Integrated strategy for purification of esterase from Aureobasidium pullulans
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/55597 |
Resumo: | Supplementary data associated with this article can be found, in the online version, at https://doi.org/10.1016/j.seppur.2018.07.062. |
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Integrated strategy for purification of esterase from Aureobasidium pullulansEsteraseOlive mill wastewaterAqueous two-phase systemIntegrated strategyAureobasidium pullulansScience & TechnologySupplementary data associated with this article can be found, in the online version, at https://doi.org/10.1016/j.seppur.2018.07.062.Esterases catalyze the cleavage and formation of ester bonds of a broad range of substrates presenting a widespread spectrum of industrial applications. This work aimed to partially purify and characterize an esterase from Aureobasidium pullulans LABIOTEC 01 produced in a culture medium containing olive mill wastewater. Esterase purification was evaluated using different strategies, namely the enzyme recovery by PEG-salt aqueous two-phase systems (ATPSs); and the enzyme precipitation with ammonium sulfate, acetone, and ethanol. The best purification factor (18±2) was obtained when the ATPS composed of 20% (w/w) polyethylene glycol (PEG) 6000 and 5.8% (w/w) potassium phosphate buffer (PPB) pH 8.0 was combined with acetone precipitation. The partially purified enzyme presented an optimum pH of 5.0, although it remained active in the pH range of 4.5 to 7.5 ( 50% relative activity). The optimum temperature was found to be 60°C. Furthermore, the addition of salts such as FeCl3, CuSO4 and MnCl2 promoted an increase in the enzymatic activity (above 100%). The enzyme was found to be stable and showed high activity when exposed to polar solvents such as dimethyl sulfoxide, dimethylformamide, and methanol. The use of an integrated strategy of purification combining simple purification methods such as ATPS and precipitation was herein reported for the first time for esterase. This strategy proved to be an interesting approach to partially purify the esterase produced under submerged fermentation by A. pullulans. Furthermore, the enzyme showed potential to be applied in industrial biocatalytic processes using high temperature and different salts or solvents. Also, the production of esterase using olive mill wastewater as substrate demonstrated to be a suitable alternative to reduce and valorize agro-industrial residues.ACL acknowledges his post-doctoral grant and research funds from CAPES and CNPq (Brazilian funding agencies). SCS and LRR acknowledge their grants SFRH/BPD/88584/2012 and SFRH/BSAB/142873/ 2018, respectively, from the Portuguese Foundation for Science and Technology (FCT). The study also received financial support from FCT under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), the Project MultiBiorefinery - Multi‐purpose strategies for broadband agro‐forest and fisheries by‐products valorisation: a step forward for a truly integrated biorefinery (POCI-01-0145-FEDER-016403) and the Project Lignozymes - Metagenomics approach to unravel the potential of lignocellulosic residues towards the discovery of novel enzymes (POCI-010145-FEDER-029773). The authors also acknowledge financial support from the BioTecNorte operation (NORTE-01-0145-FEDER-000004) fundedbytheEuropeanRegionalDevelopment Fundunderthescopeof Norte2020 - Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoLemes, A.Silvério, Sara C.Rodrigues, SueliRodrigues, L. R.2019-01-312019-01-31T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/55597engLemes, A.; Silvério, Sara C.; Rodrigues, Sueli; Rodrigues, Lígia R., Integrated strategy for purification of esterase from Aureobasidium pullulans. Separation and Purification Technology, 209, 409-418, 20191383-58661383-586610.1016/j.seppur.2018.07.062https://www.journals.elsevier.com/separation-and-purification-technologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:15:59Zoai:repositorium.sdum.uminho.pt:1822/55597Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:08:32.281577Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Integrated strategy for purification of esterase from Aureobasidium pullulans |
| title |
Integrated strategy for purification of esterase from Aureobasidium pullulans |
| spellingShingle |
Integrated strategy for purification of esterase from Aureobasidium pullulans Lemes, A. Esterase Olive mill wastewater Aqueous two-phase system Integrated strategy Aureobasidium pullulans Science & Technology |
| title_short |
Integrated strategy for purification of esterase from Aureobasidium pullulans |
| title_full |
Integrated strategy for purification of esterase from Aureobasidium pullulans |
| title_fullStr |
Integrated strategy for purification of esterase from Aureobasidium pullulans |
| title_full_unstemmed |
Integrated strategy for purification of esterase from Aureobasidium pullulans |
| title_sort |
Integrated strategy for purification of esterase from Aureobasidium pullulans |
| author |
Lemes, A. |
| author_facet |
Lemes, A. Silvério, Sara C. Rodrigues, Sueli Rodrigues, L. R. |
| author_role |
author |
| author2 |
Silvério, Sara C. Rodrigues, Sueli Rodrigues, L. R. |
| author2_role |
author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Lemes, A. Silvério, Sara C. Rodrigues, Sueli Rodrigues, L. R. |
| dc.subject.por.fl_str_mv |
Esterase Olive mill wastewater Aqueous two-phase system Integrated strategy Aureobasidium pullulans Science & Technology |
| topic |
Esterase Olive mill wastewater Aqueous two-phase system Integrated strategy Aureobasidium pullulans Science & Technology |
| description |
Supplementary data associated with this article can be found, in the online version, at https://doi.org/10.1016/j.seppur.2018.07.062. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-01-31 2019-01-31T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/1822/55597 |
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http://hdl.handle.net/1822/55597 |
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eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Lemes, A.; Silvério, Sara C.; Rodrigues, Sueli; Rodrigues, Lígia R., Integrated strategy for purification of esterase from Aureobasidium pullulans. Separation and Purification Technology, 209, 409-418, 2019 1383-5866 1383-5866 10.1016/j.seppur.2018.07.062 https://www.journals.elsevier.com/separation-and-purification-technology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Elsevier |
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Elsevier |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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