An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5045 https://doi.org/10.1016/j.aca.2007.03.047 |
Resumo: | Improved biosensors for acetaldehyde determination have been developed using a bienzymatic strategy, based on a mediator-modified carbon film electrode and co-immobilisation of NADH oxidase and aldehyde dehydrogenase. Modification of the carbon film electrode with poly(neutral red) mediator resulted in a sensitive, low-cost and reliable NADH detector. Immobilisation of the enzymes was performed using encapsulation in a sol-gel matrix or cross-linking with glutaraldehyde. The bienzymatic biosensors were characterized by studying the influence of pH, applied potential and co-factors. The sol-gel and glutaraldehyde biosensors showed a linear response up to 60 [mu]M and 100 [mu]M, respectively, with detection limits of 2.6 [mu]M and 3.3 [mu]M and sensitivities were 1.7 [mu]A mM-1 and 5.6 [mu]A mM-1. The optimised biosensors showed good stability and good selectivity and have been tested for application for the determination of acetaldehyde in natural samples such as wine. |
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An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodesNicotinamide adenine dinucleotide oxidaseAldehyde dehydrogenaseCarbon film electrodePoly(neutral red)Enzyme cross-linkingSol-gelImproved biosensors for acetaldehyde determination have been developed using a bienzymatic strategy, based on a mediator-modified carbon film electrode and co-immobilisation of NADH oxidase and aldehyde dehydrogenase. Modification of the carbon film electrode with poly(neutral red) mediator resulted in a sensitive, low-cost and reliable NADH detector. Immobilisation of the enzymes was performed using encapsulation in a sol-gel matrix or cross-linking with glutaraldehyde. The bienzymatic biosensors were characterized by studying the influence of pH, applied potential and co-factors. The sol-gel and glutaraldehyde biosensors showed a linear response up to 60 [mu]M and 100 [mu]M, respectively, with detection limits of 2.6 [mu]M and 3.3 [mu]M and sensitivities were 1.7 [mu]A mM-1 and 5.6 [mu]A mM-1. The optimised biosensors showed good stability and good selectivity and have been tested for application for the determination of acetaldehyde in natural samples such as wine.http://www.sciencedirect.com/science/article/B6TF4-4NBH223-4/1/df3a7ae65fbb5f9739654441b2c733ed2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5045http://hdl.handle.net/10316/5045https://doi.org/10.1016/j.aca.2007.03.047engAnalytica Chimica Acta. 591:1 (2007) 80-86Ghica, Mariana EmiliaPauliukaite, RasaMarchand, NicolasDevic, EricBrett, Christopher M. A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-07-27T09:48:47Zoai:estudogeral.uc.pt:10316/5045Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:16.411192Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes |
title |
An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes |
spellingShingle |
An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes Ghica, Mariana Emilia Nicotinamide adenine dinucleotide oxidase Aldehyde dehydrogenase Carbon film electrode Poly(neutral red) Enzyme cross-linking Sol-gel |
title_short |
An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes |
title_full |
An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes |
title_fullStr |
An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes |
title_full_unstemmed |
An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes |
title_sort |
An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes |
author |
Ghica, Mariana Emilia |
author_facet |
Ghica, Mariana Emilia Pauliukaite, Rasa Marchand, Nicolas Devic, Eric Brett, Christopher M. A. |
author_role |
author |
author2 |
Pauliukaite, Rasa Marchand, Nicolas Devic, Eric Brett, Christopher M. A. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ghica, Mariana Emilia Pauliukaite, Rasa Marchand, Nicolas Devic, Eric Brett, Christopher M. A. |
dc.subject.por.fl_str_mv |
Nicotinamide adenine dinucleotide oxidase Aldehyde dehydrogenase Carbon film electrode Poly(neutral red) Enzyme cross-linking Sol-gel |
topic |
Nicotinamide adenine dinucleotide oxidase Aldehyde dehydrogenase Carbon film electrode Poly(neutral red) Enzyme cross-linking Sol-gel |
description |
Improved biosensors for acetaldehyde determination have been developed using a bienzymatic strategy, based on a mediator-modified carbon film electrode and co-immobilisation of NADH oxidase and aldehyde dehydrogenase. Modification of the carbon film electrode with poly(neutral red) mediator resulted in a sensitive, low-cost and reliable NADH detector. Immobilisation of the enzymes was performed using encapsulation in a sol-gel matrix or cross-linking with glutaraldehyde. The bienzymatic biosensors were characterized by studying the influence of pH, applied potential and co-factors. The sol-gel and glutaraldehyde biosensors showed a linear response up to 60 [mu]M and 100 [mu]M, respectively, with detection limits of 2.6 [mu]M and 3.3 [mu]M and sensitivities were 1.7 [mu]A mM-1 and 5.6 [mu]A mM-1. The optimised biosensors showed good stability and good selectivity and have been tested for application for the determination of acetaldehyde in natural samples such as wine. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5045 http://hdl.handle.net/10316/5045 https://doi.org/10.1016/j.aca.2007.03.047 |
url |
http://hdl.handle.net/10316/5045 https://doi.org/10.1016/j.aca.2007.03.047 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Analytica Chimica Acta. 591:1 (2007) 80-86 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133904830988288 |