An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-care
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/150359 |
Resumo: | Funding Information: The authors acknowledge funding through project TecniCov ( POCI-01-02B7-FEDER-069745 ), co-funded by FEDER through COMPETE2020 and Lisboa2020 and CY-Sensors ( POCI-01-0145-FEDER-032359 ) through Fundação para a Ciência e a Tecnologia (FCT) , Portugal. Publisher Copyright: © 2022 The Authors |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-careAntibodies for SARS-CoV-2Electrochemical biosensorPoint-of-careProtective immunitySARS-CoV-2Spike proteinBiotechnologyBioengineeringBiomaterialsBiomedical EngineeringMolecular BiologyCell BiologySDG 3 - Good Health and Well-beingFunding Information: The authors acknowledge funding through project TecniCov ( POCI-01-02B7-FEDER-069745 ), co-funded by FEDER through COMPETE2020 and Lisboa2020 and CY-Sensors ( POCI-01-0145-FEDER-032359 ) through Fundação para a Ciência e a Tecnologia (FCT) , Portugal. Publisher Copyright: © 2022 The AuthorsThis work presents an innovative ultra-sensitive biosensor having the Spike protein on carbon-based screen-printed electrodes (SPEs), for monitoring in point-of-care antibodies against SARS-CoV-2, a very important tool for epidemiological monitoring of COVID-19 infection and establishing vaccination schemes. In an innovative and simple approach, a highly conductive support is combined with the direct adsorption of Spike protein to enable an extensive antibody capture. The high conductivity was ensured by using carboxylated carbon nanotubes on the carbon electrode, by means of a simple and quick approach, which also increased the surface area. These were then modified with EDC/NHS chemistry to produce an amine layer and undergo Spike protein adsorption, to generate a stable layer capable of capturing the antibodies against SARS-CoV-2 in serum with great sensitivity. Electrochemical impedance spectroscopy was used to evaluate the analytical performance of this biosensor in serum. It displayed a linear response between 1.0 pg/mL and 10 ng/mL, with a detection limit of ∼0.7 pg/mL. The analysis of human positive sera containing antibody in a wide range of concentrations yielded accurate data, correlating well with the reference method. It also offered the unique ability of discriminating antibody concentrations in sera below 2.3 μg/mL, the lowest value detected by the commercial method. In addition, a proof-of-concept study was performed by labelling anti-IgG antibodies with quantum dots to explore a new electrochemical readout based on the signal generated upon binding to the anti-S protein antibodies recognised on the surface of the biosensor. Overall, the alternative serologic assay presented is a promising tool for assessing protective immunity to SARS-CoV-2 and a potential guide for revaccination.CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisUNINOVA-Instituto de Desenvolvimento de Novas TecnologiasRUNCardoso, Ana R.Alves, João FredericoFrasco, Manuela F.Piloto, Ana MargaridaSerrano, VerónicaMateus, DanielaSebastião, Ana IsabelMatos, Ana MiguelCarmo, AnáliaCruz, TeresaFortunato, ElviraSales, M. Goreti F.2023-03-10T22:54:24Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/150359eng2590-0064PURE: 55265736https://doi.org/10.1016/j.mtbio.2022.100354info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:09:50Zoai:run.unl.pt:10362/150359Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:09:50Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-care |
| title |
An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-care |
| spellingShingle |
An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-care Cardoso, Ana R. Antibodies for SARS-CoV-2 Electrochemical biosensor Point-of-care Protective immunity SARS-CoV-2 Spike protein Biotechnology Bioengineering Biomaterials Biomedical Engineering Molecular Biology Cell Biology SDG 3 - Good Health and Well-being |
| title_short |
An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-care |
| title_full |
An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-care |
| title_fullStr |
An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-care |
| title_full_unstemmed |
An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-care |
| title_sort |
An ultra-sensitive electrochemical biosensor using the Spike protein for capturing antibodies against SARS-CoV-2 in point-of-care |
| author |
Cardoso, Ana R. |
| author_facet |
Cardoso, Ana R. Alves, João Frederico Frasco, Manuela F. Piloto, Ana Margarida Serrano, Verónica Mateus, Daniela Sebastião, Ana Isabel Matos, Ana Miguel Carmo, Anália Cruz, Teresa Fortunato, Elvira Sales, M. Goreti F. |
| author_role |
author |
| author2 |
Alves, João Frederico Frasco, Manuela F. Piloto, Ana Margarida Serrano, Verónica Mateus, Daniela Sebastião, Ana Isabel Matos, Ana Miguel Carmo, Anália Cruz, Teresa Fortunato, Elvira Sales, M. Goreti F. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) DCM - Departamento de Ciência dos Materiais UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias RUN |
| dc.contributor.author.fl_str_mv |
Cardoso, Ana R. Alves, João Frederico Frasco, Manuela F. Piloto, Ana Margarida Serrano, Verónica Mateus, Daniela Sebastião, Ana Isabel Matos, Ana Miguel Carmo, Anália Cruz, Teresa Fortunato, Elvira Sales, M. Goreti F. |
| dc.subject.por.fl_str_mv |
Antibodies for SARS-CoV-2 Electrochemical biosensor Point-of-care Protective immunity SARS-CoV-2 Spike protein Biotechnology Bioengineering Biomaterials Biomedical Engineering Molecular Biology Cell Biology SDG 3 - Good Health and Well-being |
| topic |
Antibodies for SARS-CoV-2 Electrochemical biosensor Point-of-care Protective immunity SARS-CoV-2 Spike protein Biotechnology Bioengineering Biomaterials Biomedical Engineering Molecular Biology Cell Biology SDG 3 - Good Health and Well-being |
| description |
Funding Information: The authors acknowledge funding through project TecniCov ( POCI-01-02B7-FEDER-069745 ), co-funded by FEDER through COMPETE2020 and Lisboa2020 and CY-Sensors ( POCI-01-0145-FEDER-032359 ) through Fundação para a Ciência e a Tecnologia (FCT) , Portugal. Publisher Copyright: © 2022 The Authors |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-12 2022-12-01T00:00:00Z 2023-03-10T22:54:24Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/150359 |
| url |
http://hdl.handle.net/10362/150359 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2590-0064 PURE: 55265736 https://doi.org/10.1016/j.mtbio.2022.100354 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
10 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545921331724288 |