Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/28868 |
Resumo: | Aspergillus carbonarius (Bainier) Thom. is an important pathogen and ochratoxin A (OTA) producer in grapes that can be controlled by adopting sustainable approaches. Here we evaluate the application of natural plant extracts as an alternative to synthetic fungicides to reduce OTA contamination and to prevent infection of grapes by two isolates of A. carbonarius. In a preliminary screening, natural extracts of chestnut flower, cistus, eucalyptus, fennel, and orange peel were evaluated for their antifungal and anti-mycotoxigenic efficiency in a grape-based medium at concentrations of 10 and 20 mg/mL. Cistus and orange peel extracts demonstrated the best anti- fungal activity at both concentrations. Although the eucalyptus extract demonstrated no significant effect on Aspergillus vegetative growth, it significantly reduced OTA by up to 85.75 % at 10 mg/mL compared to the control. Chestnut flower, cistus, eucalyptus, and orange peel extracts were then tested at the lowest concen- tration (10 mg/mL) for their antifungal activity in artificially inoculated grape berries. The cistus and orange peel extracts demonstrated the greatest antifungal activity and significantly reduced mold symptoms in grapes. Moreover, all tested natural extracts were able to reduce OTA content in grape berries (17.7 ± 8.3 % - 82.3 ± 3.85 % inhibition), although not always significantly. Eucalyptus extract was particularly efficient, inhibiting OTA production by both strains of A. carbonarius by up to >80 % with no effects on fungal growth. The use of natural eucalyptus extract represents a feasible strategy to reduce OTA formation without disrupting fungal growth, apparently maintaining the natural microbial balance, while cistus and orange peel extracts appear promising as inhibitors of A. carbonarius mycelial growth. Our findings suggest that plant extracts may be useful sources of bioactive chemicals for preventing A. carbonarius contamination and OTA production. Nonetheless, it will be necessary to evaluate their effect on the organoleptic properties of the grapes. |
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Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapesOTAMycotoxinsBiofungicidesFood safetyBioactivityAspergillus carbonarius (Bainier) Thom. is an important pathogen and ochratoxin A (OTA) producer in grapes that can be controlled by adopting sustainable approaches. Here we evaluate the application of natural plant extracts as an alternative to synthetic fungicides to reduce OTA contamination and to prevent infection of grapes by two isolates of A. carbonarius. In a preliminary screening, natural extracts of chestnut flower, cistus, eucalyptus, fennel, and orange peel were evaluated for their antifungal and anti-mycotoxigenic efficiency in a grape-based medium at concentrations of 10 and 20 mg/mL. Cistus and orange peel extracts demonstrated the best anti- fungal activity at both concentrations. Although the eucalyptus extract demonstrated no significant effect on Aspergillus vegetative growth, it significantly reduced OTA by up to 85.75 % at 10 mg/mL compared to the control. Chestnut flower, cistus, eucalyptus, and orange peel extracts were then tested at the lowest concen- tration (10 mg/mL) for their antifungal activity in artificially inoculated grape berries. The cistus and orange peel extracts demonstrated the greatest antifungal activity and significantly reduced mold symptoms in grapes. Moreover, all tested natural extracts were able to reduce OTA content in grape berries (17.7 ± 8.3 % - 82.3 ± 3.85 % inhibition), although not always significantly. Eucalyptus extract was particularly efficient, inhibiting OTA production by both strains of A. carbonarius by up to >80 % with no effects on fungal growth. The use of natural eucalyptus extract represents a feasible strategy to reduce OTA formation without disrupting fungal growth, apparently maintaining the natural microbial balance, while cistus and orange peel extracts appear promising as inhibitors of A. carbonarius mycelial growth. Our findings suggest that plant extracts may be useful sources of bioactive chemicals for preventing A. carbonarius contamination and OTA production. Nonetheless, it will be necessary to evaluate their effect on the organoleptic properties of the grapes.This work was supported by the Foundation for Science and Tech- nology (FCT, Portugal) #1 under Grant from national funds FCT/MCTES (PIDDAC) to CIMO [number UIDB/00690/2020 and UIDP/00690/ 2020] and SusTEC [number LA/P/0007/2020]; the European Regional Development Fund (ERDF) through the Competitiveness and Interna- tionalization Operational Program (CIOP) #2 under Grant dedicated to the project “PreVineGrape - Development of a biofungicide to combat grapevine diseases [number POCI-01-0247-FEDER-049695].ElsevierBiblioteca Digital do IPBChtioui, WiemHeleno, Sandrina A.Migheli, QuiricoRodrigues, Paula2023-11-06T11:02:04Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/28868engChtioui, Wiem; Heleno, Sandrina A.; Migheli, Quirico; Rodrigues, Paula (2023). Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes. International Journal of Food Microbiology. ISSN 0168-1605. 407, p. 1-110168-160510.1016/j.ijfoodmicro.2023.110425info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-21T01:18:39Zoai:bibliotecadigital.ipb.pt:10198/28868Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:18:48.234639Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes |
title |
Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes |
spellingShingle |
Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes Chtioui, Wiem OTA Mycotoxins Biofungicides Food safety Bioactivity |
title_short |
Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes |
title_full |
Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes |
title_fullStr |
Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes |
title_full_unstemmed |
Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes |
title_sort |
Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes |
author |
Chtioui, Wiem |
author_facet |
Chtioui, Wiem Heleno, Sandrina A. Migheli, Quirico Rodrigues, Paula |
author_role |
author |
author2 |
Heleno, Sandrina A. Migheli, Quirico Rodrigues, Paula |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Chtioui, Wiem Heleno, Sandrina A. Migheli, Quirico Rodrigues, Paula |
dc.subject.por.fl_str_mv |
OTA Mycotoxins Biofungicides Food safety Bioactivity |
topic |
OTA Mycotoxins Biofungicides Food safety Bioactivity |
description |
Aspergillus carbonarius (Bainier) Thom. is an important pathogen and ochratoxin A (OTA) producer in grapes that can be controlled by adopting sustainable approaches. Here we evaluate the application of natural plant extracts as an alternative to synthetic fungicides to reduce OTA contamination and to prevent infection of grapes by two isolates of A. carbonarius. In a preliminary screening, natural extracts of chestnut flower, cistus, eucalyptus, fennel, and orange peel were evaluated for their antifungal and anti-mycotoxigenic efficiency in a grape-based medium at concentrations of 10 and 20 mg/mL. Cistus and orange peel extracts demonstrated the best anti- fungal activity at both concentrations. Although the eucalyptus extract demonstrated no significant effect on Aspergillus vegetative growth, it significantly reduced OTA by up to 85.75 % at 10 mg/mL compared to the control. Chestnut flower, cistus, eucalyptus, and orange peel extracts were then tested at the lowest concen- tration (10 mg/mL) for their antifungal activity in artificially inoculated grape berries. The cistus and orange peel extracts demonstrated the greatest antifungal activity and significantly reduced mold symptoms in grapes. Moreover, all tested natural extracts were able to reduce OTA content in grape berries (17.7 ± 8.3 % - 82.3 ± 3.85 % inhibition), although not always significantly. Eucalyptus extract was particularly efficient, inhibiting OTA production by both strains of A. carbonarius by up to >80 % with no effects on fungal growth. The use of natural eucalyptus extract represents a feasible strategy to reduce OTA formation without disrupting fungal growth, apparently maintaining the natural microbial balance, while cistus and orange peel extracts appear promising as inhibitors of A. carbonarius mycelial growth. Our findings suggest that plant extracts may be useful sources of bioactive chemicals for preventing A. carbonarius contamination and OTA production. Nonetheless, it will be necessary to evaluate their effect on the organoleptic properties of the grapes. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-06T11:02:04Z 2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/28868 |
url |
http://hdl.handle.net/10198/28868 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chtioui, Wiem; Heleno, Sandrina A.; Migheli, Quirico; Rodrigues, Paula (2023). Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes. International Journal of Food Microbiology. ISSN 0168-1605. 407, p. 1-11 0168-1605 10.1016/j.ijfoodmicro.2023.110425 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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