Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building

Detalhes bibliográficos
Autor(a) principal: David, Susana
Data de Publicação: 2012
Outros Autores: Sutre, AF, Sanca, A, Mané, A, Henriques, V, Portugal, Clara, Sancho, Luísa, Cardoso, A, Paixão, Eleonora, Duarte, EL, Leite, CQF, Salem, JI, Antunes, Abílio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/7228
https://doi.org/10.5588/ijtld.11.0658
Resumo: The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement,heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
id RCAP_79fa46406b408f948b5a8045eb9555e3
oai_identifier_str oai:dspace.uevora.pt:10174/7228
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity buildingMycobacterium tuberculosisrpoBsputum smear microscopysodium hypochloriteDHPLC sequencingThe diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement,heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.The Union2013-01-14T10:25:57Z2013-01-142012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/7228http://hdl.handle.net/10174/7228https://doi.org/10.5588/ijtld.11.0658engDavid, Susana; Sutre AF; Sanca A; Mané A; Henriques V; Portugal C; Sancho L; Cardoso A; Paixão, Eleonora; Duarte, EL; Leite CQF; Salem JI; Antunes A. Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building, International Journal of Tuberculosis and Lung Disease , 16, 11, 1535-1537, 2012.MVTndndndndndndndndndndndndnd546David, SusanaSutre, AFSanca, AMané, AHenriques, VPortugal, ClaraSancho, LuísaCardoso, APaixão, EleonoraDuarte, ELLeite, CQFSalem, JIAntunes, Abílioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:47:16Zoai:dspace.uevora.pt:10174/7228Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:01:48.338103Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
title Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
spellingShingle Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
David, Susana
Mycobacterium tuberculosis
rpoB
sputum smear microscopy
sodium hypochlorite
DHPLC sequencing
title_short Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
title_full Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
title_fullStr Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
title_full_unstemmed Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
title_sort Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
author David, Susana
author_facet David, Susana
Sutre, AF
Sanca, A
Mané, A
Henriques, V
Portugal, Clara
Sancho, Luísa
Cardoso, A
Paixão, Eleonora
Duarte, EL
Leite, CQF
Salem, JI
Antunes, Abílio
author_role author
author2 Sutre, AF
Sanca, A
Mané, A
Henriques, V
Portugal, Clara
Sancho, Luísa
Cardoso, A
Paixão, Eleonora
Duarte, EL
Leite, CQF
Salem, JI
Antunes, Abílio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv David, Susana
Sutre, AF
Sanca, A
Mané, A
Henriques, V
Portugal, Clara
Sancho, Luísa
Cardoso, A
Paixão, Eleonora
Duarte, EL
Leite, CQF
Salem, JI
Antunes, Abílio
dc.subject.por.fl_str_mv Mycobacterium tuberculosis
rpoB
sputum smear microscopy
sodium hypochlorite
DHPLC sequencing
topic Mycobacterium tuberculosis
rpoB
sputum smear microscopy
sodium hypochlorite
DHPLC sequencing
description The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement,heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01T00:00:00Z
2013-01-14T10:25:57Z
2013-01-14
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/7228
http://hdl.handle.net/10174/7228
https://doi.org/10.5588/ijtld.11.0658
url http://hdl.handle.net/10174/7228
https://doi.org/10.5588/ijtld.11.0658
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv David, Susana; Sutre AF; Sanca A; Mané A; Henriques V; Portugal C; Sancho L; Cardoso A; Paixão, Eleonora; Duarte, EL; Leite CQF; Salem JI; Antunes A. Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building, International Journal of Tuberculosis and Lung Disease , 16, 11, 1535-1537, 2012.
MVT
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
546
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv The Union
publisher.none.fl_str_mv The Union
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799136501678735360

Registros relacionados