Bacteria and Protozoa Differentially Modulate the Expression of Rab Proteins

Detalhes bibliográficos
Autor(a) principal: Ramalho, José da Silva
Data de Publicação: 2012
Outros Autores: Mota, Lu­s Jaime, Barral, Duarte Custal Ferreira, Seabra, Miguel Pedro Pires Cardoso de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/51229
Resumo: e39858
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spelling Bacteria and Protozoa Differentially Modulate the Expression of Rab ProteinsPHAGOSOME MATURATIONTRAFFICKINGTRANSLOCATIONKISSARRESTGUTFUSIONGTPASESMALARIAe39858Phagocytic cells represent an important line of innate defense against microorganisms. Uptake of microorganisms by these cells involves the formation of a phagosome that matures by fusing with endocytic compartments, resulting in killing of the enclosed microbe. Small GTPases of the Rab family are key regulators of vesicular trafficking in the endocytic pathway. Intracellular pathogens can interfere with the function of these proteins in order to subvert host immune responses. However, it is unknown if this subversion can be achieved through the modulation of Rab gene expression. We compared the expression level of 23 distinct Rab GTPases in mouse macrophages after infection with the protozoan Plasmodium berghei, and the bacteria Escherichia coli and Salmonella enterica. We found that P. berghei induces an increase in the expression of a different set of Rab genes than E. coli and S. enterica, which behaved similarly. Strikingly, when one of the Rab proteins whose expression was increased by P. berghei, namely Rab14, was silenced, we observed a significant increase in the phagocytosis of P. berghei, whereas Rab14 overexpression led to a decrease in phagocytosis. This suggests that the parasite might induce the increase of Rab14 expression for its own advantage. Similarly, when Rab9a, whose expression was increased by E. coli and S. enterica, was silenced, we observed an increase in the phagocytosis of both bacterial species, whereas Rab9a overexpression caused a reduction in phagocytosis. This further suggests that the modulation of Rab gene expression could represent a mechanism of immune evasion. Thus, our study analyzes the modulation of Rab gene expression induced by bacteria and protozoa and suggests that this modulation could be necessary for the success of microbial infection.Centro de Estudos de Doenças Crónicas (CEDOC)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNRamalho, José da SilvaMota, Lu­s JaimeBarral, Duarte Custal FerreiraSeabra, Miguel Pedro Pires Cardoso de2018-11-09T23:06:50Z2012-07-202012-07-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/51229engPURE: 401711https://doi.org/10.1371/journal.pone.0039858info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:25:40Zoai:run.unl.pt:10362/51229Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:32:26.263341Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Bacteria and Protozoa Differentially Modulate the Expression of Rab Proteins
title Bacteria and Protozoa Differentially Modulate the Expression of Rab Proteins
spellingShingle Bacteria and Protozoa Differentially Modulate the Expression of Rab Proteins
Ramalho, José da Silva
PHAGOSOME MATURATION
TRAFFICKING
TRANSLOCATION
KISS
ARREST
GUT
FUSION
GTPASES
MALARIA
title_short Bacteria and Protozoa Differentially Modulate the Expression of Rab Proteins
title_full Bacteria and Protozoa Differentially Modulate the Expression of Rab Proteins
title_fullStr Bacteria and Protozoa Differentially Modulate the Expression of Rab Proteins
title_full_unstemmed Bacteria and Protozoa Differentially Modulate the Expression of Rab Proteins
title_sort Bacteria and Protozoa Differentially Modulate the Expression of Rab Proteins
author Ramalho, José da Silva
author_facet Ramalho, José da Silva
Mota, Lu­s Jaime
Barral, Duarte Custal Ferreira
Seabra, Miguel Pedro Pires Cardoso de
author_role author
author2 Mota, Lu­s Jaime
Barral, Duarte Custal Ferreira
Seabra, Miguel Pedro Pires Cardoso de
author2_role author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Ramalho, José da Silva
Mota, Lu­s Jaime
Barral, Duarte Custal Ferreira
Seabra, Miguel Pedro Pires Cardoso de
dc.subject.por.fl_str_mv PHAGOSOME MATURATION
TRAFFICKING
TRANSLOCATION
KISS
ARREST
GUT
FUSION
GTPASES
MALARIA
topic PHAGOSOME MATURATION
TRAFFICKING
TRANSLOCATION
KISS
ARREST
GUT
FUSION
GTPASES
MALARIA
description e39858
publishDate 2012
dc.date.none.fl_str_mv 2012-07-20
2012-07-20T00:00:00Z
2018-11-09T23:06:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/51229
url http://hdl.handle.net/10362/51229
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 401711
https://doi.org/10.1371/journal.pone.0039858
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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