, msr(A/B), lnuA, or vgaA), 4 to fusidic acid (fusB), and 3 to trimethoprim-sulfamethoxazole (dfrA). At least one virulence gene (scn, hla, SCCmecIII, and/or SCCmecV) was detected in 48% of the isolates. This study suggests that wild European hake destined for human consumption could act as a vector of CoNS carrying antibiotic resistance genes and/or virulence factors.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNDíaz-Formoso, LaraSilva, VanessaContente, DiogoFeito, JavierHernández, Pablo E.Borrero, JuanIgrejas, GilbertoCampo, Rosa delMuñoz-Atienza, EstefaníaPoeta, PatríciaCintas, Luis M.2024-03-07T23:56:24Z2023-12-132023-12-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/164617eng2076-0817PURE: 83897123https://doi.org/10.3390/pathogens12121447info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:53:27Zoai:run.unl.pt:10362/164617Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:00:16.962158Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean |
title |
Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean |
spellingShingle |
Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean Díaz-Formoso, Lara antibiotic resistance antimicrobial activity biofilm formation European hakes (Merluccius merluccius, L.) Staphylococcusspp virulence factors Immunology and Allergy Molecular Biology Immunology and Microbiology(all) Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
title_short |
Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean |
title_full |
Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean |
title_fullStr |
Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean |
title_full_unstemmed |
Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean |
title_sort |
Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean |
author |
Díaz-Formoso, Lara |
author_facet |
Díaz-Formoso, Lara Silva, Vanessa Contente, Diogo Feito, Javier Hernández, Pablo E. Borrero, Juan Igrejas, Gilberto Campo, Rosa del Muñoz-Atienza, Estefanía Poeta, Patrícia Cintas, Luis M. |
author_role |
author |
author2 |
Silva, Vanessa Contente, Diogo Feito, Javier Hernández, Pablo E. Borrero, Juan Igrejas, Gilberto Campo, Rosa del Muñoz-Atienza, Estefanía Poeta, Patrícia Cintas, Luis M. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
LAQV@REQUIMTE DQ - Departamento de Química RUN |
dc.contributor.author.fl_str_mv |
Díaz-Formoso, Lara Silva, Vanessa Contente, Diogo Feito, Javier Hernández, Pablo E. Borrero, Juan Igrejas, Gilberto Campo, Rosa del Muñoz-Atienza, Estefanía Poeta, Patrícia Cintas, Luis M. |
dc.subject.por.fl_str_mv |
antibiotic resistance antimicrobial activity biofilm formation European hakes (Merluccius merluccius, L.) Staphylococcusspp virulence factors Immunology and Allergy Molecular Biology Immunology and Microbiology(all) Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
topic |
antibiotic resistance antimicrobial activity biofilm formation European hakes (Merluccius merluccius, L.) Staphylococcusspp virulence factors Immunology and Allergy Molecular Biology Immunology and Microbiology(all) Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
description |
Funding Information: This research was funded by the Ministerio de Ciencia, Innovación y Universidades (MCIU, Spain, Projects RTI2018-094907-B-I00 and PID2019-104808RA-I00), and Fundação para a Ciência e a Tecnologia (FCT, Portugal, UIDB/50006/2020, UIDP/50006/2020, UIDB/CVT/00772/2020, and LA/P/0059/2020). L.D.F. was supported by the Programa Investigo (Ministerio de Trabajo y Economía Social, MITES, Spain), funded by the EU (NextGenerationEU). V.S. was supported by a PhD grant (SFRH/BD/137947/2018FCT) from FCT (Portugal). D.C. was supported by a contract from the project RTI2018-094907-B-I00 (MCIU, Spain). J.F. was supported by a FEI16/54 contract from the Universidad Complutense de Madrid (UCM, Spain) and held a predoctoral contract from the UCM. JB was supported by the Programa Atracción de Talento (2018-T1/BIO-10158) from the Comunidad de Madrid (Spain). Publisher Copyright: © 2023 by the authors. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-13 2023-12-13T00:00:00Z 2024-03-07T23:56:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/164617 |
url |
http://hdl.handle.net/10362/164617 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2076-0817 PURE: 83897123 https://doi.org/10.3390/pathogens12121447 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799138178511142912 |