b-GALACTOSIDASE IMMOBILIZATION ON CONTROLLED PORE SILICA

• Autor(a) principal: Trevisan,H. C.
• Data de Publicação: 1997
• Outros Autores: Bergamo,E. P., Contiero,J., Hojo,O., Monti,R.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Chemical Engineering
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400003
• topic: <FONT FACE=Symbol>b</font> -galactosidase; immobilization; whey; lactose hydrolysis

The immobilization of <FONT FACE="Symbol">b</font> -galactosidase from Kluyveromyces fragilis on controlled pore silica was investigated. Immobilization was performed on amino silica activated with glutaraldehyde and the product was applied to the hydrolysis of lactose of whey. The behaviors of the soluble and immobilized enzyme were compared by using whey and a lactose solution as the substrate. With the aim of optimizing the method, parameters such as the amount of glutaraldehyde and the size of the particles were evaluated by comparing activities and stabilities on batch and continuously fluidized bed reactors