Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/8481 |
Resumo: | This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics. |
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Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ CarcinogenesisHuman PapillomavirusHPVHPV16K14HPV16CarcinogenesisLineageVariantInfecções Sexualmente TransmissíveisThis article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics.The study of ()-induced carcinogenesis uses multiple in vivo mouse models, one of which relies on the cytokeratin 14 gene promoter to drive the expression of all HPV early oncogenes. This study aimed to determine the HPV16 variant and sublineage present in the K14HPV16 mouse model. This information can be considered of great importance to further enhance this K14HPV16 model as an essential research tool and optimize its use for basic and translational studies. Our study evaluated HPV DNA from 17 samples isolated from 4 animals, both wild-type (n = 2) and HPV16-transgenic mice (n = 2). Total DNA was extracted from tissues and the detection of HPV16 was performed using a qPCR multiplex. HPV16-positive samples were subsequently whole-genome sequenced by next-generation sequencing techniques. The phylogenetic positioning clearly shows K14HPV16 samples clustering together in the sub-lineage A1 (NC001526.4). A comparative genome analysis of K14HPV16 samples revealed three mutations to the human papillomaviruses type 16 sublineage A1 representative strain. Knowledge of the HPV 16 variant is fundamental, and these findings will allow the rational use of this animal model to explore the role of the A1 sublineage in HPV-driven cancer.This work is a result of Fundação para a Ciência e a Tecnologia (FCT); and also funded by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020); from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020—Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Por tugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT). This work was also supported by Fundos FEDER through the Programa Operacional Factores de Competitividade—COMPETE and by Fundos Nacionais through the Fundação para a Ciência e a Tec nologia within the scope of the project UID/BIM/00009/2019 (Centre for Toxicogenomics and Human Health-ToxOmics); and from LA/P/0045/2020 (ALiCE), UIDB/00511/2020 and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC); 2SMART (NORTE-01-0145- FEDER-000054), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).Repositório Científico do Instituto Nacional de SaúdeCochicho, DanielaNunes, AlexandraGomes, João PauloMartins, LuísCunha, MárioMedeiros-Fonseca, BeatrizOliveira, PaulaBastos, Margarida M.S.M.Medeiros, RuiMendonça, JoanaVieira, LuisGil da Costa, Rui M.Felix, Ana2023-01-31T12:45:59Z2022-10-152022-10-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8481engInt J Mol Sci. 2022 Oct 15;23(20):12371. doi: 10.3390/ijms2320123711661-659610.3390/ijms232012371info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:35Zoai:repositorio.insa.pt:10400.18/8481Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:06.475280Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis |
| title |
Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis |
| spellingShingle |
Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis Cochicho, Daniela Human Papillomavirus HPV HPV16 K14HPV16 Carcinogenesis Lineage Variant Infecções Sexualmente Transmissíveis |
| title_short |
Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis |
| title_full |
Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis |
| title_fullStr |
Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis |
| title_full_unstemmed |
Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis |
| title_sort |
Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis |
| author |
Cochicho, Daniela |
| author_facet |
Cochicho, Daniela Nunes, Alexandra Gomes, João Paulo Martins, Luís Cunha, Mário Medeiros-Fonseca, Beatriz Oliveira, Paula Bastos, Margarida M.S.M. Medeiros, Rui Mendonça, Joana Vieira, Luis Gil da Costa, Rui M. Felix, Ana |
| author_role |
author |
| author2 |
Nunes, Alexandra Gomes, João Paulo Martins, Luís Cunha, Mário Medeiros-Fonseca, Beatriz Oliveira, Paula Bastos, Margarida M.S.M. Medeiros, Rui Mendonça, Joana Vieira, Luis Gil da Costa, Rui M. Felix, Ana |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Cochicho, Daniela Nunes, Alexandra Gomes, João Paulo Martins, Luís Cunha, Mário Medeiros-Fonseca, Beatriz Oliveira, Paula Bastos, Margarida M.S.M. Medeiros, Rui Mendonça, Joana Vieira, Luis Gil da Costa, Rui M. Felix, Ana |
| dc.subject.por.fl_str_mv |
Human Papillomavirus HPV HPV16 K14HPV16 Carcinogenesis Lineage Variant Infecções Sexualmente Transmissíveis |
| topic |
Human Papillomavirus HPV HPV16 K14HPV16 Carcinogenesis Lineage Variant Infecções Sexualmente Transmissíveis |
| description |
This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-10-15 2022-10-15T00:00:00Z 2023-01-31T12:45:59Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10400.18/8481 |
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http://hdl.handle.net/10400.18/8481 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Int J Mol Sci. 2022 Oct 15;23(20):12371. doi: 10.3390/ijms232012371 1661-6596 10.3390/ijms232012371 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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