A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Rute
Data de Publicação: 2003
Outros Autores: Valente, Filipa, Pereira, Inês, Oliveira, Solange, Rodrigues-Pousada, Claudina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/2104
Resumo: Abstract: In the present study, we report the identification of an operon with six coding regions for a multisubunit membrane-bound [NiFe] hydrogenase in the genome of Desulfovibrio gigas. Sequence analysis of the deduced polypeptides reveals a high similarity to subunits of proteins belonging to the family of Ech hydrogenases. The operon is organised similarly to the operon coding for the Ech hydrogenase from Methanosarcina barkeri, suggesting that both encode very similar hydrogenases. Expression of the operon was detected by Northern blot and RT-PCR analyses, and the presence of the encoded proteins was examined by Western blotting. The possible role of this hydrogenase is discussed, relating it with a potential function in the H-2 cycling as a mechanism for energy conservation in D. gigas. The present study provides therefore valuable insights into the open question of the energy conserving mechanism in D. gigas
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spelling A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigashydrogenaseDesulfovibrio gigasAbstract: In the present study, we report the identification of an operon with six coding regions for a multisubunit membrane-bound [NiFe] hydrogenase in the genome of Desulfovibrio gigas. Sequence analysis of the deduced polypeptides reveals a high similarity to subunits of proteins belonging to the family of Ech hydrogenases. The operon is organised similarly to the operon coding for the Ech hydrogenase from Methanosarcina barkeri, suggesting that both encode very similar hydrogenases. Expression of the operon was detected by Northern blot and RT-PCR analyses, and the presence of the encoded proteins was examined by Western blotting. The possible role of this hydrogenase is discussed, relating it with a potential function in the H-2 cycling as a mechanism for energy conservation in D. gigas. The present study provides therefore valuable insights into the open question of the energy conserving mechanism in D. gigas2010-09-27T14:44:47Z2010-09-272003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article123736 bytesapplication/pdfhttp://hdl.handle.net/10174/2104http://hdl.handle.net/10174/2104engpag 366-375306BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONSlivrendndndndnd548Rodrigues, RuteValente, FilipaPereira, InêsOliveira, SolangeRodrigues-Pousada, Claudinainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:38:18Zoai:dspace.uevora.pt:10174/2104Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:57:53.236489Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas
title A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas
spellingShingle A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas
Rodrigues, Rute
hydrogenase
Desulfovibrio gigas
title_short A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas
title_full A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas
title_fullStr A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas
title_full_unstemmed A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas
title_sort A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas
author Rodrigues, Rute
author_facet Rodrigues, Rute
Valente, Filipa
Pereira, Inês
Oliveira, Solange
Rodrigues-Pousada, Claudina
author_role author
author2 Valente, Filipa
Pereira, Inês
Oliveira, Solange
Rodrigues-Pousada, Claudina
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues, Rute
Valente, Filipa
Pereira, Inês
Oliveira, Solange
Rodrigues-Pousada, Claudina
dc.subject.por.fl_str_mv hydrogenase
Desulfovibrio gigas
topic hydrogenase
Desulfovibrio gigas
description Abstract: In the present study, we report the identification of an operon with six coding regions for a multisubunit membrane-bound [NiFe] hydrogenase in the genome of Desulfovibrio gigas. Sequence analysis of the deduced polypeptides reveals a high similarity to subunits of proteins belonging to the family of Ech hydrogenases. The operon is organised similarly to the operon coding for the Ech hydrogenase from Methanosarcina barkeri, suggesting that both encode very similar hydrogenases. Expression of the operon was detected by Northern blot and RT-PCR analyses, and the presence of the encoded proteins was examined by Western blotting. The possible role of this hydrogenase is discussed, relating it with a potential function in the H-2 cycling as a mechanism for energy conservation in D. gigas. The present study provides therefore valuable insights into the open question of the energy conserving mechanism in D. gigas
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01T00:00:00Z
2010-09-27T14:44:47Z
2010-09-27
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/2104
http://hdl.handle.net/10174/2104
url http://hdl.handle.net/10174/2104
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv pag 366-375
306
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
livre
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nd
nd
nd
nd
548
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