On the bioactivity of Echinacea purpurea extracts to modulate the production of inflammatory mediators
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/81148 |
Resumo: | Inflammatory diseases are the focus of several clinical studies, due to limitations and serious side effects of available therapies. Plant-based drugs (e.g., salicylic acid, morphine) have become landmarks in the pharmaceutical field. Therefore, we investigated the immunomodulatory effects of flowers, leaves, and roots from <i>Echinacea purpurea</i>. Ethanolic (EE) and dichloromethanolic extracts (DE) were obtained using the Accelerated Solvent Extractor and aqueous extracts (AE) were prepared under stirring. Their chemical fingerprint was evaluated by liquid chromatography–high resolution mass spectrometry (LC-HRMS). The pro- and anti-inflammatory effects, as well as the reduction in intracellular reactive oxygen and nitrogen species (ROS/RNS), of the different extracts were evaluated using non-stimulated and lipopolysaccharide-stimulated macrophages. Interestingly, AE were able to stimulate macrophages to produce pro-inflammatory cytokines (tumor necrosis factor -TNF-α, interleukin -IL-1β, and IL-6), and to generate ROS/RNS. Conversely, under an inflammatory scenario, all extracts reduced the amount of pro-inflammatory mediators. DE, alkylamides-enriched extracts, showed the strongest anti-inflammatory activity. Moreover, <i>E. purpurea</i> extracts demonstrated generally a more robust anti-inflammatory activity than clinically used anti-inflammatory drugs (dexamethasone, diclofenac, salicylic acid, and celecoxib). Therefore, <i>E. purpurea</i> extracts may be used to develop new effective therapeutic formulations for disorders in which the immune system is either overactive or impaired. |
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On the bioactivity of Echinacea purpurea extracts to modulate the production of inflammatory mediatorsEchinacea purpurea extractsinflammationpro-inflammatory cytokinesreactive oxygen and nitrogen speciesmacrophagesalkylamidesphenolsacidsphenols/acidsScience & TechnologyInflammatory diseases are the focus of several clinical studies, due to limitations and serious side effects of available therapies. Plant-based drugs (e.g., salicylic acid, morphine) have become landmarks in the pharmaceutical field. Therefore, we investigated the immunomodulatory effects of flowers, leaves, and roots from <i>Echinacea purpurea</i>. Ethanolic (EE) and dichloromethanolic extracts (DE) were obtained using the Accelerated Solvent Extractor and aqueous extracts (AE) were prepared under stirring. Their chemical fingerprint was evaluated by liquid chromatography–high resolution mass spectrometry (LC-HRMS). The pro- and anti-inflammatory effects, as well as the reduction in intracellular reactive oxygen and nitrogen species (ROS/RNS), of the different extracts were evaluated using non-stimulated and lipopolysaccharide-stimulated macrophages. Interestingly, AE were able to stimulate macrophages to produce pro-inflammatory cytokines (tumor necrosis factor -TNF-α, interleukin -IL-1β, and IL-6), and to generate ROS/RNS. Conversely, under an inflammatory scenario, all extracts reduced the amount of pro-inflammatory mediators. DE, alkylamides-enriched extracts, showed the strongest anti-inflammatory activity. Moreover, <i>E. purpurea</i> extracts demonstrated generally a more robust anti-inflammatory activity than clinically used anti-inflammatory drugs (dexamethasone, diclofenac, salicylic acid, and celecoxib). Therefore, <i>E. purpurea</i> extracts may be used to develop new effective therapeutic formulations for disorders in which the immune system is either overactive or impaired.This research was funded by the Fundação para a Ciência e a Tecnologia (FCT) to the PhD grant of SFV (PD/BD/135246/2017 and COVID/BD/152012/2021) and the projects PATH (PD/00169/2013), FROnTHERA (NORTE-01-0145-FEDER-000023), Cells4_IDs (PTDC/BTM-SAL/28882/2017), and the NORTE 2020 Structured Project, co-funded by Norte2020 (NORTE-01-0145-FEDER-000021). This research was also partially supported by national funds by FCT through the projects UIDB/04423/2020 and UIDP/04423/2020 (Group of Natural Products and Medicinal Chemistry-CIIMAR) and ERDF, through the COMPETE—POFC program in the framework of the program PT2020.Multidisciplinary Digital Publishing InstituteUniversidade do MinhoVieira, Sara F.Gonçalves, Virgínia M. F.Llaguno, Carmen P.Macías, FelipeTiritan, Maria ElizabethReis, R. L.Ferreira, Helena Susana Costa MachadoNeves, N. M.2022-11-062022-11-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/81148engVieira, S.F.; Gonçalves, V.M.F.; Llaguno, C.P.; Macías, F.; Tiritan, M.E.; Reis, R.L.; Ferreira, H.; Neves, N.M. On the Bioactivity of Echinacea purpurea Extracts to Modulate the Production of Inflammatory Mediators. Int. J. Mol. Sci. 2022, 23, 13616. https://doi.org/10.3390/ijms2321136161661-65961661-659610.3390/ijms23211361636362404https://www.mdpi.com/1422-0067/23/21/13616info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-22T01:15:56Zoai:repositorium.sdum.uminho.pt:1822/81148Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:50:46.984554Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
