The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.7/777 |
Resumo: | This paper version is the accepted manuscript posted online 21 July 2017. It has peer-review. This publication hasn't any creative commons license associated. The deposited article version contains attached the supplementary materials within the pdf. |
| id |
RCAP_72d32f33f677602284d9a489adec8b29 |
|---|---|
| oai_identifier_str |
oai:arca.igc.gulbenkian.pt:10400.7/777 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formationLysR-family transcriptional regulatorD-lactate dehydrogenaseBurkholderia multivoransPlanktonic cellular aggregatesBiofilmsExopolysaccharideCystic fibrosisThis paper version is the accepted manuscript posted online 21 July 2017. It has peer-review. This publication hasn't any creative commons license associated. The deposited article version contains attached the supplementary materials within the pdf.LysR-type transcriptional regulators (LTTR) are the most commonly found regulators in Burkholderia cepacia complex, comprising opportunistic pathogens causing chronic respiratory infections in cystic fibrosis (CF) patients. Despite LTTRs being global regulators of pathogenicity in several bacteria, few have been characterized in Burkholderia Here, we showed that gene ldhR of B. multivorans encoding a LTTR is co-transcribed with ldhA encoding a d-lactate dehydrogenase, and evaluate their implication in virulence traits like exopolysaccharide (EPS) synthesis and biofilm formation. Comparison of wild-type (WT) and its isogenic ΔldhR mutant grown in medium with 2% d-glucose revealed a negative impact on EPS biosynthesis and on cells' viability in the presence of LdhR. Loss of viability in WT cells was caused by intracellular acidification as consequence of cumulative organic acids secretion including d-lactate, this last one absent from the ΔldhR mutant supernatant. Furthermore, LdhR is implicated in the formation of planktonic cellular aggregates. WT cell aggregates reached 1000 μm after 24 hours in liquid cultures; in contrast to ΔldhR mutant aggregates that never grew more than 60 μm. Overexpression of d-lactate dehydrogenase LdhA in the ΔldhR mutant partially restored formed aggregates size, suggesting a role for fermentation inside aggregates. Similar results were obtained for surface-attached biofilms, with WT cells producing more biofilm. A systematic evaluation of planktonic aggregates in Burkholderia CF clinical isolates showed aggregates in 40 out of 74. As CF patients' lung environment is microaerophilic and bacteria are found as free aggregates/biofilms, LdhR and LdhA might have central roles in adaptation to this environment.IMPORTANCE Cystic fibrosis patients often suffer from chronic respiratory infections caused by several microorganisms. Among them are the Burkholderia cepacia complex bacteria which cause progressive deterioration of lung function and, in some patients, might develop into fatal necrotizing pneumoniae with bacteremia, known as "cepacia syndrome". Burkholderia pathogenesis is multifactorial since they express several virulence factors, form biofilms, and are highly resistant to antimicrobial compounds, making their eradication from the CF patients' airways very difficult. As Burkholderia is commonly found in the CF lungs in the form of cell aggregates and biofilms, the need to investigate the mechanisms of cellular aggregation is obvious. In this study we demonstrate the importance of a d-lactate dehydrogenase and a regulator, in regulating carbon overflow, cellular aggregates and surface-attached biofilm formation. This not only enhances our understanding of Burkholderia pathogenesis, but can also lead to the development of drugs against these proteins to circumvent biofilm formation.Instituto Gulbenkian de Ciência (Gene Expression Unit); Programa Operacional 833 Regional de Lisboa 2020 grant: (LISBOA-01-0145-FEDER-007317); Fundação para a Ciência e a Tecnologia grants: (PTDC/QUI-BIQ/118260/2010, UID/BIO/04565/2013, SFRH/BPD/86475/2012).American Society for MicrobiologyARCASilva, Inês N.Ramires, Marcelo J.Azevedo, Lisa A.Guerreiro, Ana R.Tavares, Andreia C.Becker, Jörg D.Moreira, Leonilde M.2018-08-01T00:30:08Z2017-072017-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/777engThe regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formation Inês N. Silva, Marcelo J. Ramires, Lisa A. Azevedo, Ana R. Guerreiro, Andreia C. Tavares, Jörg D. Becker, and Leonilde M. Moreira Appl. Environ. Microbiol. AEM.01343-17; Accepted manuscript posted online 21 July 2017, doi:10.1128/AEM.01343-1710.1128/AEM.01343-17info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:10Zoai:arca.igc.gulbenkian.pt:10400.7/777Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:12:00.242480Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formation |
| title |
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formation |
| spellingShingle |
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formation Silva, Inês N. LysR-family transcriptional regulator D-lactate dehydrogenase Burkholderia multivorans Planktonic cellular aggregates Biofilms Exopolysaccharide Cystic fibrosis |
| title_short |
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formation |
| title_full |
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formation |
| title_fullStr |
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formation |
| title_full_unstemmed |
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formation |
| title_sort |
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formation |
| author |
Silva, Inês N. |
| author_facet |
Silva, Inês N. Ramires, Marcelo J. Azevedo, Lisa A. Guerreiro, Ana R. Tavares, Andreia C. Becker, Jörg D. Moreira, Leonilde M. |
| author_role |
author |
| author2 |
Ramires, Marcelo J. Azevedo, Lisa A. Guerreiro, Ana R. Tavares, Andreia C. Becker, Jörg D. Moreira, Leonilde M. |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
ARCA |
| dc.contributor.author.fl_str_mv |
Silva, Inês N. Ramires, Marcelo J. Azevedo, Lisa A. Guerreiro, Ana R. Tavares, Andreia C. Becker, Jörg D. Moreira, Leonilde M. |
| dc.subject.por.fl_str_mv |
LysR-family transcriptional regulator D-lactate dehydrogenase Burkholderia multivorans Planktonic cellular aggregates Biofilms Exopolysaccharide Cystic fibrosis |
| topic |
LysR-family transcriptional regulator D-lactate dehydrogenase Burkholderia multivorans Planktonic cellular aggregates Biofilms Exopolysaccharide Cystic fibrosis |
| description |
This paper version is the accepted manuscript posted online 21 July 2017. It has peer-review. This publication hasn't any creative commons license associated. The deposited article version contains attached the supplementary materials within the pdf. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-07 2017-07-01T00:00:00Z 2018-08-01T00:30:08Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/777 |
| url |
http://hdl.handle.net/10400.7/777 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivorans play a role in carbon overflow and in planktonic cellular aggregates formation Inês N. Silva, Marcelo J. Ramires, Lisa A. Azevedo, Ana R. Guerreiro, Andreia C. Tavares, Jörg D. Becker, and Leonilde M. Moreira Appl. Environ. Microbiol. AEM.01343-17; Accepted manuscript posted online 21 July 2017, doi:10.1128/AEM.01343-17 10.1128/AEM.01343-17 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
| eu_rights_str_mv |
embargoedAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
American Society for Microbiology |
| publisher.none.fl_str_mv |
American Society for Microbiology |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799130575682928640 |