Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/23557 |
Resumo: | This study was supported by the Portuguese Foundation for Science and Technology (FCT) [grant number PTDC/SAU-ESA/64269/2006]. |
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Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012LEGIONNAIRES-DISEASENEUASEQUENCE-BASED SCHEMESEROGROUPSCULTURESTRAINSDISCRIMINATORY ABILITYMONOCLONAL-ANTIBODY SUBGROUPSMONOCLONAL-ANTIBODY SUBGROUPSSEQUENCE-BASED SCHEMEDISCRIMINATORY ABILITYLEGIONNAIRES-DISEASESEROGROUPSCULTURESTRAINSNEUAThis study was supported by the Portuguese Foundation for Science and Technology (FCT) [grant number PTDC/SAU-ESA/64269/2006].The genetic diversity of 89 clinical Legionella isolates, collected between 1987 and 2012, in 22 hospitals from the five regions of Portugal, was analysed in this study using monoclonal antibodies (MAbs) of the Dresden panel and the sequence-based typing (SBT) protocol. The eBURST algorithm was used to infer levels of relatedness between isolates. All isolates collected were Legionella pneumophila, which were further characterised into four subgroups by MAbs, and 30 sequence types (STs) by SBT. Twelve of the STs were unique to Portugal; one of them (ST100) was represented by 32 epidemiologically related isolates. The ST44 was the profile with the highest number of epidemiologically unrelated isolates. The eBURST analyses indicate that, within the group formed by the 30 STs identified in this study, 17 STs were genetically close to at least another ST in the group. The comparison between the eBURST diagrams obtained with the STs from this study and the entire SBT database of the European Working Group for Legionella, showed that 24 (seven of them unique to Portugal) of our 30 STs were related with STs identified in others countries. These results suggest that the population of L. pneumophila clinical strains in Portugal includes both worldwide and local strains.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNChasqueira, Maria de JesusRodrigues, LuísNascimento, MRamos, MMarques, Teresa2017-09-25T22:00:19Z2014-11-202014-11-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://hdl.handle.net/10362/23557eng1560-7917PURE: 117152https://doi.org/10.2807/1560-7917.ES2014.19.46.20965info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:11:49Zoai:run.unl.pt:10362/23557Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:27:50.072859Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012 |
title |
Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012 |
spellingShingle |
Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012 Chasqueira, Maria de Jesus LEGIONNAIRES-DISEASE NEUA SEQUENCE-BASED SCHEME SEROGROUPS CULTURE STRAINS DISCRIMINATORY ABILITY MONOCLONAL-ANTIBODY SUBGROUPS MONOCLONAL-ANTIBODY SUBGROUPS SEQUENCE-BASED SCHEME DISCRIMINATORY ABILITY LEGIONNAIRES-DISEASE SEROGROUPS CULTURE STRAINS NEUA |
title_short |
Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012 |
title_full |
Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012 |
title_fullStr |
Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012 |
title_full_unstemmed |
Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012 |
title_sort |
Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012 |
author |
Chasqueira, Maria de Jesus |
author_facet |
Chasqueira, Maria de Jesus Rodrigues, Luís Nascimento, M Ramos, M Marques, Teresa |
author_role |
author |
author2 |
Rodrigues, Luís Nascimento, M Ramos, M Marques, Teresa |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
dc.contributor.author.fl_str_mv |
Chasqueira, Maria de Jesus Rodrigues, Luís Nascimento, M Ramos, M Marques, Teresa |
dc.subject.por.fl_str_mv |
LEGIONNAIRES-DISEASE NEUA SEQUENCE-BASED SCHEME SEROGROUPS CULTURE STRAINS DISCRIMINATORY ABILITY MONOCLONAL-ANTIBODY SUBGROUPS MONOCLONAL-ANTIBODY SUBGROUPS SEQUENCE-BASED SCHEME DISCRIMINATORY ABILITY LEGIONNAIRES-DISEASE SEROGROUPS CULTURE STRAINS NEUA |
topic |
LEGIONNAIRES-DISEASE NEUA SEQUENCE-BASED SCHEME SEROGROUPS CULTURE STRAINS DISCRIMINATORY ABILITY MONOCLONAL-ANTIBODY SUBGROUPS MONOCLONAL-ANTIBODY SUBGROUPS SEQUENCE-BASED SCHEME DISCRIMINATORY ABILITY LEGIONNAIRES-DISEASE SEROGROUPS CULTURE STRAINS NEUA |
description |
This study was supported by the Portuguese Foundation for Science and Technology (FCT) [grant number PTDC/SAU-ESA/64269/2006]. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-11-20 2014-11-20T00:00:00Z 2017-09-25T22:00:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/23557 |
url |
http://hdl.handle.net/10362/23557 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1560-7917 PURE: 117152 https://doi.org/10.2807/1560-7917.ES2014.19.46.20965 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137905138991104 |