A chemotaxis operon in the bacterium Desulfovibrio gigas is induced under several growth conditions

Detalhes bibliográficos
Autor(a) principal: Félix, Rute
Data de Publicação: 2006
Outros Autores: Rodrigues, Rute, Machado, Patrícia, Oliveira, Solange, Rodrigues-Pousada, Claudina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/1358
Resumo: Abstract: The chemosensory system of bacteria controls their motility and behaviour in different environments. In the present study, we report the identification of the first chemotaxis operon in Desulfovibrio gigas. Amino acid sequence analysis revealed seven coding regions for polypeptides with a high similarity to chemotaxis proteins from other organisms. D. gigas chemotaxis operon has a similar genetic organisation to chemotaxis operons found in the sequenced genomes of Desulfovibrio desulfuricans and Desulfovibrio vulgaris. Control of gene expression was assessed by real-time reverse transcription-PCR in cells grown under different conditions. mRNA levels were enhanced in the presence of thiosulfate and sulfite and decreased upon exposure to NO. No effect was observed in the presence of O-2, NaNO2, pyruvate or fumarate. These results show that the expression of the chemotaxis operon is enhanced in the presence of thiosulfate and sulfite indicating that under these compounds a chemotactic response seems to be triggered in D. gigas.
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spelling A chemotaxis operon in the bacterium Desulfovibrio gigas is induced under several growth conditionsDesulfovibrio gigaschemotaxis operonAbstract: The chemosensory system of bacteria controls their motility and behaviour in different environments. In the present study, we report the identification of the first chemotaxis operon in Desulfovibrio gigas. Amino acid sequence analysis revealed seven coding regions for polypeptides with a high similarity to chemotaxis proteins from other organisms. D. gigas chemotaxis operon has a similar genetic organisation to chemotaxis operons found in the sequenced genomes of Desulfovibrio desulfuricans and Desulfovibrio vulgaris. Control of gene expression was assessed by real-time reverse transcription-PCR in cells grown under different conditions. mRNA levels were enhanced in the presence of thiosulfate and sulfite and decreased upon exposure to NO. No effect was observed in the presence of O-2, NaNO2, pyruvate or fumarate. These results show that the expression of the chemotaxis operon is enhanced in the presence of thiosulfate and sulfite indicating that under these compounds a chemotactic response seems to be triggered in D. gigas.2008-12-03T16:13:42Z2008-12-032006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article37716 bytesapplication/pdfhttp://hdl.handle.net/10174/1358http://hdl.handle.net/10174/1358engpag 56-64DNA SEQUENCE117livrendndndndnd226Félix, RuteRodrigues, RuteMachado, PatríciaOliveira, SolangeRodrigues-Pousada, Claudinainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:37:17Zoai:dspace.uevora.pt:10174/1358Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:57:27.188430Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A chemotaxis operon in the bacterium Desulfovibrio gigas is induced under several growth conditions
title A chemotaxis operon in the bacterium Desulfovibrio gigas is induced under several growth conditions
spellingShingle A chemotaxis operon in the bacterium Desulfovibrio gigas is induced under several growth conditions
Félix, Rute
Desulfovibrio gigas
chemotaxis operon
title_short A chemotaxis operon in the bacterium Desulfovibrio gigas is induced under several growth conditions
title_full A chemotaxis operon in the bacterium Desulfovibrio gigas is induced under several growth conditions
title_fullStr A chemotaxis operon in the bacterium Desulfovibrio gigas is induced under several growth conditions
title_full_unstemmed A chemotaxis operon in the bacterium Desulfovibrio gigas is induced under several growth conditions
title_sort A chemotaxis operon in the bacterium Desulfovibrio gigas is induced under several growth conditions
author Félix, Rute
author_facet Félix, Rute
Rodrigues, Rute
Machado, Patrícia
Oliveira, Solange
Rodrigues-Pousada, Claudina
author_role author
author2 Rodrigues, Rute
Machado, Patrícia
Oliveira, Solange
Rodrigues-Pousada, Claudina
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Félix, Rute
Rodrigues, Rute
Machado, Patrícia
Oliveira, Solange
Rodrigues-Pousada, Claudina
dc.subject.por.fl_str_mv Desulfovibrio gigas
chemotaxis operon
topic Desulfovibrio gigas
chemotaxis operon
description Abstract: The chemosensory system of bacteria controls their motility and behaviour in different environments. In the present study, we report the identification of the first chemotaxis operon in Desulfovibrio gigas. Amino acid sequence analysis revealed seven coding regions for polypeptides with a high similarity to chemotaxis proteins from other organisms. D. gigas chemotaxis operon has a similar genetic organisation to chemotaxis operons found in the sequenced genomes of Desulfovibrio desulfuricans and Desulfovibrio vulgaris. Control of gene expression was assessed by real-time reverse transcription-PCR in cells grown under different conditions. mRNA levels were enhanced in the presence of thiosulfate and sulfite and decreased upon exposure to NO. No effect was observed in the presence of O-2, NaNO2, pyruvate or fumarate. These results show that the expression of the chemotaxis operon is enhanced in the presence of thiosulfate and sulfite indicating that under these compounds a chemotactic response seems to be triggered in D. gigas.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01T00:00:00Z
2008-12-03T16:13:42Z
2008-12-03
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/1358
http://hdl.handle.net/10174/1358
url http://hdl.handle.net/10174/1358
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv pag 56-64
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