Development of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samples

Detalhes bibliográficos
Autor(a) principal: Filipa-Silva, Andreia
Data de Publicação: 2021
Outros Autores: Nunes, Mónica, Parreira, Ricardo, Crespo, Maria Teresa Barreto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/119238
Resumo: Funding Information: from Multi Biorefinery project (POCI-01?0145-FEDER-016403, INTERFACE Program, through the Innovation, Technology and Circular Economy Fund (FITEC) (CIT/2018/15) and FCT for Andreia Silva fellowship (PD/BD/128363/2017) are gratefully acknowledged. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
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spelling Development of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samplesFishHepatitis ANorovirusPathogenic human virusesTetraplex qPCR assayMicrobiologyMicrobiology (medical)VirologyMolecular BiologySDG 3 - Good Health and Well-beingSDG 12 - Responsible Consumption and ProductionSDG 14 - Life Below WaterFunding Information: from Multi Biorefinery project (POCI-01?0145-FEDER-016403, INTERFACE Program, through the Innovation, Technology and Circular Economy Fund (FITEC) (CIT/2018/15) and FCT for Andreia Silva fellowship (PD/BD/128363/2017) are gratefully acknowledged. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.Human enteric viruses such as norovirus (NoV) and hepatitis A virus (HAV) are some of the most important causes of foodborne infections worldwide. Usually, infection via fish consumption is not a concern regarding these viruses, since fish are mainly consumed cooked. However, in the last years, raw fish consumption has become increasingly common, especially involving the use of seabass and gilthead seabream in dishes like sushi, sashimi, poke, and carpaccio. Therefore, the risk for viral infection via the consumption of raw fish has also increased. In this study, a virologic screening was performed in 323 fish specimens captured along the Portuguese coast using a tetraplex qPCR optimised for two templates (plasmid and in vitro transcribed RNA) to detect and quantify NoV GI, NoV GII and HAV genomes. A difference of approximately 1-log was found between the use of plasmid or in vitro transcribed RNA for molecular-based quantifications, showing an underestimation of genome copy-number equivalents using plasmid standard-based curves. Additionally, the presence of NoV genomic RNA in a pool of seabass brains was identified, which was shown to cluster with a major group of human norovirus sequences from genogroup I (GI.1) by phylogenetic analysis. None of the analysed fish revealed the presence of NoV GII or HAV. This result corroborates the hypothesis that enteric viruses circulate in seawater or that fish were contaminated during their transportation/handling, representing a potential risk to humans through raw or undercooked fish consumption.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)RUNFilipa-Silva, AndreiaNunes, MónicaParreira, RicardoCrespo, Maria Teresa Barreto2021-06-14T22:16:18Z2021-062021-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/119238eng2076-2607PURE: 31821147https://doi.org/10.3390/microorganisms9061149info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:01:45Zoai:run.unl.pt:10362/119238Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:59.545533Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samples
title Development of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samples
spellingShingle Development of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samples
Filipa-Silva, Andreia
Fish
Hepatitis A
Norovirus
Pathogenic human viruses
Tetraplex qPCR assay
Microbiology
Microbiology (medical)
Virology
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 12 - Responsible Consumption and Production
SDG 14 - Life Below Water
title_short Development of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samples
title_full Development of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samples
title_fullStr Development of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samples
title_full_unstemmed Development of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samples
title_sort Development of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samples
author Filipa-Silva, Andreia
author_facet Filipa-Silva, Andreia
Nunes, Mónica
Parreira, Ricardo
Crespo, Maria Teresa Barreto
author_role author
author2 Nunes, Mónica
Parreira, Ricardo
Crespo, Maria Teresa Barreto
author2_role author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
Instituto de Higiene e Medicina Tropical (IHMT)
Global Health and Tropical Medicine (GHTM)
Vector borne diseases and pathogens (VBD)
RUN
dc.contributor.author.fl_str_mv Filipa-Silva, Andreia
Nunes, Mónica
Parreira, Ricardo
Crespo, Maria Teresa Barreto
dc.subject.por.fl_str_mv Fish
Hepatitis A
Norovirus
Pathogenic human viruses
Tetraplex qPCR assay
Microbiology
Microbiology (medical)
Virology
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 12 - Responsible Consumption and Production
SDG 14 - Life Below Water
topic Fish
Hepatitis A
Norovirus
Pathogenic human viruses
Tetraplex qPCR assay
Microbiology
Microbiology (medical)
Virology
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 12 - Responsible Consumption and Production
SDG 14 - Life Below Water
description Funding Information: from Multi Biorefinery project (POCI-01?0145-FEDER-016403, INTERFACE Program, through the Innovation, Technology and Circular Economy Fund (FITEC) (CIT/2018/15) and FCT for Andreia Silva fellowship (PD/BD/128363/2017) are gratefully acknowledged. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-14T22:16:18Z
2021-06
2021-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/119238
url http://hdl.handle.net/10362/119238
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-2607
PURE: 31821147
https://doi.org/10.3390/microorganisms9061149
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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