Surface Complexation of DNA with Insoluble Monolayers. Influence of Divalent Counterions

Detalhes bibliográficos
Autor(a) principal: McLoughlin, D.
Data de Publicação: 2005
Outros Autores: Dias, R., Lindman, B., Cardenas, M., Nylander, T., Dawson, K., Miguel, M., Langevin, D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/10472
https://doi.org/10.1021/la047700s
Resumo: DNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease of surface potential, while an increase is observed for the DSPC monolayers. Binding to dipalmitoyl-sn-glycero-3-phosphocholine preserves most of the liquid expanded−liquid condensed coexistence region. The liquid condensed domains adopt an elongated morphology in the presence of DNA, especially in the presence of calcium. The interaction of DNA with phospholipid monolayers is ion specific: the presence of calcium leads to a stronger interaction than magnesium and barium. These results were confirmed by bulk complexation studies.
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spelling Surface Complexation of DNA with Insoluble Monolayers. Influence of Divalent CounterionsDNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease of surface potential, while an increase is observed for the DSPC monolayers. Binding to dipalmitoyl-sn-glycero-3-phosphocholine preserves most of the liquid expanded−liquid condensed coexistence region. The liquid condensed domains adopt an elongated morphology in the presence of DNA, especially in the presence of calcium. The interaction of DNA with phospholipid monolayers is ion specific: the presence of calcium leads to a stronger interaction than magnesium and barium. These results were confirmed by bulk complexation studies.American Chemical Society2005-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10472http://hdl.handle.net/10316/10472https://doi.org/10.1021/la047700sengLangmuir. 21:5 (2005) 1900-19070743-7463McLoughlin, D.Dias, R.Lindman, B.Cardenas, M.Nylander, T.Dawson, K.Miguel, M.Langevin, D.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-25T13:15:30Zoai:estudogeral.uc.pt:10316/10472Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:30.110803Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Surface Complexation of DNA with Insoluble Monolayers. Influence of Divalent Counterions
title Surface Complexation of DNA with Insoluble Monolayers. Influence of Divalent Counterions
spellingShingle Surface Complexation of DNA with Insoluble Monolayers. Influence of Divalent Counterions
McLoughlin, D.
title_short Surface Complexation of DNA with Insoluble Monolayers. Influence of Divalent Counterions
title_full Surface Complexation of DNA with Insoluble Monolayers. Influence of Divalent Counterions
title_fullStr Surface Complexation of DNA with Insoluble Monolayers. Influence of Divalent Counterions
title_full_unstemmed Surface Complexation of DNA with Insoluble Monolayers. Influence of Divalent Counterions
title_sort Surface Complexation of DNA with Insoluble Monolayers. Influence of Divalent Counterions
author McLoughlin, D.
author_facet McLoughlin, D.
Dias, R.
Lindman, B.
Cardenas, M.
Nylander, T.
Dawson, K.
Miguel, M.
Langevin, D.
author_role author
author2 Dias, R.
Lindman, B.
Cardenas, M.
Nylander, T.
Dawson, K.
Miguel, M.
Langevin, D.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv McLoughlin, D.
Dias, R.
Lindman, B.
Cardenas, M.
Nylander, T.
Dawson, K.
Miguel, M.
Langevin, D.
description DNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease of surface potential, while an increase is observed for the DSPC monolayers. Binding to dipalmitoyl-sn-glycero-3-phosphocholine preserves most of the liquid expanded−liquid condensed coexistence region. The liquid condensed domains adopt an elongated morphology in the presence of DNA, especially in the presence of calcium. The interaction of DNA with phospholipid monolayers is ion specific: the presence of calcium leads to a stronger interaction than magnesium and barium. These results were confirmed by bulk complexation studies.
publishDate 2005
dc.date.none.fl_str_mv 2005-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/10472
http://hdl.handle.net/10316/10472
https://doi.org/10.1021/la047700s
url http://hdl.handle.net/10316/10472
https://doi.org/10.1021/la047700s
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Langmuir. 21:5 (2005) 1900-1907
0743-7463
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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