Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788

Detalhes bibliográficos
Autor(a) principal: Plácido, Alexandra
Data de Publicação: 2019
Outros Autores: Pereira, Clara, Barroso, M. Fátima, de-los-Santos-Álvarez, Noemí, Delerue-Matos, Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/16105
Resumo: This work received financial support from the European Union (FEDER funds through COMPETE), National Funds (FCT, Fundação para a Ciência e a Tecnologia) through project UID/QUI/50006/2013. A.P. and M.F.B. are grateful to FCT grants SFRH/BD/97995/2013 and SFRH/BPD/78845/2011, financed by POPH–QREN–Tipologia 4.1–Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. C.P. thanks FCT for the FCT Investigator contract IF/01080/2015.
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spelling Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788Core-shell Fe3O4@Au magnetic nanoparticlesGTS 40-3-2 MON89788Electrochemical genoassaySimultaneous detectionThis work received financial support from the European Union (FEDER funds through COMPETE), National Funds (FCT, Fundação para a Ciência e a Tecnologia) through project UID/QUI/50006/2013. A.P. and M.F.B. are grateful to FCT grants SFRH/BD/97995/2013 and SFRH/BPD/78845/2011, financed by POPH–QREN–Tipologia 4.1–Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. C.P. thanks FCT for the FCT Investigator contract IF/01080/2015.Development of expeditious analytical methods for the detection of genetically modified organisms (GMOs) is increasingly necessary, not only to verify compliance with labelling, but also to help industry to efficiently control the reception of raw materials. On the basis of this, a disposable electrochemical magnetogenoassay is proposed for simultaneous detection of two Roundup Ready (RR) soybean lines GTS 40-3-2 and MON89788, using gold-coated magnetic nanoparticles (Fe3O4@Au) as nanosupport. To perform this magnetogenoassay, a sandwich-type hybridization assay was used with different enzymatic labelling systems (fluorescein isothiocyanate and digoxigenin) and dual screen-printed carbon electrodes (SPdCEs), which allowed the simultaneous readout of each target. A linear relationship ranging from 0.1 to 2.5 nM and from 0.1 to 1.0 nM was achieved for GTS 40-3-2 and MON89788 events, respectively, and both assays showed a similar detection limit of about 0.1 nM. Furthermore, a good performance in terms of precision and selectivity was achieved. The proposed approach is a step forward for event-specific multiplex detection.ElsevierRepositório Científico do Instituto Politécnico do PortoPlácido, AlexandraPereira, ClaraBarroso, M. Fátimade-los-Santos-Álvarez, NoemíDelerue-Matos, Cristina2020-07-10T14:31:45Z2019-032019-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/16105eng10.1016/j.snb.2018.12.009info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:02:10Zoai:recipp.ipp.pt:10400.22/16105Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:35:49.103579Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788
title Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788
spellingShingle Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788
Plácido, Alexandra
Core-shell Fe3O4@Au magnetic nanoparticles
GTS 40-3-2 MON89788
Electrochemical genoassay
Simultaneous detection
title_short Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788
title_full Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788
title_fullStr Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788
title_full_unstemmed Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788
title_sort Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788
author Plácido, Alexandra
author_facet Plácido, Alexandra
Pereira, Clara
Barroso, M. Fátima
de-los-Santos-Álvarez, Noemí
Delerue-Matos, Cristina
author_role author
author2 Pereira, Clara
Barroso, M. Fátima
de-los-Santos-Álvarez, Noemí
Delerue-Matos, Cristina
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Plácido, Alexandra
Pereira, Clara
Barroso, M. Fátima
de-los-Santos-Álvarez, Noemí
Delerue-Matos, Cristina
dc.subject.por.fl_str_mv Core-shell Fe3O4@Au magnetic nanoparticles
GTS 40-3-2 MON89788
Electrochemical genoassay
Simultaneous detection
topic Core-shell Fe3O4@Au magnetic nanoparticles
GTS 40-3-2 MON89788
Electrochemical genoassay
Simultaneous detection
description This work received financial support from the European Union (FEDER funds through COMPETE), National Funds (FCT, Fundação para a Ciência e a Tecnologia) through project UID/QUI/50006/2013. A.P. and M.F.B. are grateful to FCT grants SFRH/BD/97995/2013 and SFRH/BPD/78845/2011, financed by POPH–QREN–Tipologia 4.1–Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. C.P. thanks FCT for the FCT Investigator contract IF/01080/2015.
publishDate 2019
dc.date.none.fl_str_mv 2019-03
2019-03-01T00:00:00Z
2020-07-10T14:31:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/16105
url http://hdl.handle.net/10400.22/16105
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.snb.2018.12.009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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