Typing of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein gene

Detalhes bibliográficos
Autor(a) principal: Amin, H. A.
Data de Publicação: 2006
Outros Autores: Fonseca, Filomena, Santos, C., Nolasco, Gustavo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/6250
Resumo: The capsid protein gene of three Egyptian CTV isolates from two locations was amplified by immunocapture RT-PCR and analysed by single stranded conformation polymorphism and sequencing. The CTV isolates studied did not differ significantly in sequence composition and each isolate consisted of very similar haplotypes. Comparison with reference sequences from isolates elsewhere in the world showed that these haplotypes clustered very close to the severe strain T3 from Florida causing quick decline and stem pitting. Analysis of the deduced amino acid sequence showed the epitope characteristic of reactivity with the MCA13 antibody. Sequence comparison with the sequence of an Egyptian isolate (Qaha) available in the Genbank showed a distance of about 8%, suggesting that it had a different origin.
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spelling Typing of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein geneThe capsid protein gene of three Egyptian CTV isolates from two locations was amplified by immunocapture RT-PCR and analysed by single stranded conformation polymorphism and sequencing. The CTV isolates studied did not differ significantly in sequence composition and each isolate consisted of very similar haplotypes. Comparison with reference sequences from isolates elsewhere in the world showed that these haplotypes clustered very close to the severe strain T3 from Florida causing quick decline and stem pitting. Analysis of the deduced amino acid sequence showed the epitope characteristic of reactivity with the MCA13 antibody. Sequence comparison with the sequence of an Egyptian isolate (Qaha) available in the Genbank showed a distance of about 8%, suggesting that it had a different origin.SapientiaAmin, H. A.Fonseca, FilomenaSantos, C.Nolasco, Gustavo2015-06-15T14:13:48Z20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/6250eng0031-9465AUT: GNO00324; FFO00315;info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:17:27Zoai:sapientia.ualg.pt:10400.1/6250Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:59:03.377599Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Typing of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein gene
title Typing of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein gene
spellingShingle Typing of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein gene
Amin, H. A.
title_short Typing of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein gene
title_full Typing of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein gene
title_fullStr Typing of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein gene
title_full_unstemmed Typing of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein gene
title_sort Typing of Egyptian Citrus tristeza virus (CTV) isolates based on the capsid protein gene
author Amin, H. A.
author_facet Amin, H. A.
Fonseca, Filomena
Santos, C.
Nolasco, Gustavo
author_role author
author2 Fonseca, Filomena
Santos, C.
Nolasco, Gustavo
author2_role author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Amin, H. A.
Fonseca, Filomena
Santos, C.
Nolasco, Gustavo
description The capsid protein gene of three Egyptian CTV isolates from two locations was amplified by immunocapture RT-PCR and analysed by single stranded conformation polymorphism and sequencing. The CTV isolates studied did not differ significantly in sequence composition and each isolate consisted of very similar haplotypes. Comparison with reference sequences from isolates elsewhere in the world showed that these haplotypes clustered very close to the severe strain T3 from Florida causing quick decline and stem pitting. Analysis of the deduced amino acid sequence showed the epitope characteristic of reactivity with the MCA13 antibody. Sequence comparison with the sequence of an Egyptian isolate (Qaha) available in the Genbank showed a distance of about 8%, suggesting that it had a different origin.
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
2015-06-15T14:13:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/6250
url http://hdl.handle.net/10400.1/6250
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0031-9465
AUT: GNO00324; FFO00315;
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