Production of Aspergillus niger GH1 tannase using solid-state fermentation

Detalhes bibliográficos
Autor(a) principal: Cruz-Hernandez, M. A.
Data de Publicação: 2009
Outros Autores: Contreras, J. C., Lima, Nelson, Teixeira, J. A., Aguilar, Cristóbal N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/25949
Resumo: The production of tannase by Aspergillus niger GH1 in solid-state fermentation (SSF) was evaluated. Several concentrations of tannic acid (12.5, 25, 50 and 100 g/L) were tested in a model culture system. Column reactors were packed with polyurethane foam impregnated with liquid medium and inoculated with fungal spores. Tannase production was kinetically monitored by 48 h. Tannic acid uptake was spectrophotometrically measured and extracellular and intracellular tannase activities were assayed by HPLC-chromatography. Tannase activity increased with increasing concentration of tannic acid. Maximum extracellular and intracellular tannase activities (11.35 and 6.95 U/mL respectively) were recorded with 100 g/L of tannic acid. The substrate uptake was 100% at concentrations of 12.5, 25 and 50 g/L, while 74.4% was consumed in the presence of 100 g/L of tannic acid after 48 h of culture. These results suggest that high concentrations of tannins can be removed by SSF and tannase production can be reached in high levels.
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spelling Production of Aspergillus niger GH1 tannase using solid-state fermentationTannaseTannic acidSolid-state fermentationAspergillus niger GH1Science & TechnologyThe production of tannase by Aspergillus niger GH1 in solid-state fermentation (SSF) was evaluated. Several concentrations of tannic acid (12.5, 25, 50 and 100 g/L) were tested in a model culture system. Column reactors were packed with polyurethane foam impregnated with liquid medium and inoculated with fungal spores. Tannase production was kinetically monitored by 48 h. Tannic acid uptake was spectrophotometrically measured and extracellular and intracellular tannase activities were assayed by HPLC-chromatography. Tannase activity increased with increasing concentration of tannic acid. Maximum extracellular and intracellular tannase activities (11.35 and 6.95 U/mL respectively) were recorded with 100 g/L of tannic acid. The substrate uptake was 100% at concentrations of 12.5, 25 and 50 g/L, while 74.4% was consumed in the presence of 100 g/L of tannic acid after 48 h of culture. These results suggest that high concentrations of tannins can be removed by SSF and tannase production can be reached in high levels.The present work was performed as part of a cooperative agreement between the Universidad Autonoma de Coahuila (UAdeC, Mexico) and the Universidade do Minho (UM, Portugal) within a specific international exchange program (VALNATURA project, alfa network from European Union) undertaken at the Biological Engineering Department (UM, Portugal) M A Cruz-Hernandez thanks to Concejo nacional de Ciencia y Tecnologia (CONACYT) the fellowship to study the doctoral program at the Department of Biotechnology (UAdeC)Oriental Scientific Pub. Co.Universidade do MinhoCruz-Hernandez, M. A.Contreras, J. C.Lima, NelsonTeixeira, J. A.Aguilar, Cristóbal N.20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/25949eng0973-7510info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:53:26Zoai:repositorium.sdum.uminho.pt:1822/25949Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:52:48.887214Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Production of Aspergillus niger GH1 tannase using solid-state fermentation
title Production of Aspergillus niger GH1 tannase using solid-state fermentation
spellingShingle Production of Aspergillus niger GH1 tannase using solid-state fermentation
Cruz-Hernandez, M. A.
Tannase
Tannic acid
Solid-state fermentation
Aspergillus niger GH1
Science & Technology
title_short Production of Aspergillus niger GH1 tannase using solid-state fermentation
title_full Production of Aspergillus niger GH1 tannase using solid-state fermentation
title_fullStr Production of Aspergillus niger GH1 tannase using solid-state fermentation
title_full_unstemmed Production of Aspergillus niger GH1 tannase using solid-state fermentation
title_sort Production of Aspergillus niger GH1 tannase using solid-state fermentation
author Cruz-Hernandez, M. A.
author_facet Cruz-Hernandez, M. A.
Contreras, J. C.
Lima, Nelson
Teixeira, J. A.
Aguilar, Cristóbal N.
author_role author
author2 Contreras, J. C.
Lima, Nelson
Teixeira, J. A.
Aguilar, Cristóbal N.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Cruz-Hernandez, M. A.
Contreras, J. C.
Lima, Nelson
Teixeira, J. A.
Aguilar, Cristóbal N.
dc.subject.por.fl_str_mv Tannase
Tannic acid
Solid-state fermentation
Aspergillus niger GH1
Science & Technology
topic Tannase
Tannic acid
Solid-state fermentation
Aspergillus niger GH1
Science & Technology
description The production of tannase by Aspergillus niger GH1 in solid-state fermentation (SSF) was evaluated. Several concentrations of tannic acid (12.5, 25, 50 and 100 g/L) were tested in a model culture system. Column reactors were packed with polyurethane foam impregnated with liquid medium and inoculated with fungal spores. Tannase production was kinetically monitored by 48 h. Tannic acid uptake was spectrophotometrically measured and extracellular and intracellular tannase activities were assayed by HPLC-chromatography. Tannase activity increased with increasing concentration of tannic acid. Maximum extracellular and intracellular tannase activities (11.35 and 6.95 U/mL respectively) were recorded with 100 g/L of tannic acid. The substrate uptake was 100% at concentrations of 12.5, 25 and 50 g/L, while 74.4% was consumed in the presence of 100 g/L of tannic acid after 48 h of culture. These results suggest that high concentrations of tannins can be removed by SSF and tannase production can be reached in high levels.
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/25949
url http://hdl.handle.net/1822/25949
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0973-7510
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Oriental Scientific Pub. Co.
publisher.none.fl_str_mv Oriental Scientific Pub. Co.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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