Biosensors for the Polyphenolic Content of Wine Determination
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009 |
Resumo: | Laccase of different lots was used in the construction of biosensors for the determination of polyphenolic content of wine. Laccase was immobilized on modified polyethersulfone membranes and applied to a Universal Sensor electrode base system. Parameters of the biosensors thus prepared were studied with respect to caffeic acid as the model substrate. Sensitivity of 0.102 mA × M-1, RSD of 1.9%, linear range of 5 to 35 × 10-6 M, limit of detection of 8.8 × 10-7M, were determined. Different values of the applied potential near 0 V were tested in order to choose that one which gives the best performance of the present biosensors. Citrate and tartarate buffer solutions at pH 3.5 were used. Polyphenolic content of different red wines was determined at +100 mV vs. Ag/AgCl. |
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Biosensors for the Polyphenolic Content of Wine Determinationpolyphenolicwinebiosensorlaccasecaffeic acidLaccase of different lots was used in the construction of biosensors for the determination of polyphenolic content of wine. Laccase was immobilized on modified polyethersulfone membranes and applied to a Universal Sensor electrode base system. Parameters of the biosensors thus prepared were studied with respect to caffeic acid as the model substrate. Sensitivity of 0.102 mA × M-1, RSD of 1.9%, linear range of 5 to 35 × 10-6 M, limit of detection of 8.8 × 10-7M, were determined. Different values of the applied potential near 0 V were tested in order to choose that one which gives the best performance of the present biosensors. Citrate and tartarate buffer solutions at pH 3.5 were used. Polyphenolic content of different red wines was determined at +100 mV vs. Ag/AgCl.Sociedade Portuguesa de Electroquímica2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009Portugaliae Electrochimica Acta v.26 n.1 2008reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009Júnior,A.R.S.Rebelo,M.J.F.info:eu-repo/semantics/openAccess2024-02-06T17:06:54Zoai:scielo:S0872-19042008000100009Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:20:03.825829Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biosensors for the Polyphenolic Content of Wine Determination |
title |
Biosensors for the Polyphenolic Content of Wine Determination |
spellingShingle |
Biosensors for the Polyphenolic Content of Wine Determination Júnior,A.R.S. polyphenolic wine biosensor laccase caffeic acid |
title_short |
Biosensors for the Polyphenolic Content of Wine Determination |
title_full |
Biosensors for the Polyphenolic Content of Wine Determination |
title_fullStr |
Biosensors for the Polyphenolic Content of Wine Determination |
title_full_unstemmed |
Biosensors for the Polyphenolic Content of Wine Determination |
title_sort |
Biosensors for the Polyphenolic Content of Wine Determination |
author |
Júnior,A.R.S. |
author_facet |
Júnior,A.R.S. Rebelo,M.J.F. |
author_role |
author |
author2 |
Rebelo,M.J.F. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Júnior,A.R.S. Rebelo,M.J.F. |
dc.subject.por.fl_str_mv |
polyphenolic wine biosensor laccase caffeic acid |
topic |
polyphenolic wine biosensor laccase caffeic acid |
description |
Laccase of different lots was used in the construction of biosensors for the determination of polyphenolic content of wine. Laccase was immobilized on modified polyethersulfone membranes and applied to a Universal Sensor electrode base system. Parameters of the biosensors thus prepared were studied with respect to caffeic acid as the model substrate. Sensitivity of 0.102 mA × M-1, RSD of 1.9%, linear range of 5 to 35 × 10-6 M, limit of detection of 8.8 × 10-7M, were determined. Different values of the applied potential near 0 V were tested in order to choose that one which gives the best performance of the present biosensors. Citrate and tartarate buffer solutions at pH 3.5 were used. Polyphenolic content of different red wines was determined at +100 mV vs. Ag/AgCl. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009 |
url |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Portuguesa de Electroquímica |
publisher.none.fl_str_mv |
Sociedade Portuguesa de Electroquímica |
dc.source.none.fl_str_mv |
Portugaliae Electrochimica Acta v.26 n.1 2008 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1817552293467258880 |