Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells

Detalhes bibliográficos
Autor(a) principal: Antunes, Joana
Data de Publicação: 2023
Outros Autores: Sobral, Paula, Martins, Marta, Branco, Vasco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/61917
Resumo: © 2023 The Authors. Published by Elsevier B.V.
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spelling Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cellsNanoplasticsIntestineInflammationTLR4Microgliap38© 2023 The Authors. Published by Elsevier B.V.The crescent presence of nanoplastics in the environment raises concerns regarding their potential impact on health. This study exposed human colon adenocarcinoma cells (HT29) and microglia cells (N9) to nanoplastics (25 nm, 50 nm, and 100 nm Polystyrene) to investigate their inflammatory responses, which are vital for body's defence. Although cytotoxicity remained generally low, HT29 cells exhibited a notable upregulation of p50 and p38 expression, concomitant with elevated TLR4 expression, in contrast with N9 cells that showed a less pronounced upregulation of these proteins. Additionally, nanoplastic exposure increased IL-1ß levels, partially attenuated by pre-exposure to TLR4 or p38 inhibitors. Intriguingly, N9 cells exposed to nanoplastics exhibited substantial increases in iNOS mRNA. This effect was entirely prevented by pre-exposure to TLR4 or p38 inhibitors, while TNF-α mRNA levels remained relatively stable. These findings underscore the potential of nanoplastics to activate inflammatory pathways, with response kinetics varying depending on the cell type.This research was supported by Fundação para a Ciência e Tecnologia, I.P, through: the Ph.D grant (SFRH/BD/144333/2019), JA; Norma Transitória - DL57/2016/CP1376/CT002, VB; through the Scientific Employment Stimulus - Institutional Call (CEECINST/00102/2018), MM; Nanoplastox Project – Nanoplastic Toxicity: from gut inflammation to systemic effects (2022.04884. PTDC); PAHMIX project - Mixtures of Environmental Carcinogens: a molecular approach to improve environmental risk assessment strategies (PTDC/CTA-AMB/29173/2017); MARE - Marine and Environmental Sciences Centre Strategic Project (UIDB/04292/2020), Associate Laboratory ARNET (LA/P/0069/2020) and iMed. ULisboa’s Strategic Project (UID/DTP/04138/2020). The authors acknowledge Isabella Bramatti, Beatriz Matos and Neusa Figueiredo from the Marine and Environmental Sciences Centre and Carolina Carola Alfenim from University of Lisbon for their important assistance. Thanks is due to Katherine Mahoney, for revising the text. The authors acknowledge iMed.ULisboa, Faculty of Pharmacy, University of Lisbon for providing the cells tested in the present study.ElsevierRepositório da Universidade de LisboaAntunes, JoanaSobral, PaulaMartins, MartaBranco, Vasco2024-01-18T15:56:34Z2023-102023-10-21T21:51:33Z2023-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/61917engAntunes J, Sobral P, Martins M, Branco V. Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells. Environmental Toxicology and Pharmacology [Internet]. novembro de 2023;104:104298. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S1382668923002405cv-prod-337125510.1016/j.etap.2023.104298info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-22T01:20:26Zoai:repositorio.ul.pt:10451/61917Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:56:16.493380Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
title Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
spellingShingle Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
Antunes, Joana
Nanoplastics
Intestine
Inflammation
TLR4
Microglia
p38
title_short Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
title_full Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
title_fullStr Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
title_full_unstemmed Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
title_sort Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells
author Antunes, Joana
author_facet Antunes, Joana
Sobral, Paula
Martins, Marta
Branco, Vasco
author_role author
author2 Sobral, Paula
Martins, Marta
Branco, Vasco
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Antunes, Joana
Sobral, Paula
Martins, Marta
Branco, Vasco
dc.subject.por.fl_str_mv Nanoplastics
Intestine
Inflammation
TLR4
Microglia
p38
topic Nanoplastics
Intestine
Inflammation
TLR4
Microglia
p38
description © 2023 The Authors. Published by Elsevier B.V.
publishDate 2023
dc.date.none.fl_str_mv 2023-10
2023-10-21T21:51:33Z
2023-10-01T00:00:00Z
2024-01-18T15:56:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/61917
url http://hdl.handle.net/10451/61917
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Antunes J, Sobral P, Martins M, Branco V. Nanoplastics activate a TLR4/p38-mediated pro-inflammatory response in human intestinal and mouse microglia cells. Environmental Toxicology and Pharmacology [Internet]. novembro de 2023;104:104298. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S1382668923002405
cv-prod-3371255
10.1016/j.etap.2023.104298
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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