The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium

Detalhes bibliográficos
Autor(a) principal: Amaral, Ana
Data de Publicação: 2020
Outros Autores: Fernandes, Carina, Rebordão, Maria Rosa, Szóstek-Mioduchowska, Anna, Lukasik, Karolina, Gawronska-Kozak, Barbara, Gama, Luis, Skarzynski, Dariusz J., Ferreira-Dias, Graça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/24097
Resumo: Research Areas: Agriculture ; Veterinary Sciences ; Zoology
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spelling The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometriumEndometrosisMareElastaseSivelestatCollagenMetallopeptidasesEndometriumNeutrophil extracellular traps (NETs)Research Areas: Agriculture ; Veterinary Sciences ; ZoologyAbstract: Neutrophil extracellular traps (NETs) fight endometritis, and elastase (ELA), a protease found in NETs, might induce collagen type I (COL1) accumulation in equine endometrium. Metallopeptidases (MMPs) are involved in extracellular matrix balance. The aim was to evaluate the e ects of ELA and sivelestat (selective elastase inhibitor) on MMP-2 and MMP-9 expression and gelatinolytic activity, as well as the potential inhibitory e ect of sivelestat on ELA-induced COL1 in equine endometrium. Endometrial explants from follicular (FP) and mid-luteal (MLP) phases were treated for 24 or 48 h with ELA, sivelestat, and their combination. Transcripts of COL1A2, MMP2, and MMP9 were evaluated by qPCR; COL1 protein relative abundance by Western blot, and MMP-2 and MMP-9 gelatinolytic activity by zymography. In response to ELA treatment, there was an increase in MMP2 mRNA transcription (24 h) in active MMP-2 (48 h), both in FP, and in MMP9 transcripts in FP (48 h) and MLP (24 h) (p < 0.05). Sivelestat inhibited ELA-induced COL1A2 transcripts in FP (24 h) and MLP (24 h, 48 h) (p < 0.05). The sivelestat inhibitory e ect was detected in MMP9 transcripts in FP at 48 h (p < 0.05), but proteases activity was unchanged. Thus, MMP-2 and MMP-9 might be implicated in endometrium fibrotic response to ELA. In mare endometrium, sivelestat may decrease ELA-induced COL1 deposition and hinder endometrosis development.MDPIRepositório da Universidade de LisboaAmaral, AnaFernandes, CarinaRebordão, Maria RosaSzóstek-Mioduchowska, AnnaLukasik, KarolinaGawronska-Kozak, BarbaraGama, LuisSkarzynski, Dariusz J.Ferreira-Dias, Graça2022-04-18T14:07:04Z2020-05-162020-05-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24097engAmaral A, Fernandes C, Rebordao MR, Szostek-Mioduchowska A, Lukasik K, Lukasik K, Gawronska-Kozak B, Gama LT, Skarzynski DJ, Ferreira-Dias, G . 2020. The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium. Animals, 10(5):863. Doi: 10.3390/ani100508632076-261510.3390/ani10050863info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:44Zoai:www.repository.utl.pt:10400.5/24097Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:13.099194Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium
title The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium
spellingShingle The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium
Amaral, Ana
Endometrosis
Mare
Elastase
Sivelestat
Collagen
Metallopeptidases
Endometrium
Neutrophil extracellular traps (NETs)
title_short The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium
title_full The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium
title_fullStr The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium
title_full_unstemmed The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium
title_sort The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium
author Amaral, Ana
author_facet Amaral, Ana
Fernandes, Carina
Rebordão, Maria Rosa
Szóstek-Mioduchowska, Anna
Lukasik, Karolina
Gawronska-Kozak, Barbara
Gama, Luis
Skarzynski, Dariusz J.
Ferreira-Dias, Graça
author_role author
author2 Fernandes, Carina
Rebordão, Maria Rosa
Szóstek-Mioduchowska, Anna
Lukasik, Karolina
Gawronska-Kozak, Barbara
Gama, Luis
Skarzynski, Dariusz J.
Ferreira-Dias, Graça
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Amaral, Ana
Fernandes, Carina
Rebordão, Maria Rosa
Szóstek-Mioduchowska, Anna
Lukasik, Karolina
Gawronska-Kozak, Barbara
Gama, Luis
Skarzynski, Dariusz J.
Ferreira-Dias, Graça
dc.subject.por.fl_str_mv Endometrosis
Mare
Elastase
Sivelestat
Collagen
Metallopeptidases
Endometrium
Neutrophil extracellular traps (NETs)
topic Endometrosis
Mare
Elastase
Sivelestat
Collagen
Metallopeptidases
Endometrium
Neutrophil extracellular traps (NETs)
description Research Areas: Agriculture ; Veterinary Sciences ; Zoology
publishDate 2020
dc.date.none.fl_str_mv 2020-05-16
2020-05-16T00:00:00Z
2022-04-18T14:07:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/24097
url http://hdl.handle.net/10400.5/24097
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Amaral A, Fernandes C, Rebordao MR, Szostek-Mioduchowska A, Lukasik K, Lukasik K, Gawronska-Kozak B, Gama LT, Skarzynski DJ, Ferreira-Dias, G . 2020. The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium. Animals, 10(5):863. Doi: 10.3390/ani10050863
2076-2615
10.3390/ani10050863
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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