Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate

Detalhes bibliográficos
Autor(a) principal: Meneses, D.
Data de Publicação: 2021
Outros Autores: Paixão, L., Fonteles, Thatyane V., Gudiña, Eduardo José, Rodrigues, L. R., Fernandes, Fabiano A. N., Rodrigues, Sueli
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/70362
Resumo: Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.bej.2021.107954.
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spelling Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrateesteraseAureobasidium pullulans URM 7059airlift reactorbiodegradationScience & TechnologySupplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.bej.2021.107954.Aureobasidium pullulans URM 7059 produced esterase using residual glycerol from biodiesel as the sole carbon source. The culture medium containing residual glycerol (0.1% v/v), (NH4)2SO4 (4g/L), and yeast extract (8g/L) resulted in the highest esterase production using shake-flasks. The enzyme exhibited a molar mass of 50kDa and was stable at neutral pH and temperatures below 30°C. The cations Cu2+ and Al3+ did not affect the esterase activity, while Ca2+ promoted the highest activity loss. The enzyme kinetic parameters were determined using different substrates (p-nitrophenylcaprylate and p-nitrophenylbutyrate). Km and Vmax were 1.4mM and 218µmolmin-1 for p-NPC, and 1.55mM and 76.7µmolmin-1 for p-NPB. The esterase production was further evaluated using stirred tank and 2-L airlift bioreactors. The airlift reactor operating at the highest air flow rate (8L/min) increased the enzyme productivity 3-fold compared to the shake-flasks experiments. However, the crude enzymatic extract showed 3 active protein bands by zymography with molecular masses of 172kDa, 66kDa, and 40kDa approximately, suggesting that the pattern of enzyme production changed due to aeration. The crude enzyme degraded the MACO-Sty biopolymer in 14 days, being stable in a wide range of pH (7.0 9.0) and temperatures (40°C 80°C). The results suggest that this enzyme is a promising catalyst in remediation processes.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004), projects MultiBiorefinery (POCI-01-0145-FEDER016403) and Lignozymes (POCI-01-0145-FEDER-029773), all funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. In Brazil, this study was financed in part by the Coordenaçao ˜ de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The authors also thank the CNPq and FUNCAP for financial support.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoMeneses, D.Paixão, L.Fonteles, Thatyane V.Gudiña, Eduardo JoséRodrigues, L. R.Fernandes, Fabiano A. N.Rodrigues, Sueli2021-042021-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/70362engMeneses, D.; Paixão, L.; Fonteles, Thatyane V.; Gudiña, Eduardo J.; Rodrigues, Lígia R.; Fernandes, Fabiano A. N.; Rodrigues, Sueli, Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate. Biochemical Engineering Journal, 168(107954), 20211369-703X10.1016/j.bej.2021.107954http://www.elsevier.com/locate/issn/1369703Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:25:03Zoai:repositorium.sdum.uminho.pt:1822/70362Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:25:03Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate
title Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate
spellingShingle Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate
Meneses, D.
esterase
Aureobasidium pullulans URM 7059
airlift reactor
biodegradation
Science & Technology
title_short Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate
title_full Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate
title_fullStr Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate
title_full_unstemmed Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate
title_sort Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate
author Meneses, D.
author_facet Meneses, D.
Paixão, L.
Fonteles, Thatyane V.
Gudiña, Eduardo José
Rodrigues, L. R.
Fernandes, Fabiano A. N.
Rodrigues, Sueli
author_role author
author2 Paixão, L.
Fonteles, Thatyane V.
Gudiña, Eduardo José
Rodrigues, L. R.
Fernandes, Fabiano A. N.
Rodrigues, Sueli
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Meneses, D.
Paixão, L.
Fonteles, Thatyane V.
Gudiña, Eduardo José
Rodrigues, L. R.
Fernandes, Fabiano A. N.
Rodrigues, Sueli
dc.subject.por.fl_str_mv esterase
Aureobasidium pullulans URM 7059
airlift reactor
biodegradation
Science & Technology
topic esterase
Aureobasidium pullulans URM 7059
airlift reactor
biodegradation
Science & Technology
description Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.bej.2021.107954.
publishDate 2021
dc.date.none.fl_str_mv 2021-04
2021-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/70362
url http://hdl.handle.net/1822/70362
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Meneses, D.; Paixão, L.; Fonteles, Thatyane V.; Gudiña, Eduardo J.; Rodrigues, Lígia R.; Fernandes, Fabiano A. N.; Rodrigues, Sueli, Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate. Biochemical Engineering Journal, 168(107954), 2021
1369-703X
10.1016/j.bej.2021.107954
http://www.elsevier.com/locate/issn/1369703X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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