Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts

Detalhes bibliográficos
Autor(a) principal: Pavlić, Branimir
Data de Publicação: 2022
Outros Autores: Mrkonjić, Živan, Teslić, Nemanja, Kljakić, Aleksandra Cvetanović, Pojić, Milica, Mandić, Anamarija, Stupar, Alena, Santos, Filipa, Duarte, Ana Rita C., Mišan, Aleksandra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/143805
Resumo: Funding Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT Funding: This research was supported by the Science Fund of the Republic of Serbia, 6060592, Natural Deep Eutectic Solvents for Green Agri-Food Solutions—DEStiny and 7750168, Novel extracts and bioactive compounds from under-utilized resources for high-value applications—BioUtilize. This project received funding from the European Union’s Horizon 2020—European Research Council (ERC)—under grant agreement No ERC-2016-CoG 725034. T Funding Information: This research was supported by the Science Fund of the Republic of Serbia, 6060592, Natural Deep Eutectic Solvents for Green Agri-Food Solutions?DEStiny and 7750168, Novel extracts and bioactive compounds from under-utilized resources for high-value applications?BioUtilize. This project received funding from the European Union?s Horizon 2020?European Research Council (ERC)?under grant agreement No ERC-2016-CoG 725034. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
id RCAP_7749be3ddd1d4d0110037b6cceb24fb7
oai_identifier_str oai:run.unl.pt:10362/143805
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) ExtractsAntioxidant activityNADESPolyphenolsRSM optimizationThymus serpyllum LAnalytical ChemistryChemistry (miscellaneous)Molecular MedicinePharmaceutical ScienceDrug DiscoveryPhysical and Theoretical ChemistryOrganic ChemistryFunding Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT Funding: This research was supported by the Science Fund of the Republic of Serbia, 6060592, Natural Deep Eutectic Solvents for Green Agri-Food Solutions—DEStiny and 7750168, Novel extracts and bioactive compounds from under-utilized resources for high-value applications—BioUtilize. This project received funding from the European Union’s Horizon 2020—European Research Council (ERC)—under grant agreement No ERC-2016-CoG 725034. T Funding Information: This research was supported by the Science Fund of the Republic of Serbia, 6060592, Natural Deep Eutectic Solvents for Green Agri-Food Solutions?DEStiny and 7750168, Novel extracts and bioactive compounds from under-utilized resources for high-value applications?BioUtilize. This project received funding from the European Union?s Horizon 2020?European Research Council (ERC)?under grant agreement No ERC-2016-CoG 725034. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Wild thyme (Thymus serpyllum L.) herbal dust has been recognized as a potential underutilized resource for the recovery of antioxidants. The aim of this paper was to optimize natural deep eutectic solvent (NADES) extraction of polyphenols to obtain improved antioxidant activity of extracts determined by selected in vitro assays (DPPH, FRAP, and ABTS). Twenty different NADES systems were investigated in the first step of the screening of the extraction solvent and L-proline (Pro)–glycerine (Gly) based solvents provided the best results. Preliminary experiments organized by 25−1 fractional factorial design narrowed down the number of extraction factors from five (temperature, extraction time, NADES type, water content and L/S ratio) to three and determined their experimental domain for the final step. A face-centered central composite design with temperature (40–55–70 °C), extraction time (60–120–180 min) and L/S ratio (10–20–30 g NADES/g sample) was applied for influence analysis and process optimization. Multi-response optimization suggested a temperature of 65 °C, time of extraction of 180 min and L/S ratio of 28 g NADES/g DW as optimal extraction parameters. Experimental validation confirmed good agreement between experimental and predicted results in the extract obtained at optimal conditions and the interactions in the most suitable NADES (N16; Pro–Gly–H2O; 1:2:1) were confirmed by the1H-NMR.DQ - Departamento de QuímicaLAQV@REQUIMTERUNPavlić, BranimirMrkonjić, ŽivanTeslić, NemanjaKljakić, Aleksandra CvetanovićPojić, MilicaMandić, AnamarijaStupar, AlenaSantos, FilipaDuarte, Ana Rita C.Mišan, Aleksandra2022-09-16T22:28:56Z2022-022022-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20application/pdfhttp://hdl.handle.net/10362/143805eng1420-3049PURE: 46583301https://doi.org/10.3390/molecules27051508info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:22:35Zoai:run.unl.pt:10362/143805Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:09.343809Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts
title Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts
spellingShingle Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts
Pavlić, Branimir
Antioxidant activity
NADES
Polyphenols
RSM optimization
Thymus serpyllum L
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
title_short Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts
title_full Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts
title_fullStr Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts
title_full_unstemmed Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts
title_sort Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts
author Pavlić, Branimir
author_facet Pavlić, Branimir
Mrkonjić, Živan
Teslić, Nemanja
Kljakić, Aleksandra Cvetanović
Pojić, Milica
Mandić, Anamarija
Stupar, Alena
Santos, Filipa
Duarte, Ana Rita C.
Mišan, Aleksandra
author_role author
author2 Mrkonjić, Živan
Teslić, Nemanja
Kljakić, Aleksandra Cvetanović
Pojić, Milica
Mandić, Anamarija
Stupar, Alena
Santos, Filipa
Duarte, Ana Rita C.
Mišan, Aleksandra
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
LAQV@REQUIMTE
RUN
dc.contributor.author.fl_str_mv Pavlić, Branimir
Mrkonjić, Živan
Teslić, Nemanja
Kljakić, Aleksandra Cvetanović
Pojić, Milica
Mandić, Anamarija
Stupar, Alena
Santos, Filipa
Duarte, Ana Rita C.
Mišan, Aleksandra
dc.subject.por.fl_str_mv Antioxidant activity
NADES
Polyphenols
RSM optimization
Thymus serpyllum L
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
topic Antioxidant activity
NADES
Polyphenols
RSM optimization
Thymus serpyllum L
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
description Funding Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT Funding: This research was supported by the Science Fund of the Republic of Serbia, 6060592, Natural Deep Eutectic Solvents for Green Agri-Food Solutions—DEStiny and 7750168, Novel extracts and bioactive compounds from under-utilized resources for high-value applications—BioUtilize. This project received funding from the European Union’s Horizon 2020—European Research Council (ERC)—under grant agreement No ERC-2016-CoG 725034. T Funding Information: This research was supported by the Science Fund of the Republic of Serbia, 6060592, Natural Deep Eutectic Solvents for Green Agri-Food Solutions?DEStiny and 7750168, Novel extracts and bioactive compounds from under-utilized resources for high-value applications?BioUtilize. This project received funding from the European Union?s Horizon 2020?European Research Council (ERC)?under grant agreement No ERC-2016-CoG 725034. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-16T22:28:56Z
2022-02
2022-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/143805
url http://hdl.handle.net/10362/143805
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1420-3049
PURE: 46583301
https://doi.org/10.3390/molecules27051508
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138106263207936

Registros relacionados