Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012

Detalhes bibliográficos
Autor(a) principal: Chasqueira, Maria de Jesus
Data de Publicação: 2014
Outros Autores: Rodrigues, Luís, Nascimento, M, Ramos, M, Marques,  Teresa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/23557
Resumo: This study was supported by the Portuguese Foundation for Science and Technology (FCT) [grant number PTDC/SAU-ESA/64269/2006].
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spelling Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012LEGIONNAIRES-DISEASENEUASEQUENCE-BASED SCHEMESEROGROUPSCULTURESTRAINSDISCRIMINATORY ABILITYMONOCLONAL-ANTIBODY SUBGROUPSMONOCLONAL-ANTIBODY SUBGROUPSSEQUENCE-BASED SCHEMEDISCRIMINATORY ABILITYLEGIONNAIRES-DISEASESEROGROUPSCULTURESTRAINSNEUAThis study was supported by the Portuguese Foundation for Science and Technology (FCT) [grant number PTDC/SAU-ESA/64269/2006].The genetic diversity of 89 clinical Legionella isolates, collected between 1987 and 2012, in 22 hospitals from the five regions of Portugal, was analysed in this study using monoclonal antibodies (MAbs) of the Dresden panel and the sequence-based typing (SBT) protocol. The eBURST algorithm was used to infer levels of relatedness between isolates. All isolates collected were Legionella pneumophila, which were further characterised into four subgroups by MAbs, and 30 sequence types (STs) by SBT. Twelve of the STs were unique to Portugal; one of them (ST100) was represented by 32 epidemiologically related isolates. The ST44 was the profile with the highest number of epidemiologically unrelated isolates. The eBURST analyses indicate that, within the group formed by the 30 STs identified in this study, 17 STs were genetically close to at least another ST in the group. The comparison between the eBURST diagrams obtained with the STs from this study and the entire SBT database of the European Working Group for Legionella, showed that 24 (seven of them unique to Portugal) of our 30 STs were related with STs identified in others countries. These results suggest that the population of L. pneumophila clinical strains in Portugal includes both worldwide and local strains.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNChasqueira, Maria de JesusRodrigues, LuísNascimento, MRamos, MMarques,  Teresa2017-09-25T22:00:19Z2014-11-202014-11-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://hdl.handle.net/10362/23557eng1560-7917PURE: 117152https://doi.org/10.2807/1560-7917.ES2014.19.46.20965info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:11:49Zoai:run.unl.pt:10362/23557Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:27:50.072859Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012
title Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012
spellingShingle Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012
Chasqueira, Maria de Jesus
LEGIONNAIRES-DISEASE
NEUA
SEQUENCE-BASED SCHEME
SEROGROUPS
CULTURE
STRAINS
DISCRIMINATORY ABILITY
MONOCLONAL-ANTIBODY SUBGROUPS
MONOCLONAL-ANTIBODY SUBGROUPS
SEQUENCE-BASED SCHEME
DISCRIMINATORY ABILITY
LEGIONNAIRES-DISEASE
SEROGROUPS
CULTURE
STRAINS
NEUA
title_short Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012
title_full Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012
title_fullStr Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012
title_full_unstemmed Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012
title_sort Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012
author Chasqueira, Maria de Jesus
author_facet Chasqueira, Maria de Jesus
Rodrigues, Luís
Nascimento, M
Ramos, M
Marques,  Teresa
author_role author
author2 Rodrigues, Luís
Nascimento, M
Ramos, M
Marques,  Teresa
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Chasqueira, Maria de Jesus
Rodrigues, Luís
Nascimento, M
Ramos, M
Marques,  Teresa
dc.subject.por.fl_str_mv LEGIONNAIRES-DISEASE
NEUA
SEQUENCE-BASED SCHEME
SEROGROUPS
CULTURE
STRAINS
DISCRIMINATORY ABILITY
MONOCLONAL-ANTIBODY SUBGROUPS
MONOCLONAL-ANTIBODY SUBGROUPS
SEQUENCE-BASED SCHEME
DISCRIMINATORY ABILITY
LEGIONNAIRES-DISEASE
SEROGROUPS
CULTURE
STRAINS
NEUA
topic LEGIONNAIRES-DISEASE
NEUA
SEQUENCE-BASED SCHEME
SEROGROUPS
CULTURE
STRAINS
DISCRIMINATORY ABILITY
MONOCLONAL-ANTIBODY SUBGROUPS
MONOCLONAL-ANTIBODY SUBGROUPS
SEQUENCE-BASED SCHEME
DISCRIMINATORY ABILITY
LEGIONNAIRES-DISEASE
SEROGROUPS
CULTURE
STRAINS
NEUA
description This study was supported by the Portuguese Foundation for Science and Technology (FCT) [grant number PTDC/SAU-ESA/64269/2006].
publishDate 2014
dc.date.none.fl_str_mv 2014-11-20
2014-11-20T00:00:00Z
2017-09-25T22:00:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/23557
url http://hdl.handle.net/10362/23557
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1560-7917
PURE: 117152
https://doi.org/10.2807/1560-7917.ES2014.19.46.20965
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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