Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

Detalhes bibliográficos
Autor(a) principal: Gouveia-Caridade, Carla
Data de Publicação: 2008
Outros Autores: Pauliukaite, Rasa, Brett, Christopher M. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/5285
https://doi.org/10.1016/j.electacta.2008.01.040
Resumo: Functionalised multi-walled carbon nanotubes (MWCNTs) were cast on glassy carbon (GC) and carbon film electrodes (CFE), and were characterised electrochemically and applied in a glucose-oxidase-based biosensor. MWCNT-modified carbon film electrodes were then used to develop an alcohol oxidase (AlcOx) biosensor, in which AlcOx-BSA was cross-linked with glutaraldehyde and attached by drop-coating. The experimental conditions, applied potential and pH, for ethanol monitoring were optimised, and ethanol was determined amperometrically at -0.3 V vs. SCE at pH 7.5. Electrocatalytic effects of MWCNT were observed with respect to unmodified carbon film electrodes. The sensitivity obtained was 20 times higher at carbon film/MWCNT-based biosensors than without MWCNT.
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spelling Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanolMulti-walled carbon nanotubesCarbon film electrodesGlucose oxidaseAlcohol oxidaseElectrochemical biosensorFunctionalised multi-walled carbon nanotubes (MWCNTs) were cast on glassy carbon (GC) and carbon film electrodes (CFE), and were characterised electrochemically and applied in a glucose-oxidase-based biosensor. MWCNT-modified carbon film electrodes were then used to develop an alcohol oxidase (AlcOx) biosensor, in which AlcOx-BSA was cross-linked with glutaraldehyde and attached by drop-coating. The experimental conditions, applied potential and pH, for ethanol monitoring were optimised, and ethanol was determined amperometrically at -0.3 V vs. SCE at pH 7.5. Electrocatalytic effects of MWCNT were observed with respect to unmodified carbon film electrodes. The sensitivity obtained was 20 times higher at carbon film/MWCNT-based biosensors than without MWCNT.http://www.sciencedirect.com/science/article/B6TG0-4RPM7TX-1/1/c7c201831d528c29b469d52592bc65612008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5285http://hdl.handle.net/10316/5285https://doi.org/10.1016/j.electacta.2008.01.040engElectrochimica Acta. 53:23 (2008) 6732-6739Gouveia-Caridade, CarlaPauliukaite, RasaBrett, Christopher M. A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-07-27T09:47:03Zoai:estudogeral.uc.pt:10316/5285Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:24.286023Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol
title Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol
spellingShingle Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol
Gouveia-Caridade, Carla
Multi-walled carbon nanotubes
Carbon film electrodes
Glucose oxidase
Alcohol oxidase
Electrochemical biosensor
title_short Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol
title_full Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol
title_fullStr Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol
title_full_unstemmed Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol
title_sort Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol
author Gouveia-Caridade, Carla
author_facet Gouveia-Caridade, Carla
Pauliukaite, Rasa
Brett, Christopher M. A.
author_role author
author2 Pauliukaite, Rasa
Brett, Christopher M. A.
author2_role author
author
dc.contributor.author.fl_str_mv Gouveia-Caridade, Carla
Pauliukaite, Rasa
Brett, Christopher M. A.
dc.subject.por.fl_str_mv Multi-walled carbon nanotubes
Carbon film electrodes
Glucose oxidase
Alcohol oxidase
Electrochemical biosensor
topic Multi-walled carbon nanotubes
Carbon film electrodes
Glucose oxidase
Alcohol oxidase
Electrochemical biosensor
description Functionalised multi-walled carbon nanotubes (MWCNTs) were cast on glassy carbon (GC) and carbon film electrodes (CFE), and were characterised electrochemically and applied in a glucose-oxidase-based biosensor. MWCNT-modified carbon film electrodes were then used to develop an alcohol oxidase (AlcOx) biosensor, in which AlcOx-BSA was cross-linked with glutaraldehyde and attached by drop-coating. The experimental conditions, applied potential and pH, for ethanol monitoring were optimised, and ethanol was determined amperometrically at -0.3 V vs. SCE at pH 7.5. Electrocatalytic effects of MWCNT were observed with respect to unmodified carbon film electrodes. The sensitivity obtained was 20 times higher at carbon film/MWCNT-based biosensors than without MWCNT.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/5285
http://hdl.handle.net/10316/5285
https://doi.org/10.1016/j.electacta.2008.01.040
url http://hdl.handle.net/10316/5285
https://doi.org/10.1016/j.electacta.2008.01.040
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Electrochimica Acta. 53:23 (2008) 6732-6739
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv aplication/PDF
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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