Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells

Detalhes bibliográficos
Autor(a) principal: Antunes, Joana
Data de Publicação: 2023
Outros Autores: Sobral, Paula, Martins, Marta, Branco, Vasco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/163986
Resumo: Funding Information: This research was supported by Fundação para a Ciência e Tecnologia , I.P, through: Norma Transitória - DL57/2016/CP1376/CT002 , VB; through the Scientific Employment Stimulus - Institutional Call ( CEECINST/00102/2018 ), MM. ULisboa’s Strategic Project ( UID/DTP/04138/2020 ). Publisher Copyright: © 2023 The Authors
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spelling Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cellsInflammationIntestineMicrogliaNanoplasticsp38TLR4ToxicologyPharmacologyHealth, Toxicology and MutagenesisSDG 3 - Good Health and Well-beingFunding Information: This research was supported by Fundação para a Ciência e Tecnologia , I.P, through: Norma Transitória - DL57/2016/CP1376/CT002 , VB; through the Scientific Employment Stimulus - Institutional Call ( CEECINST/00102/2018 ), MM. ULisboa’s Strategic Project ( UID/DTP/04138/2020 ). Publisher Copyright: © 2023 The AuthorsThe crescent presence of nanoplastics in the environment raises concerns regarding their potential impact on health. This study exposed human colon adenocarcinoma cells (HT29) and microglia cells (N9) to nanoplastics (25 nm, 50 nm, and 100 nm Polystyrene) to investigate their inflammatory responses, which are vital for body's defence. Although cytotoxicity remained generally low, HT29 cells exhibited a notable upregulation of p50 and p38 expression, concomitant with elevated TLR4 expression, in contrast with N9 cells that showed a less pronounced upregulation of these proteins. Additionally, nanoplastic exposure increased IL-1ß levels, partially attenuated by pre-exposure to TLR4 or p38 inhibitors. Intriguingly, N9 cells exposed to nanoplastics exhibited substantial increases in iNOS mRNA. This effect was entirely prevented by pre-exposure to TLR4 or p38 inhibitors, while TNF-α mRNA levels remained relatively stable. These findings underscore the potential of nanoplastics to activate inflammatory pathways, with response kinetics varying depending on the cell type.MARE - Centro de Ciências do Mar e do AmbienteDCEA - Departamento de Ciências e Engenharia do AmbienteRUNAntunes, JoanaSobral, PaulaMartins, MartaBranco, Vasco2024-02-22T23:53:26Z2023-112023-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/163986eng1382-6689PURE: 83892213https://doi.org/10.1016/j.etap.2023.104298info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:50:04Zoai:run.unl.pt:10362/163986Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:59:58.703620Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
title Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
spellingShingle Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
Antunes, Joana
Inflammation
Intestine
Microglia
Nanoplastics
p38
TLR4
Toxicology
Pharmacology
Health, Toxicology and Mutagenesis
SDG 3 - Good Health and Well-being
title_short Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
title_full Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
title_fullStr Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
title_full_unstemmed Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
title_sort Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
author Antunes, Joana
author_facet Antunes, Joana
Sobral, Paula
Martins, Marta
Branco, Vasco
author_role author
author2 Sobral, Paula
Martins, Marta
Branco, Vasco
author2_role author
author
author
dc.contributor.none.fl_str_mv MARE - Centro de Ciências do Mar e do Ambiente
DCEA - Departamento de Ciências e Engenharia do Ambiente
RUN
dc.contributor.author.fl_str_mv Antunes, Joana
Sobral, Paula
Martins, Marta
Branco, Vasco
dc.subject.por.fl_str_mv Inflammation
Intestine
Microglia
Nanoplastics
p38
TLR4
Toxicology
Pharmacology
Health, Toxicology and Mutagenesis
SDG 3 - Good Health and Well-being
topic Inflammation
Intestine
Microglia
Nanoplastics
p38
TLR4
Toxicology
Pharmacology
Health, Toxicology and Mutagenesis
SDG 3 - Good Health and Well-being
description Funding Information: This research was supported by Fundação para a Ciência e Tecnologia , I.P, through: Norma Transitória - DL57/2016/CP1376/CT002 , VB; through the Scientific Employment Stimulus - Institutional Call ( CEECINST/00102/2018 ), MM. ULisboa’s Strategic Project ( UID/DTP/04138/2020 ). Publisher Copyright: © 2023 The Authors
publishDate 2023
dc.date.none.fl_str_mv 2023-11
2023-11-01T00:00:00Z
2024-02-22T23:53:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/163986
url http://hdl.handle.net/10362/163986
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1382-6689
PURE: 83892213
https://doi.org/10.1016/j.etap.2023.104298
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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