Development of a New Amperometric Biosensor for Lactose Determination

Detalhes bibliográficos
Autor(a) principal: Lourenço,R.J.M.
Data de Publicação: 2003
Outros Autores: Serralheiro,M.L.M., Rebelo,M.J.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042003000200005
Resumo: A new amperometric biosensor for lactose determinaion in raw milk was developed through the simultaneous immobilization of β-galactosidase and galactose oxidase on a derivatised polyethersulphone membrane. β-galactosidase catalyses the hydrolysis of lactose into galactose and glucose and galactose oxidase catalyses the oxidation of galactose into galactonic acid and H2O2. The membranes with the two immobilized enzymes were then used in an amperometric sensor, by oxidation of the H2O2 formed, at a Pt electrode of an Universal Sensors electrode base system. The sensitivity and the reproducibility of the biosensor thus formed were found to be 6.81 and 0.72 nA.M-1, respectively. Biosensors were found to be stable for 20 days.
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spelling Development of a New Amperometric Biosensor for Lactose Determinationbiosensorlactoseamperometricstabilization<FONT FACE=Symbol>b</font>-galactosidasegalactose oxidaseA new amperometric biosensor for lactose determinaion in raw milk was developed through the simultaneous immobilization of β-galactosidase and galactose oxidase on a derivatised polyethersulphone membrane. β-galactosidase catalyses the hydrolysis of lactose into galactose and glucose and galactose oxidase catalyses the oxidation of galactose into galactonic acid and H2O2. The membranes with the two immobilized enzymes were then used in an amperometric sensor, by oxidation of the H2O2 formed, at a Pt electrode of an Universal Sensors electrode base system. The sensitivity and the reproducibility of the biosensor thus formed were found to be 6.81 and 0.72 nA.M-1, respectively. Biosensors were found to be stable for 20 days.Sociedade Portuguesa de Electroquímica2003-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042003000200005Portugaliae Electrochimica Acta v.21 n.2 2003reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042003000200005Lourenço,R.J.M.Serralheiro,M.L.M.Rebelo,M.J.F.info:eu-repo/semantics/openAccess2024-02-06T17:06:41Zoai:scielo:S0872-19042003000200005Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:19:56.072162Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of a New Amperometric Biosensor for Lactose Determination
title Development of a New Amperometric Biosensor for Lactose Determination
spellingShingle Development of a New Amperometric Biosensor for Lactose Determination
Lourenço,R.J.M.
biosensor
lactose
amperometric
stabilization
<FONT FACE=Symbol>b</font>-galactosidase
galactose oxidase
title_short Development of a New Amperometric Biosensor for Lactose Determination
title_full Development of a New Amperometric Biosensor for Lactose Determination
title_fullStr Development of a New Amperometric Biosensor for Lactose Determination
title_full_unstemmed Development of a New Amperometric Biosensor for Lactose Determination
title_sort Development of a New Amperometric Biosensor for Lactose Determination
author Lourenço,R.J.M.
author_facet Lourenço,R.J.M.
Serralheiro,M.L.M.
Rebelo,M.J.F.
author_role author
author2 Serralheiro,M.L.M.
Rebelo,M.J.F.
author2_role author
author
dc.contributor.author.fl_str_mv Lourenço,R.J.M.
Serralheiro,M.L.M.
Rebelo,M.J.F.
dc.subject.por.fl_str_mv biosensor
lactose
amperometric
stabilization
<FONT FACE=Symbol>b</font>-galactosidase
galactose oxidase
topic biosensor
lactose
amperometric
stabilization
<FONT FACE=Symbol>b</font>-galactosidase
galactose oxidase
description A new amperometric biosensor for lactose determinaion in raw milk was developed through the simultaneous immobilization of β-galactosidase and galactose oxidase on a derivatised polyethersulphone membrane. β-galactosidase catalyses the hydrolysis of lactose into galactose and glucose and galactose oxidase catalyses the oxidation of galactose into galactonic acid and H2O2. The membranes with the two immobilized enzymes were then used in an amperometric sensor, by oxidation of the H2O2 formed, at a Pt electrode of an Universal Sensors electrode base system. The sensitivity and the reproducibility of the biosensor thus formed were found to be 6.81 and 0.72 nA.M-1, respectively. Biosensors were found to be stable for 20 days.
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042003000200005
url http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042003000200005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042003000200005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Portuguesa de Electroquímica
publisher.none.fl_str_mv Sociedade Portuguesa de Electroquímica
dc.source.none.fl_str_mv Portugaliae Electrochimica Acta v.21 n.2 2003
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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