Valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiome
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/38711 |
Resumo: | Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the Meganema, Carnobacterium, Leucobacter, and Paracocccus genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 CmmolPHA/CmmolVFA in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
Valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiomeBiopolymersCircular economyWaste valorizationMicrobial communityRaw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the Meganema, Carnobacterium, Leucobacter, and Paracocccus genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 CmmolPHA/CmmolVFA in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products.Veritati - Repositório Institucional da Universidade Católica PortuguesaCarvalheira, MónicaAmorim, Catarina L.Oliveira, Ana CatarinaGuarda, Eliana C.Costa, EuniceTeixeira, Margarida RibauCastro, Paula M. L.Duque, Anouk F.Reis, Maria A. M.2022-09-05T15:01:19Z2022-08-302022-08-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/38711eng10.3390/life120913472075-172985138720514PMC950589236143384000856706300001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T12:38:53Zoai:repositorio.ucp.pt:10400.14/38711Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T12:38:53Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiome |
title |
Valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiome |
spellingShingle |
Valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiome Carvalheira, Mónica Biopolymers Circular economy Waste valorization Microbial community |
title_short |
Valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiome |
title_full |
Valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiome |
title_fullStr |
Valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiome |
title_full_unstemmed |
Valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiome |
title_sort |
Valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiome |
author |
Carvalheira, Mónica |
author_facet |
Carvalheira, Mónica Amorim, Catarina L. Oliveira, Ana Catarina Guarda, Eliana C. Costa, Eunice Teixeira, Margarida Ribau Castro, Paula M. L. Duque, Anouk F. Reis, Maria A. M. |
author_role |
author |
author2 |
Amorim, Catarina L. Oliveira, Ana Catarina Guarda, Eliana C. Costa, Eunice Teixeira, Margarida Ribau Castro, Paula M. L. Duque, Anouk F. Reis, Maria A. M. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Carvalheira, Mónica Amorim, Catarina L. Oliveira, Ana Catarina Guarda, Eliana C. Costa, Eunice Teixeira, Margarida Ribau Castro, Paula M. L. Duque, Anouk F. Reis, Maria A. M. |
dc.subject.por.fl_str_mv |
Biopolymers Circular economy Waste valorization Microbial community |
topic |
Biopolymers Circular economy Waste valorization Microbial community |
description |
Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the Meganema, Carnobacterium, Leucobacter, and Paracocccus genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 CmmolPHA/CmmolVFA in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-05T15:01:19Z 2022-08-30 2022-08-30T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/38711 |
url |
http://hdl.handle.net/10400.14/38711 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.3390/life12091347 2075-1729 85138720514 PMC9505892 36143384 000856706300001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817547060213186560 |