Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/1405 |
Resumo: | Tese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de Ciências |
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Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissuesBiologia celularApoptoseCélulas epiteliaisTeses de mestradoTese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de CiênciasThe actin cytoskeleton has a central role in controlling cell shape and mobility. In epithelia, a circumferential band of actin filaments provides the structural support for cell-cell junctions. When the strength of the epithelium is compromised, cells may undergo epithelium to mesenchymal transition, escape size-control mechanisms, evade cell death and finally acquire the ability to migrate. These features recapitulate all of the hallmarks that characterize cancer malignancy. Interestingly, clones of cells mutant for either subunits of the capping protein ab heterodimer (CP), induced in a heterozygous wild-type background, are extruded from the wing blade epithelium and die. However, depleting the subunit (cpb) by RNA interference (RNAi) in the whole blade leads to a different outcome: epithelium polarity is strongly affected, few apoptotic cells can be observed, while many seem to overproliferate. This differential behavior is unlikely to be due to a dosage effect since RNAi induced-cpb depletion in restricted wing blade domains also induces cell extrusion and death. This suggests that CP prevents tumorigenesis of wing blade cells. However, when CP mutant cells are adjacent to wildtype wing blade neighboring cells, the latest eliminate mutant cells by a process of cell competition. The role of CP in preventing tumorigenesis might be related to its major function, preventing excessive actin polimerization, or to additional functions, such as the maintenance of epithelial cell polarity. The tumor suppressor function of CP appears to be tissue specific since the above cell behavior is only observed in restricted epithelia. This suggests that each epithelium has specific cytoskeleton and/or junctional properties, making cells sensitive or not to mutations that cause abnormal tumor growth. Altogether the presented data highlight the crucial impact of tissue context for the activation of a tumoral processResumo alargado em português disponível no documentoJanody, FlorenceRodrigues, Maria GabrielaRepositório da Universidade de LisboaRebelo, Sofia Raquel Paulo2010-07-27T08:58:04Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdftext/xmlhttp://hdl.handle.net/10451/1405enghttp://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000568794info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:40:54Zoai:repositorio.ul.pt:10451/1405Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:28:03.265890Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues |
title |
Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues |
spellingShingle |
Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues Rebelo, Sofia Raquel Paulo Biologia celular Apoptose Células epiteliais Teses de mestrado |
title_short |
Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues |
title_full |
Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues |
title_fullStr |
Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues |
title_full_unstemmed |
Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues |
title_sort |
Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues |
author |
Rebelo, Sofia Raquel Paulo |
author_facet |
Rebelo, Sofia Raquel Paulo |
author_role |
author |
dc.contributor.none.fl_str_mv |
Janody, Florence Rodrigues, Maria Gabriela Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Rebelo, Sofia Raquel Paulo |
dc.subject.por.fl_str_mv |
Biologia celular Apoptose Células epiteliais Teses de mestrado |
topic |
Biologia celular Apoptose Células epiteliais Teses de mestrado |
description |
Tese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de Ciências |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z 2010-07-27T08:58:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/1405 |
url |
http://hdl.handle.net/10451/1405 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000568794 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf text/xml |
dc.source.none.fl_str_mv |
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instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134162899173376 |