Valorization of brewery waste through Polyhydroxyalkanoates production supported by a metabolic specialized microbiome

Detalhes bibliográficos
Autor(a) principal: Carvalheira, Mónica
Data de Publicação: 2022
Outros Autores: Amorim, Catarina L., Oliveira, Ana Catarina, Guarda, Eliana C., Costa, Eunice, Ribau Teixeira, Margarida, Castro, Paula M. L., Duque, Anouk F., Reis, Maria A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/18285
Resumo: Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the Meganema, Carnobacterium, Leucobacter, and Paracocccus genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 CmmolPHA/CmmolVFA in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products.
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spelling Valorization of brewery waste through Polyhydroxyalkanoates production supported by a metabolic specialized microbiomeBiopolymersCircular economyWaste valorizationMicrobial communityRaw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the Meganema, Carnobacterium, Leucobacter, and Paracocccus genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 CmmolPHA/CmmolVFA in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products.MDPISapientiaCarvalheira, MónicaAmorim, Catarina L.Oliveira, Ana CatarinaGuarda, Eliana C.Costa, EuniceRibau Teixeira, MargaridaCastro, Paula M. L.Duque, Anouk F.Reis, Maria A. M.2022-09-23T08:53:25Z2022-08-302022-09-22T12:02:21Z2022-08-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/18285eng10.3390/life12091347info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-29T10:41:52Zoai:sapientia.ualg.pt:10400.1/18285Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-29T10:41:52Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Valorization of brewery waste through Polyhydroxyalkanoates production supported by a metabolic specialized microbiome
title Valorization of brewery waste through Polyhydroxyalkanoates production supported by a metabolic specialized microbiome
spellingShingle Valorization of brewery waste through Polyhydroxyalkanoates production supported by a metabolic specialized microbiome
Carvalheira, Mónica
Biopolymers
Circular economy
Waste valorization
Microbial community
title_short Valorization of brewery waste through Polyhydroxyalkanoates production supported by a metabolic specialized microbiome
title_full Valorization of brewery waste through Polyhydroxyalkanoates production supported by a metabolic specialized microbiome
title_fullStr Valorization of brewery waste through Polyhydroxyalkanoates production supported by a metabolic specialized microbiome
title_full_unstemmed Valorization of brewery waste through Polyhydroxyalkanoates production supported by a metabolic specialized microbiome
title_sort Valorization of brewery waste through Polyhydroxyalkanoates production supported by a metabolic specialized microbiome
author Carvalheira, Mónica
author_facet Carvalheira, Mónica
Amorim, Catarina L.
Oliveira, Ana Catarina
Guarda, Eliana C.
Costa, Eunice
Ribau Teixeira, Margarida
Castro, Paula M. L.
Duque, Anouk F.
Reis, Maria A. M.
author_role author
author2 Amorim, Catarina L.
Oliveira, Ana Catarina
Guarda, Eliana C.
Costa, Eunice
Ribau Teixeira, Margarida
Castro, Paula M. L.
Duque, Anouk F.
Reis, Maria A. M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Carvalheira, Mónica
Amorim, Catarina L.
Oliveira, Ana Catarina
Guarda, Eliana C.
Costa, Eunice
Ribau Teixeira, Margarida
Castro, Paula M. L.
Duque, Anouk F.
Reis, Maria A. M.
dc.subject.por.fl_str_mv Biopolymers
Circular economy
Waste valorization
Microbial community
topic Biopolymers
Circular economy
Waste valorization
Microbial community
description Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the Meganema, Carnobacterium, Leucobacter, and Paracocccus genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 CmmolPHA/CmmolVFA in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-23T08:53:25Z
2022-08-30
2022-09-22T12:02:21Z
2022-08-30T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/18285
url http://hdl.handle.net/10400.1/18285
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.3390/life12091347
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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