Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers and irritants in the FSDC cell line
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5843 https://doi.org/10.1016/j.toxlet.2007.12.006 |
Resumo: | The development of non-animal methods for skin sensitization testing is an urgent challenge. Some of the most promising in vitro approaches are based on the analysis of phenotypical and functional modifications induced by sensitizers in dendritic cell models. In this work, we evaluated, for the first time, a fetal skin-derived dendritic cell line (FSDC) as a model to discriminate between sensitizers and irritants, through analysis of their effects on CD40 and CXCR4 protein expression. The chemicals concentrations were chosen based on a slight cytotoxicity effect (up to 15%). Protein levels were evaluated by Western blot and immunocytochemistry, after stimulation with the skin sensitizers 2,4-dinitrofluorobenzene (DNFB), 1,4-phenylenediamine (PPD) and nickel sulphate (NiSO4), the non-sensitizer 2,4-dichloronitrobenzene (DCNB), and the irritants sodium dodecyl sulphate (SDS) and benzalkonium chloride (BC). All sensitizers tested increased CD40 and CXCR4 levels. In contrast, irritants decreased both proteins levels, with a more pronounced effect on CXCR4. In agreement with these results, dendritic cells derived from human peripheral blood monocytes-derived dendritic cells (MoDC) showed a similar response pattern to the skin sensitizer and irritant tested, PPD and SDS, respectively. In conclusion, evaluation of CD40 and CXCR4 proteins in chemical-treated FSDC may represent a useful tool in a future in vitro test for sensitizing assessment. |
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Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers and irritants in the FSDC cell lineIn vitro sensitization testFSDC cell lineCXCR4CD40The development of non-animal methods for skin sensitization testing is an urgent challenge. Some of the most promising in vitro approaches are based on the analysis of phenotypical and functional modifications induced by sensitizers in dendritic cell models. In this work, we evaluated, for the first time, a fetal skin-derived dendritic cell line (FSDC) as a model to discriminate between sensitizers and irritants, through analysis of their effects on CD40 and CXCR4 protein expression. The chemicals concentrations were chosen based on a slight cytotoxicity effect (up to 15%). Protein levels were evaluated by Western blot and immunocytochemistry, after stimulation with the skin sensitizers 2,4-dinitrofluorobenzene (DNFB), 1,4-phenylenediamine (PPD) and nickel sulphate (NiSO4), the non-sensitizer 2,4-dichloronitrobenzene (DCNB), and the irritants sodium dodecyl sulphate (SDS) and benzalkonium chloride (BC). All sensitizers tested increased CD40 and CXCR4 levels. In contrast, irritants decreased both proteins levels, with a more pronounced effect on CXCR4. In agreement with these results, dendritic cells derived from human peripheral blood monocytes-derived dendritic cells (MoDC) showed a similar response pattern to the skin sensitizer and irritant tested, PPD and SDS, respectively. In conclusion, evaluation of CD40 and CXCR4 proteins in chemical-treated FSDC may represent a useful tool in a future in vitro test for sensitizing assessment.2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5843http://hdl.handle.net/10316/5843https://doi.org/10.1016/j.toxlet.2007.12.006engToxicology Letters. 177:1 (2008) 74-82Neves, Bruno MiguelCruz, Maria TeresaFrancisco, VeraGonçalo, MargaridaFigueiredo, AméricoDuarte, Carlos B.Lopes, Maria Celesteinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T03:34:33Zoai:estudogeral.uc.pt:10316/5843Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:25.461567Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers and irritants in the FSDC cell line |
title |
Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers and irritants in the FSDC cell line |
spellingShingle |
Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers and irritants in the FSDC cell line Neves, Bruno Miguel In vitro sensitization test FSDC cell line CXCR4 CD40 |
title_short |
Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers and irritants in the FSDC cell line |
title_full |
Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers and irritants in the FSDC cell line |
title_fullStr |
Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers and irritants in the FSDC cell line |
title_full_unstemmed |
Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers and irritants in the FSDC cell line |
title_sort |
Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers and irritants in the FSDC cell line |
author |
Neves, Bruno Miguel |
author_facet |
Neves, Bruno Miguel Cruz, Maria Teresa Francisco, Vera Gonçalo, Margarida Figueiredo, Américo Duarte, Carlos B. Lopes, Maria Celeste |
author_role |
author |
author2 |
Cruz, Maria Teresa Francisco, Vera Gonçalo, Margarida Figueiredo, Américo Duarte, Carlos B. Lopes, Maria Celeste |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Neves, Bruno Miguel Cruz, Maria Teresa Francisco, Vera Gonçalo, Margarida Figueiredo, Américo Duarte, Carlos B. Lopes, Maria Celeste |
dc.subject.por.fl_str_mv |
In vitro sensitization test FSDC cell line CXCR4 CD40 |
topic |
In vitro sensitization test FSDC cell line CXCR4 CD40 |
description |
The development of non-animal methods for skin sensitization testing is an urgent challenge. Some of the most promising in vitro approaches are based on the analysis of phenotypical and functional modifications induced by sensitizers in dendritic cell models. In this work, we evaluated, for the first time, a fetal skin-derived dendritic cell line (FSDC) as a model to discriminate between sensitizers and irritants, through analysis of their effects on CD40 and CXCR4 protein expression. The chemicals concentrations were chosen based on a slight cytotoxicity effect (up to 15%). Protein levels were evaluated by Western blot and immunocytochemistry, after stimulation with the skin sensitizers 2,4-dinitrofluorobenzene (DNFB), 1,4-phenylenediamine (PPD) and nickel sulphate (NiSO4), the non-sensitizer 2,4-dichloronitrobenzene (DCNB), and the irritants sodium dodecyl sulphate (SDS) and benzalkonium chloride (BC). All sensitizers tested increased CD40 and CXCR4 levels. In contrast, irritants decreased both proteins levels, with a more pronounced effect on CXCR4. In agreement with these results, dendritic cells derived from human peripheral blood monocytes-derived dendritic cells (MoDC) showed a similar response pattern to the skin sensitizer and irritant tested, PPD and SDS, respectively. In conclusion, evaluation of CD40 and CXCR4 proteins in chemical-treated FSDC may represent a useful tool in a future in vitro test for sensitizing assessment. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5843 http://hdl.handle.net/10316/5843 https://doi.org/10.1016/j.toxlet.2007.12.006 |
url |
http://hdl.handle.net/10316/5843 https://doi.org/10.1016/j.toxlet.2007.12.006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicology Letters. 177:1 (2008) 74-82 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133751207264256 |