Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing

Detalhes bibliográficos
Autor(a) principal: Ponte, Susana
Data de Publicação: 2020
Outros Autores: Carvalho, Lara, Gagliardi, Maria, Campos, Isabel, Oliveira, Paulo J., Jacinto, António
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/105814
https://doi.org/10.1242/bio.048629
Resumo: Mitochondria adapt to cellular needs by changes in morphology through fusion and fission events, referred to as mitochondrial dynamics. Mitochondrial function and morphology are intimately connected and the dysregulation of mitochondrial dynamics is linked to several human diseases. In this work, we investigated the role of mitochondrial dynamics in wound healing in the Drosophila embryonic epidermis. Mutants for mitochondrial fusion and fission proteins fail to close their wounds, indicating that the regulation of mitochondrial dynamics is required for wound healing. By live-imaging, we found that loss of function of the mitochondrial fission protein Dynamin-related protein 1 (Drp1) compromises the increase of cytosolic and mitochondrial calcium upon wounding and leads to reduced reactive oxygen species (ROS) production and F-actin defects at the wound edge, culminating in wound healing impairment. Our results highlight a new role for mitochondrial dynamics in the regulation of calcium, ROS and F-actin during epithelial repair.
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spelling Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healingCalcium; Drp1F-actinMitochondriaMitochondrial dynamicsWound healingActinsAnimalsBiomarkersCalciumCytoskeletal ProteinsDrosophilaGTP-Binding ProteinsImmunohistochemistryMitochondriaMitochondrial DynamicsMitochondrial ProteinsMutationReactive Oxygen SpeciesWound HealingMitochondria adapt to cellular needs by changes in morphology through fusion and fission events, referred to as mitochondrial dynamics. Mitochondrial function and morphology are intimately connected and the dysregulation of mitochondrial dynamics is linked to several human diseases. In this work, we investigated the role of mitochondrial dynamics in wound healing in the Drosophila embryonic epidermis. Mutants for mitochondrial fusion and fission proteins fail to close their wounds, indicating that the regulation of mitochondrial dynamics is required for wound healing. By live-imaging, we found that loss of function of the mitochondrial fission protein Dynamin-related protein 1 (Drp1) compromises the increase of cytosolic and mitochondrial calcium upon wounding and leads to reduced reactive oxygen species (ROS) production and F-actin defects at the wound edge, culminating in wound healing impairment. Our results highlight a new role for mitochondrial dynamics in the regulation of calcium, ROS and F-actin during epithelial repair.The Company of Biologists Ltd.2020-05-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/105814http://hdl.handle.net/10316/105814https://doi.org/10.1242/bio.048629eng2046-6390Ponte, SusanaCarvalho, LaraGagliardi, MariaCampos, IsabelOliveira, Paulo J.Jacinto, Antónioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-09T21:31:07Zoai:estudogeral.uc.pt:10316/105814Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:22:18.846328Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
title Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
spellingShingle Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
Ponte, Susana
Calcium; Drp1
F-actin
Mitochondria
Mitochondrial dynamics
Wound healing
Actins
Animals
Biomarkers
Calcium
Cytoskeletal Proteins
Drosophila
GTP-Binding Proteins
Immunohistochemistry
Mitochondria
Mitochondrial Dynamics
Mitochondrial Proteins
Mutation
Reactive Oxygen Species
Wound Healing
title_short Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
title_full Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
title_fullStr Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
title_full_unstemmed Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
title_sort Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
author Ponte, Susana
author_facet Ponte, Susana
Carvalho, Lara
Gagliardi, Maria
Campos, Isabel
Oliveira, Paulo J.
Jacinto, António
author_role author
author2 Carvalho, Lara
Gagliardi, Maria
Campos, Isabel
Oliveira, Paulo J.
Jacinto, António
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ponte, Susana
Carvalho, Lara
Gagliardi, Maria
Campos, Isabel
Oliveira, Paulo J.
Jacinto, António
dc.subject.por.fl_str_mv Calcium; Drp1
F-actin
Mitochondria
Mitochondrial dynamics
Wound healing
Actins
Animals
Biomarkers
Calcium
Cytoskeletal Proteins
Drosophila
GTP-Binding Proteins
Immunohistochemistry
Mitochondria
Mitochondrial Dynamics
Mitochondrial Proteins
Mutation
Reactive Oxygen Species
Wound Healing
topic Calcium; Drp1
F-actin
Mitochondria
Mitochondrial dynamics
Wound healing
Actins
Animals
Biomarkers
Calcium
Cytoskeletal Proteins
Drosophila
GTP-Binding Proteins
Immunohistochemistry
Mitochondria
Mitochondrial Dynamics
Mitochondrial Proteins
Mutation
Reactive Oxygen Species
Wound Healing
description Mitochondria adapt to cellular needs by changes in morphology through fusion and fission events, referred to as mitochondrial dynamics. Mitochondrial function and morphology are intimately connected and the dysregulation of mitochondrial dynamics is linked to several human diseases. In this work, we investigated the role of mitochondrial dynamics in wound healing in the Drosophila embryonic epidermis. Mutants for mitochondrial fusion and fission proteins fail to close their wounds, indicating that the regulation of mitochondrial dynamics is required for wound healing. By live-imaging, we found that loss of function of the mitochondrial fission protein Dynamin-related protein 1 (Drp1) compromises the increase of cytosolic and mitochondrial calcium upon wounding and leads to reduced reactive oxygen species (ROS) production and F-actin defects at the wound edge, culminating in wound healing impairment. Our results highlight a new role for mitochondrial dynamics in the regulation of calcium, ROS and F-actin during epithelial repair.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-03
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/105814
http://hdl.handle.net/10316/105814
https://doi.org/10.1242/bio.048629
url http://hdl.handle.net/10316/105814
https://doi.org/10.1242/bio.048629
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2046-6390
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv The Company of Biologists Ltd.
publisher.none.fl_str_mv The Company of Biologists Ltd.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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