Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer

Detalhes bibliográficos
Autor(a) principal: Araújo, Diana
Data de Publicação: 2020
Outros Autores: Alves, Vítor D., Marques, Ana C., Fortunato, Elvira, Reis, Maria A. M., Freitas, Filomena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/114326
Resumo: UIDB/04378/2020 UID/AGR/04129/2020 UID/CTM/50025/2019 BD/140829/2018 SFRH/BD/115173/2016
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spelling Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymerChitin-glucan complex (CGC)Chitosan-glucan complex (ChGC)DissolutionNaOH/urea solvent systemsStructural analysisThermal propertiesBioengineeringUIDB/04378/2020 UID/AGR/04129/2020 UID/CTM/50025/2019 BD/140829/2018 SFRH/BD/115173/2016Chitin-glucan complex (CGC) is a copolymer composed of chitin and glucan moieties extracted from the cell-walls of several yeasts and fungi. Despite its proven valuable properties, that include antibacterial, antioxidant and anticancer activity, the utilization of CGC in many applications is hindered by its insolubility in water and most solvents. In this study, NaOH/urea solvent systems were used for the first time for solubilization of CGC extracted from the yeast Komagataella pastoris. Different NaOH/urea ratios (6:8, 8:4 and 11:4 (w/w), respectively) were used to obtain aqueous solutions using a freeze/thaw procedure. There was an overall solubilization of 63–68%, with the highest solubilization rate obtained for the highest tested urea concentration (8 wt%). The regenerated polymer, obtained by dialysis of the alkali solutions followed by lyophilization, formed porous macrostructures characterized by a chemical composition similar to that of the starting co-polymer, although the acetylation degree decreased from 61.3% to 33.9–50.6%, indicating that chitin was converted into chitosan, yielding chitosan-glucan complex (ChGC). Consistent with this, there was a reduction of the crystallinity index and thermal degradation temperature. Given these results, this study reports a simple and green procedure to solubilize CGC and obtain aqueous ChGC solutions that can be processed as novel biomaterials.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaUNINOVA-Instituto de Desenvolvimento de Novas TecnologiasCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisRUNAraújo, DianaAlves, Vítor D.Marques, Ana C.Fortunato, ElviraReis, Maria A. M.Freitas, Filomena2021-03-23T23:24:25Z2020-03-142020-03-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/114326eng2306-5354PURE: 18017744https://doi.org/10.3390/bioengineering7010028info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:56:56Zoai:run.unl.pt:10362/114326Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:29.457993Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer
title Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer
spellingShingle Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer
Araújo, Diana
Chitin-glucan complex (CGC)
Chitosan-glucan complex (ChGC)
Dissolution
NaOH/urea solvent systems
Structural analysis
Thermal properties
Bioengineering
title_short Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer
title_full Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer
title_fullStr Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer
title_full_unstemmed Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer
title_sort Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer
author Araújo, Diana
author_facet Araújo, Diana
Alves, Vítor D.
Marques, Ana C.
Fortunato, Elvira
Reis, Maria A. M.
Freitas, Filomena
author_role author
author2 Alves, Vítor D.
Marques, Ana C.
Fortunato, Elvira
Reis, Maria A. M.
Freitas, Filomena
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
DCM - Departamento de Ciência dos Materiais
RUN
dc.contributor.author.fl_str_mv Araújo, Diana
Alves, Vítor D.
Marques, Ana C.
Fortunato, Elvira
Reis, Maria A. M.
Freitas, Filomena
dc.subject.por.fl_str_mv Chitin-glucan complex (CGC)
Chitosan-glucan complex (ChGC)
Dissolution
NaOH/urea solvent systems
Structural analysis
Thermal properties
Bioengineering
topic Chitin-glucan complex (CGC)
Chitosan-glucan complex (ChGC)
Dissolution
NaOH/urea solvent systems
Structural analysis
Thermal properties
Bioengineering
description UIDB/04378/2020 UID/AGR/04129/2020 UID/CTM/50025/2019 BD/140829/2018 SFRH/BD/115173/2016
publishDate 2020
dc.date.none.fl_str_mv 2020-03-14
2020-03-14T00:00:00Z
2021-03-23T23:24:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/114326
url http://hdl.handle.net/10362/114326
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2306-5354
PURE: 18017744
https://doi.org/10.3390/bioengineering7010028
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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