Macroalgae from S. Miguel Island as a potential source of antiproliferative and antioxidant products
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.3/1556 |
Resumo: | Ulva compressa, Gelidium microdon, Osmundea pinnatifida, Fucus spiralis and Cystoseira abies-marina from the coast of S. Miguel Island were screened for their in vitro cytotoxicity against HeLa tumour cell line, antioxidant potential and total phenolic content. From each alga, the hexane fraction (HF) of the methanol extract, the methanol residue (MF) and the dichloromethane extract (DE), were obtained and evaluated. The highest antiproliferative activity against HeLa cell line was found in C. abies-marina and F. spiralis DE (IC50 8.8 μg/mL and 10.7 μg/mL, respectively), and presented selective cytotoxicity, when tested against Vero cell line. Fluorescence microscopy studies of the two most active extracts suggest an apoptosis-inducing activity. Concerning the antioxidant activity, high values were found on F. spiralis MF and HF (EC50 0.62 μg/mL and 2.01 μg/mL, respectively), even higher than those obtained for trolox and quercetin. This high antioxidant activity in F. spiralis MF can be explained by its content in phenolic compounds, but not for HF, where the antioxidant compounds must be less polar. These results suggest that some macroalgae from the Azorean sea have great potential which could be considered for future applications in medicine, food production or cosmetics industry. |
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Macroalgae from S. Miguel Island as a potential source of antiproliferative and antioxidant productsAzores Marine MacroalgaeCytotoxicityHeLaRadical Scavenging ActivityTotal Phenolic ContentUlva compressa, Gelidium microdon, Osmundea pinnatifida, Fucus spiralis and Cystoseira abies-marina from the coast of S. Miguel Island were screened for their in vitro cytotoxicity against HeLa tumour cell line, antioxidant potential and total phenolic content. From each alga, the hexane fraction (HF) of the methanol extract, the methanol residue (MF) and the dichloromethane extract (DE), were obtained and evaluated. The highest antiproliferative activity against HeLa cell line was found in C. abies-marina and F. spiralis DE (IC50 8.8 μg/mL and 10.7 μg/mL, respectively), and presented selective cytotoxicity, when tested against Vero cell line. Fluorescence microscopy studies of the two most active extracts suggest an apoptosis-inducing activity. Concerning the antioxidant activity, high values were found on F. spiralis MF and HF (EC50 0.62 μg/mL and 2.01 μg/mL, respectively), even higher than those obtained for trolox and quercetin. This high antioxidant activity in F. spiralis MF can be explained by its content in phenolic compounds, but not for HF, where the antioxidant compounds must be less polar. These results suggest that some macroalgae from the Azorean sea have great potential which could be considered for future applications in medicine, food production or cosmetics industry.Universidade dos AçoresRepositório da Universidade dos AçoresBarreto, Maria do CarmoMendonça, Emanuel A.Gouveia, Vera F.Anjos, CarolinaMedeiros, Joana S.Seca, Ana M. L.Neto, Ana I.2013-01-09T13:01:25Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.3/1556eng"ARQUIPÉLAGO. Life and Marine Sciences". ISSN 0873-4704. Nº 29 (2012): 53-580873-4704info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-20T14:29:19Zoai:repositorio.uac.pt:10400.3/1556Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:24:26.318747Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Macroalgae from S. Miguel Island as a potential source of antiproliferative and antioxidant products |
title |
Macroalgae from S. Miguel Island as a potential source of antiproliferative and antioxidant products |
spellingShingle |
Macroalgae from S. Miguel Island as a potential source of antiproliferative and antioxidant products Barreto, Maria do Carmo Azores Marine Macroalgae Cytotoxicity HeLa Radical Scavenging Activity Total Phenolic Content |
title_short |
Macroalgae from S. Miguel Island as a potential source of antiproliferative and antioxidant products |
title_full |
Macroalgae from S. Miguel Island as a potential source of antiproliferative and antioxidant products |
title_fullStr |
Macroalgae from S. Miguel Island as a potential source of antiproliferative and antioxidant products |
title_full_unstemmed |
Macroalgae from S. Miguel Island as a potential source of antiproliferative and antioxidant products |
title_sort |
Macroalgae from S. Miguel Island as a potential source of antiproliferative and antioxidant products |
author |
Barreto, Maria do Carmo |
author_facet |
Barreto, Maria do Carmo Mendonça, Emanuel A. Gouveia, Vera F. Anjos, Carolina Medeiros, Joana S. Seca, Ana M. L. Neto, Ana I. |
author_role |
author |
author2 |
Mendonça, Emanuel A. Gouveia, Vera F. Anjos, Carolina Medeiros, Joana S. Seca, Ana M. L. Neto, Ana I. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade dos Açores |
dc.contributor.author.fl_str_mv |
Barreto, Maria do Carmo Mendonça, Emanuel A. Gouveia, Vera F. Anjos, Carolina Medeiros, Joana S. Seca, Ana M. L. Neto, Ana I. |
dc.subject.por.fl_str_mv |
Azores Marine Macroalgae Cytotoxicity HeLa Radical Scavenging Activity Total Phenolic Content |
topic |
Azores Marine Macroalgae Cytotoxicity HeLa Radical Scavenging Activity Total Phenolic Content |
description |
Ulva compressa, Gelidium microdon, Osmundea pinnatifida, Fucus spiralis and Cystoseira abies-marina from the coast of S. Miguel Island were screened for their in vitro cytotoxicity against HeLa tumour cell line, antioxidant potential and total phenolic content. From each alga, the hexane fraction (HF) of the methanol extract, the methanol residue (MF) and the dichloromethane extract (DE), were obtained and evaluated. The highest antiproliferative activity against HeLa cell line was found in C. abies-marina and F. spiralis DE (IC50 8.8 μg/mL and 10.7 μg/mL, respectively), and presented selective cytotoxicity, when tested against Vero cell line. Fluorescence microscopy studies of the two most active extracts suggest an apoptosis-inducing activity. Concerning the antioxidant activity, high values were found on F. spiralis MF and HF (EC50 0.62 μg/mL and 2.01 μg/mL, respectively), even higher than those obtained for trolox and quercetin. This high antioxidant activity in F. spiralis MF can be explained by its content in phenolic compounds, but not for HF, where the antioxidant compounds must be less polar. These results suggest that some macroalgae from the Azorean sea have great potential which could be considered for future applications in medicine, food production or cosmetics industry. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2012-01-01T00:00:00Z 2013-01-09T13:01:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.3/1556 |
url |
http://hdl.handle.net/10400.3/1556 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"ARQUIPÉLAGO. Life and Marine Sciences". ISSN 0873-4704. Nº 29 (2012): 53-58 0873-4704 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade dos Açores |
publisher.none.fl_str_mv |
Universidade dos Açores |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130689702985728 |