Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/168743 |
Resumo: | © 2024. The Author(s). |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupiImmunology and Microbiology(all)ParasitologyInfectious Diseasesveterinary(all)SDG 3 - Good Health and Well-being© 2024. The Author(s).Onchocerca lupi is a zoonotic filarioid parasite of dogs and cats with widespread distribution. A specific non-invasive diagnostic assay for the detection of O. lupi infections remains unavailable. This study aimed to assess the accuracy, specificity, and sensitivity of an ELISA test designed using nine peptides from two O. lupi proteins. Sera (n = 54) collected from O. lupi infected dogs from endemic areas (Portugal and USA), alongside sera from dogs positive for Dirofilaria immitis, D. repens, Cercopithifilaria bainae, and Acanthocheilonema reconditum (n = 53) from a non-endemic area for O. lupi, as well as from helminth-free dogs (n = 60), were tested. The checkerboard titration method was applied for the optimization of peptide concentrations and conjugate anti-dog dilutions. Sensitivity, specificity, and optimal cut-off values were calculated using ROC curve analysis. All peptides reacted against sera of O. lupi, with no correlation between optic density (OD) values and microfilariae (mfs) loads. Sensitivity and specificity values ranging from 85.45 to 100%, and 88.89% to 100%, respectively, were recorded for all peptides examined, with 100% specificity and sensitivity observed for peptides 40_3, 40_5, 130_3, 120_3 and 40_1, 130_5, respectively. The maximum cut-off value was observed for peptides 40_5 (0.765) and 40_3 (0.708). Testing of sera from dogs positive for other filarioids resulted in lower OD values (up to 1.565) for peptides 40_3 and 40_5 when compared with O. lupi (up to 2.929). The availability of this assay will be of value in epidemiological studies of canine O. lupi infection in both endemic and non-endemic areas, and in assessing the risk for zoonotic transmission.Vector borne diseases and pathogens (VBD)Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)RUNLatrofa, Maria StefaniaLouzada-Flores, Viviane NollMaia, CarlaKelly, Maureen AVerocai, Guilherme GCantacessi, CinziaOtranto, Domenico2024-06-19T00:12:02Z2024-02-092024-02-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/168743eng2045-2322PURE: 83389720https://doi.org/10.1038/s41598-024-53759-winfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-10-14T01:38:20Zoai:run.unl.pt:10362/168743Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-10-14T01:38:20Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi |
title |
Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi |
spellingShingle |
Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi Latrofa, Maria Stefania Immunology and Microbiology(all) Parasitology Infectious Diseases veterinary(all) SDG 3 - Good Health and Well-being |
title_short |
Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi |
title_full |
Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi |
title_fullStr |
Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi |
title_full_unstemmed |
Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi |
title_sort |
Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi |
author |
Latrofa, Maria Stefania |
author_facet |
Latrofa, Maria Stefania Louzada-Flores, Viviane Noll Maia, Carla Kelly, Maureen A Verocai, Guilherme G Cantacessi, Cinzia Otranto, Domenico |
author_role |
author |
author2 |
Louzada-Flores, Viviane Noll Maia, Carla Kelly, Maureen A Verocai, Guilherme G Cantacessi, Cinzia Otranto, Domenico |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Vector borne diseases and pathogens (VBD) Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) RUN |
dc.contributor.author.fl_str_mv |
Latrofa, Maria Stefania Louzada-Flores, Viviane Noll Maia, Carla Kelly, Maureen A Verocai, Guilherme G Cantacessi, Cinzia Otranto, Domenico |
dc.subject.por.fl_str_mv |
Immunology and Microbiology(all) Parasitology Infectious Diseases veterinary(all) SDG 3 - Good Health and Well-being |
topic |
Immunology and Microbiology(all) Parasitology Infectious Diseases veterinary(all) SDG 3 - Good Health and Well-being |
description |
© 2024. The Author(s). |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-06-19T00:12:02Z 2024-02-09 2024-02-09T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/168743 |
url |
http://hdl.handle.net/10362/168743 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2045-2322 PURE: 83389720 https://doi.org/10.1038/s41598-024-53759-w |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817546127171387392 |