Atomic force microscopy assisted with electron and ion beam microscopy for the direct measurement of biostructures and DNA samples

Detalhes bibliográficos
Autor(a) principal: Calmeiro, Tomás R.
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/10851
Resumo: Dissertation to obtain a Master degree in Biotechnology
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spelling Atomic force microscopy assisted with electron and ion beam microscopy for the direct measurement of biostructures and DNA samplesAtomic force microscopyAFMBionanotechnologyDNA-protein interactionAFM probe modificationsFocused ion beamDissertation to obtain a Master degree in BiotechnologyStudies of atomic force microscopy (AFM), a technique commonly associated with material sciences, in the field of biotechnology, with special emphasis on molecular biology and bionanotechnology elements, are presented herein. DNA, insulin and restriction enzymes were analysed as isolated objects, in order to document properties like morphology and behaviour, and also on the interactions between each other. The interaction between DNA and the EcoRV restriction endonuclease was especially highlighted. Gold nanoparticles were also studied in an attempt to establish a relationship between functionalization and average diameter shifts, as perceived by AFM. Given the nature of these objects of study, both immobilization and preparation protocols were modified and evaluated, considering measurements in air or in liquid. To serve the objective of developing probes with higher sensitivity and resolution, especially for liquid measurements, AFM cantilevers were modified through metallic coatings and scanning electron microscopy assisted with focused ion beam (SEM-FIB). Tests performed with these probes confirmed that the modifications were successful, the probes are functional and can aid in the production of better quality images, through the acquisition of sharper data in comparison with non-modified probes.Faculdade de Ciências e TecnologiaBusani, TitoTavares, JoséRUNCalmeiro, Tomás R.2013-12-11T17:27:41Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10851TID:201753804enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:14:40Zoai:run.unl.pt:10362/10851Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:14:40Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Atomic force microscopy assisted with electron and ion beam microscopy for the direct measurement of biostructures and DNA samples
title Atomic force microscopy assisted with electron and ion beam microscopy for the direct measurement of biostructures and DNA samples
spellingShingle Atomic force microscopy assisted with electron and ion beam microscopy for the direct measurement of biostructures and DNA samples
Calmeiro, Tomás R.
Atomic force microscopy
AFM
Bionanotechnology
DNA-protein interaction
AFM probe modifications
Focused ion beam
title_short Atomic force microscopy assisted with electron and ion beam microscopy for the direct measurement of biostructures and DNA samples
title_full Atomic force microscopy assisted with electron and ion beam microscopy for the direct measurement of biostructures and DNA samples
title_fullStr Atomic force microscopy assisted with electron and ion beam microscopy for the direct measurement of biostructures and DNA samples
title_full_unstemmed Atomic force microscopy assisted with electron and ion beam microscopy for the direct measurement of biostructures and DNA samples
title_sort Atomic force microscopy assisted with electron and ion beam microscopy for the direct measurement of biostructures and DNA samples
author Calmeiro, Tomás R.
author_facet Calmeiro, Tomás R.
author_role author
dc.contributor.none.fl_str_mv Busani, Tito
Tavares, José
RUN
dc.contributor.author.fl_str_mv Calmeiro, Tomás R.
dc.subject.por.fl_str_mv Atomic force microscopy
AFM
Bionanotechnology
DNA-protein interaction
AFM probe modifications
Focused ion beam
topic Atomic force microscopy
AFM
Bionanotechnology
DNA-protein interaction
AFM probe modifications
Focused ion beam
description Dissertation to obtain a Master degree in Biotechnology
publishDate 2013
dc.date.none.fl_str_mv 2013-12-11T17:27:41Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10851
TID:201753804
url http://hdl.handle.net/10362/10851
identifier_str_mv TID:201753804
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817545502032396288

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