Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing

Detalhes bibliográficos
Autor(a) principal: Ponte, Susana
Data de Publicação: 2020
Outros Autores: Carvalho, Lara, Gagliardi, Maria, Campos, Isabel, Oliveira, Paulo J, Jacinto, António
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/96540
Resumo: This work was supported by national funds through FCT - Fundação para a Ciência e a Tecnologia, I.P., in the context of a program contract to L. Carvalho (4, 5 and 6 of article 23.º of D.L. no. 57/2016 of 29 August, as amended by Law no. 57/2017 of 19 July), PD/BD/106058/2015 to S. Ponte and PTDC/BIA-BID/29709/2017; the European Research Council (2007-StG-208631) and CONGENTO LISBOA-01-0145-FEDER-022170.
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spelling Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healingCalciumDrp1F-actinMitochondriaMitochondrial dynamicsWound healingSDG 3 - Good Health and Well-beingThis work was supported by national funds through FCT - Fundação para a Ciência e a Tecnologia, I.P., in the context of a program contract to L. Carvalho (4, 5 and 6 of article 23.º of D.L. no. 57/2016 of 29 August, as amended by Law no. 57/2017 of 19 July), PD/BD/106058/2015 to S. Ponte and PTDC/BIA-BID/29709/2017; the European Research Council (2007-StG-208631) and CONGENTO LISBOA-01-0145-FEDER-022170.Mitochondria adapt to cellular needs by changes in morphology through fusion and fission events, referred to as mitochondrial dynamics. Mitochondrial function and morphology are intimately connected and the dysregulation of mitochondrial dynamics is linked to several human diseases. In this work, we investigated the role of mitochondrial dynamics in wound healing in the Drosophila embryonic epidermis. Mutants for mitochondrial fusion and fission proteins fail to close their wounds, indicating that the regulation of mitochondrial dynamics is required for wound healing. By live-imaging, we found that loss of function of the mitochondrial fission protein Dynamin-related protein 1 (Drp1) compromises the increase of cytosolic and mitochondrial calcium upon wounding and leads to reduced ROS production and F-actin defects at the wound edge, culminating in wound healing impairment. Our results highlight a new role for mitochondrial dynamics in the regulation of calcium, ROS and F-actin during epithelial repair.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNPonte, SusanaCarvalho, LaraGagliardi, MariaCampos, IsabelOliveira, Paulo JJacinto, António2020-04-20T22:33:47Z2020-052020-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/96540eng2046-6390PURE: 17766651https://doi.org/10.1242/bio.048629info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:44:09Zoai:run.unl.pt:10362/96540Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:38:36.070017Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
title Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
spellingShingle Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
Ponte, Susana
Calcium
Drp1
F-actin
Mitochondria
Mitochondrial dynamics
Wound healing
SDG 3 - Good Health and Well-being
title_short Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
title_full Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
title_fullStr Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
title_full_unstemmed Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
title_sort Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing
author Ponte, Susana
author_facet Ponte, Susana
Carvalho, Lara
Gagliardi, Maria
Campos, Isabel
Oliveira, Paulo J
Jacinto, António
author_role author
author2 Carvalho, Lara
Gagliardi, Maria
Campos, Isabel
Oliveira, Paulo J
Jacinto, António
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Ponte, Susana
Carvalho, Lara
Gagliardi, Maria
Campos, Isabel
Oliveira, Paulo J
Jacinto, António
dc.subject.por.fl_str_mv Calcium
Drp1
F-actin
Mitochondria
Mitochondrial dynamics
Wound healing
SDG 3 - Good Health and Well-being
topic Calcium
Drp1
F-actin
Mitochondria
Mitochondrial dynamics
Wound healing
SDG 3 - Good Health and Well-being
description This work was supported by national funds through FCT - Fundação para a Ciência e a Tecnologia, I.P., in the context of a program contract to L. Carvalho (4, 5 and 6 of article 23.º of D.L. no. 57/2016 of 29 August, as amended by Law no. 57/2017 of 19 July), PD/BD/106058/2015 to S. Ponte and PTDC/BIA-BID/29709/2017; the European Research Council (2007-StG-208631) and CONGENTO LISBOA-01-0145-FEDER-022170.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-20T22:33:47Z
2020-05
2020-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/96540
url http://hdl.handle.net/10362/96540
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2046-6390
PURE: 17766651
https://doi.org/10.1242/bio.048629
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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