On the bioactivity of Echinacea purpurea extracts to modulate the production of inflammatory mediators |
title |
On the bioactivity of Echinacea purpurea extracts to modulate the production of inflammatory mediators |
spellingShingle |
On the bioactivity of Echinacea purpurea extracts to modulate the production of inflammatory mediators Vieira, Sara F. Echinacea purpurea extracts inflammation pro-inflammatory cytokines reactive oxygen and nitrogen species macrophages alkylamides phenols acids phenols/acids Science & Technology |
title_short |
On the bioactivity of Echinacea purpurea extracts to modulate the production of inflammatory mediators |
title_full |
On the bioactivity of Echinacea purpurea extracts to modulate the production of inflammatory mediators |
title_fullStr |
On the bioactivity of Echinacea purpurea extracts to modulate the production of inflammatory mediators |
title_full_unstemmed |
On the bioactivity of Echinacea purpurea extracts to modulate the production of inflammatory mediators |
title_sort |
On the bioactivity of Echinacea purpurea extracts to modulate the production of inflammatory mediators |
author |
Vieira, Sara F. |
author_facet |
Vieira, Sara F. Gonçalves, Virgínia M. F. Llaguno, Carmen P. Macías, Felipe Tiritan, Maria Elizabeth Reis, R. L. Ferreira, Helena Susana Costa Machado Neves, N. M. |
author_role |
author |
author2 |
Gonçalves, Virgínia M. F. Llaguno, Carmen P. Macías, Felipe Tiritan, Maria Elizabeth Reis, R. L. Ferreira, Helena Susana Costa Machado Neves, N. M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Vieira, Sara F. Gonçalves, Virgínia M. F. Llaguno, Carmen P. Macías, Felipe Tiritan, Maria Elizabeth Reis, R. L. Ferreira, Helena Susana Costa Machado Neves, N. M. |
dc.subject.por.fl_str_mv |
Echinacea purpurea extracts inflammation pro-inflammatory cytokines reactive oxygen and nitrogen species macrophages alkylamides phenols acids phenols/acids Science & Technology |
topic |
Echinacea purpurea extracts inflammation pro-inflammatory cytokines reactive oxygen and nitrogen species macrophages alkylamides phenols acids phenols/acids Science & Technology |
description |
Inflammatory diseases are the focus of several clinical studies, due to limitations and serious side effects of available therapies. Plant-based drugs (e.g., salicylic acid, morphine) have become landmarks in the pharmaceutical field. Therefore, we investigated the immunomodulatory effects of flowers, leaves, and roots from <i>Echinacea purpurea</i>. Ethanolic (EE) and dichloromethanolic extracts (DE) were obtained using the Accelerated Solvent Extractor and aqueous extracts (AE) were prepared under stirring. Their chemical fingerprint was evaluated by liquid chromatography–high resolution mass spectrometry (LC-HRMS). The pro- and anti-inflammatory effects, as well as the reduction in intracellular reactive oxygen and nitrogen species (ROS/RNS), of the different extracts were evaluated using non-stimulated and lipopolysaccharide-stimulated macrophages. Interestingly, AE were able to stimulate macrophages to produce pro-inflammatory cytokines (tumor necrosis factor -TNF-α, interleukin -IL-1β, and IL-6), and to generate ROS/RNS. Conversely, under an inflammatory scenario, all extracts reduced the amount of pro-inflammatory mediators. DE, alkylamides-enriched extracts, showed the strongest anti-inflammatory activity. Moreover, <i>E. purpurea</i> extracts demonstrated generally a more robust anti-inflammatory activity than clinically used anti-inflammatory drugs (dexamethasone, diclofenac, salicylic acid, and celecoxib). Therefore, <i>E. purpurea</i> extracts may be used to develop new effective therapeutic formulations for disorders in which the immune system is either overactive or impaired. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-06 2022-11-06T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/81148 |
url |
https://hdl.handle.net/1822/81148 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vieira, S.F.; Gonçalves, V.M.F.; Llaguno, C.P.; Macías, F.; Tiritan, M.E.; Reis, R.L.; Ferreira, H.; Neves, N.M. On the Bioactivity of Echinacea purpurea Extracts to Modulate the Production of Inflammatory Mediators. Int. J. Mol. Sci. 2022, 23, 13616. https://doi.org/10.3390/ijms232113616 1661-6596 1661-6596 10.3390/ijms232113616 36362404 https://www.mdpi.com/1422-0067/23/21/13616 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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