Identification of novel genes for bitter taste receptors in sheep (Ovis aries)

Detalhes bibliográficos
Autor(a) principal: Ferreira, A. M.
Data de Publicação: 2013
Outros Autores: Araújo, S. S., Sales-Baptista, E., Almeida, A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/7176
Resumo: Articles in International Journals
id RCAP_75dcbff8836ab798930254640a5aff93
oai_identifier_str oai:www.repository.utl.pt:10400.5/7176
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Identification of novel genes for bitter taste receptors in sheep (Ovis aries)Bitter tasteTaste receptorsT2RSheepIngestive behaviorArticles in International JournalsGenetic studies on taste sensitivity, and bitter taste receptors (T2R) in particular, are an essential tool to understand ingestive behavior and its relation to variations of nutritional status occurring in ruminants. In the present study, we conducted a data-mining search to identify T2R candidates in sheep by comparison with the described T2R in cattle and using recently available ovine genome. In sheep, we identified eight orthologs of cattle genes: T2R16, T2R10B, T2R12, T2R3, T2R4, T2R67, T2R13 and T2R5. The in silico predicted genes were then confirmed by PCR and DNA sequencing. The sequencing results showed a 99% to 100% similarity with the in silico predicted sequence. Moreover, we address the chromosomal distribution and compare, in homology and phylogenetic terms, the obtained genes with the known T2R in human, mouse, dog, cattle, horse and pig. The eight novel genes identified map either to ovine chromosome 3 or 4. The phylogenetic data suggest a clustering by receptor type rather than by species for some of the receptors. From the species analyzed, we observed a clear proximity between the two ruminant species, sheep and cattle, in contrast with lower similarities obtained for the comparison of sheep with other mammals. Although further studies are needed to identify the complete T2R repertoire in domestic sheep, our data represent a first step for genetic studies on this field.The Animal ConsortiumRepositório da Universidade de LisboaFerreira, A. M.Araújo, S. S.Sales-Baptista, E.Almeida, A. M.2014-09-19T09:44:23Z2013-042013-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/7176engFerreira, A.M., Araújo, S.S., Sales-Baptista, E., Almeida, A.M. (2013). Identification of novel genes for bitter taste receptors in sheep (Ovis aries). Animal, 7(4), 547-554. doi: 10.1017/S17517311120020301751-732X (online)10.1017/S1751731112002030info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:37:55Zoai:www.repository.utl.pt:10400.5/7176Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:54:22.151775Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Identification of novel genes for bitter taste receptors in sheep (Ovis aries)
title Identification of novel genes for bitter taste receptors in sheep (Ovis aries)
spellingShingle Identification of novel genes for bitter taste receptors in sheep (Ovis aries)
Ferreira, A. M.
Bitter taste
Taste receptors
T2R
Sheep
Ingestive behavior
title_short Identification of novel genes for bitter taste receptors in sheep (Ovis aries)
title_full Identification of novel genes for bitter taste receptors in sheep (Ovis aries)
title_fullStr Identification of novel genes for bitter taste receptors in sheep (Ovis aries)
title_full_unstemmed Identification of novel genes for bitter taste receptors in sheep (Ovis aries)
title_sort Identification of novel genes for bitter taste receptors in sheep (Ovis aries)
author Ferreira, A. M.
author_facet Ferreira, A. M.
Araújo, S. S.
Sales-Baptista, E.
Almeida, A. M.
author_role author
author2 Araújo, S. S.
Sales-Baptista, E.
Almeida, A. M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Ferreira, A. M.
Araújo, S. S.
Sales-Baptista, E.
Almeida, A. M.
dc.subject.por.fl_str_mv Bitter taste
Taste receptors
T2R
Sheep
Ingestive behavior
topic Bitter taste
Taste receptors
T2R
Sheep
Ingestive behavior
description Articles in International Journals
publishDate 2013
dc.date.none.fl_str_mv 2013-04
2013-04-01T00:00:00Z
2014-09-19T09:44:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/7176
url http://hdl.handle.net/10400.5/7176
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ferreira, A.M., Araújo, S.S., Sales-Baptista, E., Almeida, A.M. (2013). Identification of novel genes for bitter taste receptors in sheep (Ovis aries). Animal, 7(4), 547-554. doi: 10.1017/S1751731112002030
1751-732X (online)
10.1017/S1751731112002030
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Animal Consortium
publisher.none.fl_str_mv The Animal Consortium
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131019537809408

Registros relacionados