Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes

Detalhes bibliográficos
Autor(a) principal: Mira, Ana Rita
Data de Publicação: 2023
Outros Autores: Zeferino, Ana Sofia, Inácio, Raquel, Delgadinho, Mariana, Brito, Miguel, Calado, Cecília R. C., Ribeiro, Edna
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/15932
Resumo: Projeto “Resistance modulation and epigenetic divergence in resistant phenotypic profiles of Staphylococcus aureus” (IPL/2020/ EpiResistanceSA _ESTeSL).
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spelling Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypesDrug resistance modulatorsDrug resistance phenotypesEpigallocatechin-3-gallateEGCGEpigenetic modulatorHealthcare-associated S. aureus infectionsSynergistic effectsIPL/2020/ EpiResistanceSA _ESTeSLFCT_UIDB/05608/2020FCT_UIDP/05608/2020Projeto “Resistance modulation and epigenetic divergence in resistant phenotypic profiles of Staphylococcus aureus” (IPL/2020/ EpiResistanceSA _ESTeSL).FCT_UIDB/05608/2020. FCT_UIDP/05608/2020.Healthcare-associated methicillin-resistant Staphylococcus aureus infections represent extremely high morbidity and mortality rates worldwide. We aimed to assess the antimicrobial potential and synergistic effect between Epigalocatenin-3-gallate (EGCG) and different antibiotics in S. aureus strains with divergent resistance phenotypes. EGCG exposure effects in epigenetic and drug resistance key modulators were also evaluated. S. aureus strains (n = 32) were isolated from infected patients in a Lisbon hospital. The identification of the S. aureus resistance phenotype was performed through automatized methods. The antibiotic synergistic assay was performed through disk diffusion according to EUCAST guidelines with co-exposure to EGCG (250, 100, 50, and 25 µg/mL). The bacteria's molecular profile was assessed through FTIR spectroscopy. The transcriptional expression of OrfX, SpdC, and WalKR was performed by using qRT-PCR. FTIR-spectroscopy analysis enabled the clear discrimination of MRSA/MSSA strains and the EGCG exposure effect in the bacteria's molecular profiles. Divergent resistant phenotypes were associated with divergent transcriptional expression of the epigenetic modulator OrfX, particularly in MRSA strains, as well as the key drug response modulators SpdC and WalKR. These results clearly demonstrate that EGCG exposure alters the expression patterns of key epigenetic and drug response genes with associated divergent-resistant profiles, which supports its potential for antimicrobial treatment and/or therapeutic adjuvant against antibiotic-resistant microorganisms.MDPIRCIPLMira, Ana RitaZeferino, Ana SofiaInácio, RaquelDelgadinho, MarianaBrito, MiguelCalado, Cecília R. C.Ribeiro, Edna2023-04-21T17:55:26Z2023-032023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/15932engMira AR, Zeferino AS, Inácio R, Delgadinho M, Brito M, Calado CR, Ribeiro E. Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes. Antibiotics. 2023;12(3):519.10.3390/antibiotics12030519info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:14:02Zoai:repositorio.ipl.pt:10400.21/15932Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:23:31.102167Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes
title Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes
spellingShingle Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes
Mira, Ana Rita
Drug resistance modulators
Drug resistance phenotypes
Epigallocatechin-3-gallate
EGCG
Epigenetic modulator
Healthcare-associated S. aureus infections
Synergistic effects
IPL/2020/ EpiResistanceSA _ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
title_short Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes
title_full Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes
title_fullStr Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes
title_full_unstemmed Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes
title_sort Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes
author Mira, Ana Rita
author_facet Mira, Ana Rita
Zeferino, Ana Sofia
Inácio, Raquel
Delgadinho, Mariana
Brito, Miguel
Calado, Cecília R. C.
Ribeiro, Edna
author_role author
author2 Zeferino, Ana Sofia
Inácio, Raquel
Delgadinho, Mariana
Brito, Miguel
Calado, Cecília R. C.
Ribeiro, Edna
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Mira, Ana Rita
Zeferino, Ana Sofia
Inácio, Raquel
Delgadinho, Mariana
Brito, Miguel
Calado, Cecília R. C.
Ribeiro, Edna
dc.subject.por.fl_str_mv Drug resistance modulators
Drug resistance phenotypes
Epigallocatechin-3-gallate
EGCG
Epigenetic modulator
Healthcare-associated S. aureus infections
Synergistic effects
IPL/2020/ EpiResistanceSA _ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
topic Drug resistance modulators
Drug resistance phenotypes
Epigallocatechin-3-gallate
EGCG
Epigenetic modulator
Healthcare-associated S. aureus infections
Synergistic effects
IPL/2020/ EpiResistanceSA _ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
description Projeto “Resistance modulation and epigenetic divergence in resistant phenotypic profiles of Staphylococcus aureus” (IPL/2020/ EpiResistanceSA _ESTeSL).
publishDate 2023
dc.date.none.fl_str_mv 2023-04-21T17:55:26Z
2023-03
2023-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/15932
url http://hdl.handle.net/10400.21/15932
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mira AR, Zeferino AS, Inácio R, Delgadinho M, Brito M, Calado CR, Ribeiro E. Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes. Antibiotics. 2023;12(3):519.
10.3390/antibiotics12030519
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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