A simple cultural method for the presumptive detection of the yeasts Brettanomyces/Dekkera in wines
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/6723 |
Resumo: | The development of a simple and reliable procedure, compatible with routine use in wineries, for the presumptive detection of Brettanomyces/Dekkera from wine and wine-environment samples. Methods and Results: The method of detection of these yeasts employs a selective enrichment medium. The medium contains glucose (10 g l⁻¹1) as carbon and energy source, cycloheximide (20 mg l⁻¹1) to prevent growth of Saccharomyces, chloramphenicol (200 mg l⁻¹1) to prevent growth of bacteria and p-coumaric acid (20 mg l⁻¹1) as the precursor for the production of 4-ethyl-phenol. After the inoculation with wine, the medium is monitored by visual inspection of turbidity and by periodic olfactive analysis. Contaminated wines will develop visible turbidity in the medium and will produce the 4-ethyl-phenol off-odour, which can be easily detected by smelling. Conclusions: A selective enrichment liquid medium was developed to differentially promote the growth and activity of Brettanomyces/Dekkera. The method is simple to execute, employing a simple-to-prepare medium and a periodic olfactive detection. Significance and Impact of the Study: The characteristics of the procedure make it particularly applicable in a wine-making environment thus presenting important advantages to the wine industry. |
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A simple cultural method for the presumptive detection of the yeasts Brettanomyces/Dekkera in winesBrettanomyces/DekkeraDetectionVolatile phenolsWineWine industryThe development of a simple and reliable procedure, compatible with routine use in wineries, for the presumptive detection of Brettanomyces/Dekkera from wine and wine-environment samples. Methods and Results: The method of detection of these yeasts employs a selective enrichment medium. The medium contains glucose (10 g l⁻¹1) as carbon and energy source, cycloheximide (20 mg l⁻¹1) to prevent growth of Saccharomyces, chloramphenicol (200 mg l⁻¹1) to prevent growth of bacteria and p-coumaric acid (20 mg l⁻¹1) as the precursor for the production of 4-ethyl-phenol. After the inoculation with wine, the medium is monitored by visual inspection of turbidity and by periodic olfactive analysis. Contaminated wines will develop visible turbidity in the medium and will produce the 4-ethyl-phenol off-odour, which can be easily detected by smelling. Conclusions: A selective enrichment liquid medium was developed to differentially promote the growth and activity of Brettanomyces/Dekkera. The method is simple to execute, employing a simple-to-prepare medium and a periodic olfactive detection. Significance and Impact of the Study: The characteristics of the procedure make it particularly applicable in a wine-making environment thus presenting important advantages to the wine industry.WileyVeritati - Repositório Institucional da Universidade Católica PortuguesaCouto, J. A.Barbosa, A.Hogg, T.2011-10-21T15:40:29Z20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6723eng"Letters in Applied Microbiology". ISSN 0266-8254. 41 (2005) 505–510info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:09:22Zoai:repositorio.ucp.pt:10400.14/6723Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:05:01.227840Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A simple cultural method for the presumptive detection of the yeasts Brettanomyces/Dekkera in wines |
title |
A simple cultural method for the presumptive detection of the yeasts Brettanomyces/Dekkera in wines |
spellingShingle |
A simple cultural method for the presumptive detection of the yeasts Brettanomyces/Dekkera in wines Couto, J. A. Brettanomyces/Dekkera Detection Volatile phenols Wine Wine industry |
title_short |
A simple cultural method for the presumptive detection of the yeasts Brettanomyces/Dekkera in wines |
title_full |
A simple cultural method for the presumptive detection of the yeasts Brettanomyces/Dekkera in wines |
title_fullStr |
A simple cultural method for the presumptive detection of the yeasts Brettanomyces/Dekkera in wines |
title_full_unstemmed |
A simple cultural method for the presumptive detection of the yeasts Brettanomyces/Dekkera in wines |
title_sort |
A simple cultural method for the presumptive detection of the yeasts Brettanomyces/Dekkera in wines |
author |
Couto, J. A. |
author_facet |
Couto, J. A. Barbosa, A. Hogg, T. |
author_role |
author |
author2 |
Barbosa, A. Hogg, T. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Couto, J. A. Barbosa, A. Hogg, T. |
dc.subject.por.fl_str_mv |
Brettanomyces/Dekkera Detection Volatile phenols Wine Wine industry |
topic |
Brettanomyces/Dekkera Detection Volatile phenols Wine Wine industry |
description |
The development of a simple and reliable procedure, compatible with routine use in wineries, for the presumptive detection of Brettanomyces/Dekkera from wine and wine-environment samples. Methods and Results: The method of detection of these yeasts employs a selective enrichment medium. The medium contains glucose (10 g l⁻¹1) as carbon and energy source, cycloheximide (20 mg l⁻¹1) to prevent growth of Saccharomyces, chloramphenicol (200 mg l⁻¹1) to prevent growth of bacteria and p-coumaric acid (20 mg l⁻¹1) as the precursor for the production of 4-ethyl-phenol. After the inoculation with wine, the medium is monitored by visual inspection of turbidity and by periodic olfactive analysis. Contaminated wines will develop visible turbidity in the medium and will produce the 4-ethyl-phenol off-odour, which can be easily detected by smelling. Conclusions: A selective enrichment liquid medium was developed to differentially promote the growth and activity of Brettanomyces/Dekkera. The method is simple to execute, employing a simple-to-prepare medium and a periodic olfactive detection. Significance and Impact of the Study: The characteristics of the procedure make it particularly applicable in a wine-making environment thus presenting important advantages to the wine industry. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 2005-01-01T00:00:00Z 2011-10-21T15:40:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/6723 |
url |
http://hdl.handle.net/10400.14/6723 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Letters in Applied Microbiology". ISSN 0266-8254. 41 (2005) 505–510 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131712906592256 |