Biosensors for the Polyphenolic Content of Wine Determination

Detalhes bibliográficos
Autor(a) principal: Júnior,A.R.S.
Data de Publicação: 2008
Outros Autores: Rebelo,M.J.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009
Resumo: Laccase of different lots was used in the construction of biosensors for the determination of polyphenolic content of wine. Laccase was immobilized on modified polyethersulfone membranes and applied to a Universal Sensor electrode base system. Parameters of the biosensors thus prepared were studied with respect to caffeic acid as the model substrate. Sensitivity of 0.102 mA × M-1, RSD of 1.9%, linear range of 5 to 35 × 10-6 M, limit of detection of 8.8 × 10-7M, were determined. Different values of the applied potential near 0 V were tested in order to choose that one which gives the best performance of the present biosensors. Citrate and tartarate buffer solutions at pH 3.5 were used. Polyphenolic content of different red wines was determined at +100 mV vs. Ag/AgCl.
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spelling Biosensors for the Polyphenolic Content of Wine Determinationpolyphenolicwinebiosensorlaccasecaffeic acidLaccase of different lots was used in the construction of biosensors for the determination of polyphenolic content of wine. Laccase was immobilized on modified polyethersulfone membranes and applied to a Universal Sensor electrode base system. Parameters of the biosensors thus prepared were studied with respect to caffeic acid as the model substrate. Sensitivity of 0.102 mA × M-1, RSD of 1.9%, linear range of 5 to 35 × 10-6 M, limit of detection of 8.8 × 10-7M, were determined. Different values of the applied potential near 0 V were tested in order to choose that one which gives the best performance of the present biosensors. Citrate and tartarate buffer solutions at pH 3.5 were used. Polyphenolic content of different red wines was determined at +100 mV vs. Ag/AgCl.Sociedade Portuguesa de Electroquímica2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009Portugaliae Electrochimica Acta v.26 n.1 2008reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009Júnior,A.R.S.Rebelo,M.J.F.info:eu-repo/semantics/openAccess2024-02-06T17:06:54Zoai:scielo:S0872-19042008000100009Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:20:03.825829Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biosensors for the Polyphenolic Content of Wine Determination
title Biosensors for the Polyphenolic Content of Wine Determination
spellingShingle Biosensors for the Polyphenolic Content of Wine Determination
Júnior,A.R.S.
polyphenolic
wine
biosensor
laccase
caffeic acid
title_short Biosensors for the Polyphenolic Content of Wine Determination
title_full Biosensors for the Polyphenolic Content of Wine Determination
title_fullStr Biosensors for the Polyphenolic Content of Wine Determination
title_full_unstemmed Biosensors for the Polyphenolic Content of Wine Determination
title_sort Biosensors for the Polyphenolic Content of Wine Determination
author Júnior,A.R.S.
author_facet Júnior,A.R.S.
Rebelo,M.J.F.
author_role author
author2 Rebelo,M.J.F.
author2_role author
dc.contributor.author.fl_str_mv Júnior,A.R.S.
Rebelo,M.J.F.
dc.subject.por.fl_str_mv polyphenolic
wine
biosensor
laccase
caffeic acid
topic polyphenolic
wine
biosensor
laccase
caffeic acid
description Laccase of different lots was used in the construction of biosensors for the determination of polyphenolic content of wine. Laccase was immobilized on modified polyethersulfone membranes and applied to a Universal Sensor electrode base system. Parameters of the biosensors thus prepared were studied with respect to caffeic acid as the model substrate. Sensitivity of 0.102 mA × M-1, RSD of 1.9%, linear range of 5 to 35 × 10-6 M, limit of detection of 8.8 × 10-7M, were determined. Different values of the applied potential near 0 V were tested in order to choose that one which gives the best performance of the present biosensors. Citrate and tartarate buffer solutions at pH 3.5 were used. Polyphenolic content of different red wines was determined at +100 mV vs. Ag/AgCl.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009
url http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Portuguesa de Electroquímica
publisher.none.fl_str_mv Sociedade Portuguesa de Electroquímica
dc.source.none.fl_str_mv Portugaliae Electrochimica Acta v.26 n.1 2008
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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