Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's disease

Detalhes bibliográficos
Autor(a) principal: Savchenko, Ekaterina
Data de Publicação: 2018
Outros Autores: Marote, Ana Maria Franco Aveiro, Russ, Kaspar, Collin, Anna, Goldwurm, Stefano, Roybon, Laurent, Pomeshchik, Yuriy
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/59944
Resumo: Skin fibroblasts were collected from a 44-year-old patient with sporadic case of Parkinson's disease (PD). The non-integrating Sendai virus vector encoding OCT3/4, SOX2, c-MYC and KLF4 was used to reprogram fibroblasts into induced pluripotent stem cells (iPSCs). Generated iPSCs had normal karyotypes, expressed common stem cell markers, and were capable of differentiating into all three germ layers. Generated line could be used for PD modeling to understand the mechanisms that influence the disorder.
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spelling Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's diseaseAdultCell DifferentiationCells, CulturedFemaleFibroblastsGerm LayersHumansInduced Pluripotent Stem CellsKaryotypeParkinson DiseaseScience & TechnologySkin fibroblasts were collected from a 44-year-old patient with sporadic case of Parkinson's disease (PD). The non-integrating Sendai virus vector encoding OCT3/4, SOX2, c-MYC and KLF4 was used to reprogram fibroblasts into induced pluripotent stem cells (iPSCs). Generated iPSCs had normal karyotypes, expressed common stem cell markers, and were capable of differentiating into all three germ layers. Generated line could be used for PD modeling to understand the mechanisms that influence the disorder.We thank AnnaKarin Oldén and Marianne Juhlin, for their technical support. We are also thankful to the ‘Cell Line and DNA Biobank from Patients affected by Genetic Diseases’ (Istituto G. Gaslini, Genova, Italy) and the ‘Parkinson Institute Biobank, members of the Telethon Network of Genetic Biobanks (http://biobanknetwork.telethon.it; project no. GTB12001) funded by Telethon Italy, for providing fibroblasts samples. This work was supported by the Strategic Research Environment MultiPark at Lund University, the strong research environment BAGADILICO (349-2007-8626), the Swedish Parkinson Foundation (Parkinsonfonden; grant 899/16), the Swedish Research Council (grant 2015-03684 to LR), Finnish Cultural Foundation (grant 00161167 to YP) and the Portuguese Foundation for Science and Technology for the doctoral fellowship - PDE/BDE/113598/2015 to AM.Elsevier Science BVUniversidade do MinhoSavchenko, EkaterinaMarote, Ana Maria Franco AveiroRuss, KasparCollin, AnnaGoldwurm, StefanoRoybon, LaurentPomeshchik, Yuriy20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/59944eng1873-506110.1016/j.scr.2018.01.00229334629info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:56:17Zoai:repositorium.sdum.uminho.pt:1822/59944Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:45:55.173264Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's disease
title Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's disease
spellingShingle Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's disease
Savchenko, Ekaterina
Adult
Cell Differentiation
Cells, Cultured
Female
Fibroblasts
Germ Layers
Humans
Induced Pluripotent Stem Cells
Karyotype
Parkinson Disease
Science & Technology
title_short Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's disease
title_full Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's disease
title_fullStr Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's disease
title_full_unstemmed Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's disease
title_sort Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's disease
author Savchenko, Ekaterina
author_facet Savchenko, Ekaterina
Marote, Ana Maria Franco Aveiro
Russ, Kaspar
Collin, Anna
Goldwurm, Stefano
Roybon, Laurent
Pomeshchik, Yuriy
author_role author
author2 Marote, Ana Maria Franco Aveiro
Russ, Kaspar
Collin, Anna
Goldwurm, Stefano
Roybon, Laurent
Pomeshchik, Yuriy
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Savchenko, Ekaterina
Marote, Ana Maria Franco Aveiro
Russ, Kaspar
Collin, Anna
Goldwurm, Stefano
Roybon, Laurent
Pomeshchik, Yuriy
dc.subject.por.fl_str_mv Adult
Cell Differentiation
Cells, Cultured
Female
Fibroblasts
Germ Layers
Humans
Induced Pluripotent Stem Cells
Karyotype
Parkinson Disease
Science & Technology
topic Adult
Cell Differentiation
Cells, Cultured
Female
Fibroblasts
Germ Layers
Humans
Induced Pluripotent Stem Cells
Karyotype
Parkinson Disease
Science & Technology
description Skin fibroblasts were collected from a 44-year-old patient with sporadic case of Parkinson's disease (PD). The non-integrating Sendai virus vector encoding OCT3/4, SOX2, c-MYC and KLF4 was used to reprogram fibroblasts into induced pluripotent stem cells (iPSCs). Generated iPSCs had normal karyotypes, expressed common stem cell markers, and were capable of differentiating into all three germ layers. Generated line could be used for PD modeling to understand the mechanisms that influence the disorder.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/59944
url http://hdl.handle.net/1822/59944
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1873-5061
10.1016/j.scr.2018.01.002
29334629
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Science BV
publisher.none.fl_str_mv Elsevier Science BV
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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