Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/26914 https://doi.org/10.1016/j.bios.2013.01.015 |
Resumo: | A novel biomimetic microsensor for measuring nitric oxide (NO) in the brain in vivo was developed. The sensor consists of hemin and functionalized multi-wall carbon nanotubes covalently attached to chitosan via the carbodiimide crosslinker EDC followed by chitosan electrodeposition on the surface of carbon fiber microelectrodes. Cyclic voltammetry supported direct electron transfer from the FeIII/FeII couple of hemin to the carbon surface at −0.370 V and −0.305 V vs. Ag/AgCl for cathodic and anodic peaks, respectively. Square wave voltammetry revealed a NO reduction peak at −0.762 V vs. Ag/AgCl that increased linearly with NO concentration between 0.25 and 1 μM. The average sensitivity of the microsensors was 1.72 nA/μM and the limit of detection was 25 nM. Oxygen and hydrogen peroxide reduction peaks were observed at −0.269 V and −0.332 V vs. Ag/AgCl, respectively and no response was observed for other relevant interferents, namely ascorbate, nitrite and dopamine. The microsensor was successfully applied to the measurement of exogenously applied NO in the rat brain in vivo. |
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Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brainNitric oxide microsensorHeminCarbon nanotubesChitosanIn vivo electrochemistryA novel biomimetic microsensor for measuring nitric oxide (NO) in the brain in vivo was developed. The sensor consists of hemin and functionalized multi-wall carbon nanotubes covalently attached to chitosan via the carbodiimide crosslinker EDC followed by chitosan electrodeposition on the surface of carbon fiber microelectrodes. Cyclic voltammetry supported direct electron transfer from the FeIII/FeII couple of hemin to the carbon surface at −0.370 V and −0.305 V vs. Ag/AgCl for cathodic and anodic peaks, respectively. Square wave voltammetry revealed a NO reduction peak at −0.762 V vs. Ag/AgCl that increased linearly with NO concentration between 0.25 and 1 μM. The average sensitivity of the microsensors was 1.72 nA/μM and the limit of detection was 25 nM. Oxygen and hydrogen peroxide reduction peaks were observed at −0.269 V and −0.332 V vs. Ag/AgCl, respectively and no response was observed for other relevant interferents, namely ascorbate, nitrite and dopamine. The microsensor was successfully applied to the measurement of exogenously applied NO in the rat brain in vivo.Elsevier2013-06-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/26914http://hdl.handle.net/10316/26914https://doi.org/10.1016/j.bios.2013.01.015engSANTOS, Ricardo M. [et al.] - Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain. "Biosensors and Bioelectronics". ISSN 0956-5663. Vol. 44 (2013) p. 152-1590956-5663http://www.sciencedirect.com/science/article/pii/S0956566313000171Santos, Ricardo M.Rodrigues, Marcelo S.Laranjinha, JoãoBarbosa, Rui M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-29T10:04:57Zoai:estudogeral.uc.pt:10316/26914Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:53:35.228863Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain |
title |
Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain |
spellingShingle |
Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain Santos, Ricardo M. Nitric oxide microsensor Hemin Carbon nanotubes Chitosan In vivo electrochemistry |
title_short |
Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain |
title_full |
Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain |
title_fullStr |
Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain |
title_full_unstemmed |
Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain |
title_sort |
Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain |
author |
Santos, Ricardo M. |
author_facet |
Santos, Ricardo M. Rodrigues, Marcelo S. Laranjinha, João Barbosa, Rui M. |
author_role |
author |
author2 |
Rodrigues, Marcelo S. Laranjinha, João Barbosa, Rui M. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Santos, Ricardo M. Rodrigues, Marcelo S. Laranjinha, João Barbosa, Rui M. |
dc.subject.por.fl_str_mv |
Nitric oxide microsensor Hemin Carbon nanotubes Chitosan In vivo electrochemistry |
topic |
Nitric oxide microsensor Hemin Carbon nanotubes Chitosan In vivo electrochemistry |
description |
A novel biomimetic microsensor for measuring nitric oxide (NO) in the brain in vivo was developed. The sensor consists of hemin and functionalized multi-wall carbon nanotubes covalently attached to chitosan via the carbodiimide crosslinker EDC followed by chitosan electrodeposition on the surface of carbon fiber microelectrodes. Cyclic voltammetry supported direct electron transfer from the FeIII/FeII couple of hemin to the carbon surface at −0.370 V and −0.305 V vs. Ag/AgCl for cathodic and anodic peaks, respectively. Square wave voltammetry revealed a NO reduction peak at −0.762 V vs. Ag/AgCl that increased linearly with NO concentration between 0.25 and 1 μM. The average sensitivity of the microsensors was 1.72 nA/μM and the limit of detection was 25 nM. Oxygen and hydrogen peroxide reduction peaks were observed at −0.269 V and −0.332 V vs. Ag/AgCl, respectively and no response was observed for other relevant interferents, namely ascorbate, nitrite and dopamine. The microsensor was successfully applied to the measurement of exogenously applied NO in the rat brain in vivo. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/26914 http://hdl.handle.net/10316/26914 https://doi.org/10.1016/j.bios.2013.01.015 |
url |
http://hdl.handle.net/10316/26914 https://doi.org/10.1016/j.bios.2013.01.015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
SANTOS, Ricardo M. [et al.] - Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain. "Biosensors and Bioelectronics". ISSN 0956-5663. Vol. 44 (2013) p. 152-159 0956-5663 http://www.sciencedirect.com/science/article/pii/S0956566313000171 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799133822345805824 |