Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidis

Detalhes bibliográficos
Autor(a) principal: Cerca, Nuno
Data de Publicação: 2003
Outros Autores: Pier, Gerald B., Oliveira, Rosário, Azeredo, Joana
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/4680
Resumo: Staphylococcus epidermidis and similar coagulase-negative staphylococci (CoNS) are now well established as major nosocomial pathogens associated with infections of indwelling medical devices. The major virulence factor of these organisms is mainly due to their ability to adhere to devices and form a biofilm.however, it is not known if adherence and biofilm formation are closely linked phenotypes for clinical isolates. since different clinical isolates of s. epidermidis would be expected to exhibit different phenotypic behaviours it is further expected that strains of s. epidermidis might have different abilities to adhere to synthetic surfaces and subsequently produce biofilms. in this study the initial adherence and subsequent biofilm formation properties of 9 clinical isolates of s. epidermidis along with biofilm+ and biofilm– control strains were assayed. the adherence results were interpreted in terms of the physico-chemical interaction established between the cells and the adhesion substratum as assessed by contact angle measurements.as expected, the clinical isolates exhibited different abilities to adhere to hydrophilic glass and to form biofilms. moreover, the strains that produced the highest amounts of biofilms were not the ones able to adhere to the largest extent and vice-versa. for example, the biofilm- control strain actually showed the highest level of initial adhesion capability and did not produce biofilm. these results indicate that high levels of initial adherence do not necessarily lead to strong biofilm formation and that some strains do not have a high initial adherence but can subsequently form a strong biofilm. these two aspects of the pathogenesis of medical device related-infection may need to be evaluated independently to ascertain the contribution of each to the virulence of cons causing device related infections.
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spelling Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidisStaphylococcus epidermidis and similar coagulase-negative staphylococci (CoNS) are now well established as major nosocomial pathogens associated with infections of indwelling medical devices. The major virulence factor of these organisms is mainly due to their ability to adhere to devices and form a biofilm.however, it is not known if adherence and biofilm formation are closely linked phenotypes for clinical isolates. since different clinical isolates of s. epidermidis would be expected to exhibit different phenotypic behaviours it is further expected that strains of s. epidermidis might have different abilities to adhere to synthetic surfaces and subsequently produce biofilms. in this study the initial adherence and subsequent biofilm formation properties of 9 clinical isolates of s. epidermidis along with biofilm+ and biofilm– control strains were assayed. the adherence results were interpreted in terms of the physico-chemical interaction established between the cells and the adhesion substratum as assessed by contact angle measurements.as expected, the clinical isolates exhibited different abilities to adhere to hydrophilic glass and to form biofilms. moreover, the strains that produced the highest amounts of biofilms were not the ones able to adhere to the largest extent and vice-versa. for example, the biofilm- control strain actually showed the highest level of initial adhesion capability and did not produce biofilm. these results indicate that high levels of initial adherence do not necessarily lead to strong biofilm formation and that some strains do not have a high initial adherence but can subsequently form a strong biofilm. these two aspects of the pathogenesis of medical device related-infection may need to be evaluated independently to ascertain the contribution of each to the virulence of cons causing device related infections.American Society for MicrobiologyUniversidade do MinhoCerca, NunoPier, Gerald B.Oliveira, RosárioAzeredo, Joana20032003-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/4680engASM CONFERENCE ON BIOFILMS, Victoria, 2003 - "ASM Conference on Biofilms". Victoria : American Society of Microbiology, 2003. 1-55581289-9. p. 163.1-55581289-9info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:07:39Zoai:repositorium.sdum.uminho.pt:1822/4680Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:07:39Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidis
title Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidis
spellingShingle Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidis
Cerca, Nuno
title_short Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidis
title_full Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidis
title_fullStr Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidis
title_full_unstemmed Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidis
title_sort Differences in adhesion and biofilm formation of several clinical isolates of Staphylococcus epidermidis
author Cerca, Nuno
author_facet Cerca, Nuno
Pier, Gerald B.
Oliveira, Rosário
Azeredo, Joana
author_role author
author2 Pier, Gerald B.
Oliveira, Rosário
Azeredo, Joana
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Cerca, Nuno
Pier, Gerald B.
Oliveira, Rosário
Azeredo, Joana
description Staphylococcus epidermidis and similar coagulase-negative staphylococci (CoNS) are now well established as major nosocomial pathogens associated with infections of indwelling medical devices. The major virulence factor of these organisms is mainly due to their ability to adhere to devices and form a biofilm.however, it is not known if adherence and biofilm formation are closely linked phenotypes for clinical isolates. since different clinical isolates of s. epidermidis would be expected to exhibit different phenotypic behaviours it is further expected that strains of s. epidermidis might have different abilities to adhere to synthetic surfaces and subsequently produce biofilms. in this study the initial adherence and subsequent biofilm formation properties of 9 clinical isolates of s. epidermidis along with biofilm+ and biofilm– control strains were assayed. the adherence results were interpreted in terms of the physico-chemical interaction established between the cells and the adhesion substratum as assessed by contact angle measurements.as expected, the clinical isolates exhibited different abilities to adhere to hydrophilic glass and to form biofilms. moreover, the strains that produced the highest amounts of biofilms were not the ones able to adhere to the largest extent and vice-versa. for example, the biofilm- control strain actually showed the highest level of initial adhesion capability and did not produce biofilm. these results indicate that high levels of initial adherence do not necessarily lead to strong biofilm formation and that some strains do not have a high initial adherence but can subsequently form a strong biofilm. these two aspects of the pathogenesis of medical device related-infection may need to be evaluated independently to ascertain the contribution of each to the virulence of cons causing device related infections.
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/4680
url http://hdl.handle.net/1822/4680
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv ASM CONFERENCE ON BIOFILMS, Victoria, 2003 - "ASM Conference on Biofilms". Victoria : American Society of Microbiology, 2003. 1-55581289-9. p. 163.
1-55581289-9
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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